G. Schmuckler

Bio: G. Schmuckler is an academic researcher from Technion – Israel Institute of Technology. The author has contributed to research in topics: Polyacrylamide gel electrophoresis & Ion exchange. The author has an hindex of 5, co-authored 7 publications receiving 114 citations.

Structures and Reactivities of Platinum-Blues and the Related Amidate-Bridged Platinumiii Compounds

Abstract: Publisher Summary This chapter discusses structures and reactivities of platinum-blues and the related amidate-bridged platinumIII. Compounds platinumIII is usually unstable and can be isolated only in complexes with certain special ligands. Monomeric PtIII complex is very rare and so far only one well-characterized complex is known, in contrast to the now increasing knowledge of PtIII dinuclear complexes. The chapter mentions amidate-bridged dinuclear PtIII complexes and multinuclear PtII,III mixed-valent complexes, generally called “platinumblues.” The novel zigzag chain structures of the platinum blues having Pt-Pt bonds, redox chemistry between PtII and PtIII in these complexes electron spin resonance (ESR) spectra of Pt III, 195Pt-NMR of PtIII, and various stoichiometric and catalytic reactions with organic molecules mostly involving PtII/III redox reactions are summarized in the chapter. The antitumor inactive compounds are relatively stable and are disrupted only to dinuclear amidate-bridged compounds. The advantage of these complexes is that each Pt complex is isolated in pure form, and their crystal structure is known.

Comparison of batch and continuous multi-column protein A capture processes by optimal design.

Abstract: Multi-column capture processes show several advantages compared to batch capture. It is however not evident how many columns one should use exactly. To investigate this issue, twin-column CaptureSMB, 3- and 4-column periodic counter-current chromatography (PCC) and single column batch capture are numerically optimized and compared in terms of process performance for capturing a monoclonal antibody using protein A chromatography. Optimization is carried out with respect to productivity and capacity utilization (amount of product loaded per cycle compared to the maximum amount possible), while keeping yield and purity constant. For a wide range of process parameters, all three multi-column processes show similar maximum capacity utilization and performed significantly better than batch. When maximizing productivity, the CaptureSMB process shows optimal performance, except at high feed titers, where batch chromatography can reach higher productivity values than the multi-column processes due to the complete decoupling of the loading and elution steps, albeit at a large cost in terms of capacity utilization. In terms of trade-off, i.e. how much the capacity utilization decreases with increasing productivity, CaptureSMB is optimal for low and high feed titers, whereas the 3-column process is optimal in an intermediate region. Using these findings, the most suitable process can be chosen for different production scenarios.

Polymer supported iminodipropylene glycol functions for removal of boron

Abstract: Polymer supported iminodipropylene glycol functions have been shown to be efficient in chelation with boric acid and can be used for removal of boric acid at ppm levels. Glycidyl methacrylate (GMA)–methyl methacrylate (MMA)–DVB (divinyl benzene) terpolymer beads have been prepared and used as support. The polymer support with 3.4 mmol g−1 oxirane content can be readily modified by reacting with an excess of ethylene diamine, in high conversion yields (99.1%). Reaction of the latter with glycidol gives corresponding resin with aminopropylene glycol functions. The resulting resin has been demonstrated to be an efficient sorbent for removal of boron. The resin has 3 mmol g−1 of boron loading capacity and shows reasonably rapid sorption ability so that boron in 10 ml of H3BO3 solution (50 ppm) can be removed almost completely in less than 12 min of contact time by 0.5 g of polymer sample. Splitting of sorbed boron can be achieved by simple acid leaching (4 M HCl) and regenerated by NaOH solution (0.1 M).