Gabriela Ciavoi

Bio: Gabriela Ciavoi is an academic researcher from University of Oradea. The author has contributed to research in topics: Medicine & Dentistry. The author has an hindex of 3, co-authored 21 publications receiving 27 citations.

The Antioxidant Effect of Curcumin and Rutin on Oxidative Stress Biomarkers in Experimentally Induced Periodontitis in Hyperglycemic Wistar Rats.

Abstract: Background: There is a growing interest in the correlation between antioxidants and periodontal disease. In this study, we aimed to investigate the effect of oxidative stress and the impact of two antioxidants, curcumin and rutin, respectively, in the etiopathology of experimentally induced periodontitis in diabetic rats. Methods: Fifty Wistar albino rats were randomly divided into five groups and were induced with diabetes mellitus and periodontitis: (1) (CONTROL)—control group, (2) (DPP)—experimentally induced diabetes mellitus and periodontitis, (3) (DPC)—experimentally induced diabetes mellitus and periodontitis treated with curcumin (C), (4) (DPR)—experimentally induced diabetes mellitus and periodontitis treated with rutin (R) and (5) (DPCR)—experimentally induced diabetes mellitus and periodontitis treated with C and R. We evaluated malondialdehyde (MDA) as a biomarker of oxidative stress and reduced glutathione (GSH), oxidized glutathione (GSSG), GSH/GSSG and catalase (CAT) as biomarkers of the antioxidant capacity in blood harvested from the animals we tested. The MDA levels and CAT activities were also evaluated in the gingival tissue. Results: The control group effect was statistically significantly different from any other groups, regardless of whether or not the treatment was applied. There was also a significant difference between the untreated group and the three treatment groups for variables MDA, GSH, GSSG, GSH/GSSG and CAT. There was no significant difference in the mean effect for the MDA, GSH, GSSG, GSH/GSSG and CAT variables in the treated groups of rats with curcumin, rutin and the combination of curcumin and rutin. Conclusions: The oral administration of curcumin and rutin, single or combined, could reduce the oxidative stress and enhance the antioxidant status in hyperglycemic periodontitis rats.

Anti-Inflammatory and Antioxidant Properties of Carvacrol and Magnolol, in Periodontal Disease and Diabetes Mellitus.

Abstract: Periodontal disease and diabetes mellitus are two pathologies that are extremely widespread worldwide and share the feature of chronic inflammation. Carvacrol is a phenolic monoterpenoid, produced by a variety of herbs, the most well-known of which is Origanum vulgare. Magnolol is a traditional polyphenolic compound isolated from the stem bark of Magnolia officinalis, mainly used in Chinese medicine. The purpose of this paper is to review the therapeutic properties of these bioactive compounds, in the treatment of periodontitis and diabetes. Based on our search strategy we conducted a literature search in the PubMed and Google Scholar databases to identify studies. A total of one hundred eighty-four papers were included in the current review. The results show that carvacrol and magnolol have anti-inflammatory, antioxidant, antimicrobial, anti-osteoclastic, and anti-diabetic properties that benefit both pathologies. Knowledge of the multiple activities of carvacrol and magnolol can assist with the development of new treatment strategies, and the design of clinical animal and human trials will maximize the potential benefits of these extracts in subjects suffering from periodontitis or diabetes.

The Antimicrobial Activity of Origanum vulgare L. Correlated with the Gastrointestinal Perturbation in Patients with Metabolic Syndrome.

Abstract: (1) The metabolic syndrome (MS) promotes acute and chronic infections, due to the pro-inflammatory condition given by TNFα and IL6 or by affecting the microbiota. MS is also correlated with insulin resistance, causing inflammation and infections throughout the organism. (2) The purpose of this study was to track the effect of using the essential oil of Origanum vulgare L. (EOO) as an antibacterial treatment, compared to allopathic treatment with antibiotics in MS patients. A group of 106 people with MS was divided into four subgroups: L1-staphylococcal infection group, L2-Escherichia coli infection group, L3-streptococcal infection group with EOO treatment, and CG-control group without infection or treatment. (3) EOO is responsible for the antibacterial effect, and reduced minor uncomplicated infections. After a 10-day treatment, intestinal side effects were absent, improving the phase angle. (4) The results suggest that EOO may exhibit an antibacterial effect, similar to the antibiotic treatment, without promoting MS-specific dysbiosis, and it also improves the phase angle in patients, which is used as an index of health and cellular function.

Validity of the Demirjian Method for Dental Age Estimation in Romanian Children

Abstract: Dental age assessment is useful in various medical fields. The Demirjian method for dental age estimation is one of the most widely used in the field of pediatric dentistry. The aim of this study was to verify the accuracy of the Demirjian method in determining age in a sample of girls and boys from Oradea, Romania. This retrospective and radiographic study was based on the evaluation of 1006 panoramic radiographs, belonging to 1006 patients (431 boys and 575 girls) with ages between 3 and 13.9 years from the city of Oradea, Romania. They were collected from three private dental practices from Oradea and were analyzed between 1 September 2021, and 10 November 2021. The patients were distributed into 11 age groups, each group spanning over one year (e.g., 3–3.9, 4–4.9). For the assessment of dental age, the Demirjian method was used, which is based on the evaluation of the developmental stages of the lower left permanent teeth. The mean chronological age of the patients was 9.496 ± 2.218 years, and the mean dental age was 10.934 ± 2.585 years. The overestimation of dental age was obtained in all age groups for the entire sample. As such, dental age was higher than chronological age, with values varying from 0.34 years in the 3–3.9 years age group to 1.7 years in the 10–10.9 years age group. In girls, dental age was higher than chronological age, with values varying from 0.46 years in the 3–3.9 years age group to 1.73 years in the 11–11.9 years age group, while in boys, the values varied from 0.15 years in the first age group to 2.02 years in the 10–10.9 years age group. The comparison of the differences between chronological age and dental age according to the gender of the patients revealed that the distribution of the differences was nonparametric in both groups according to the Shapiro–Wilk test (p < 0.05). The differences between the groups were not significant according to the Mann–Whitney U test. However, larger differences were identified for boys (1.46 years) than girls (1.417 years), with an overestimation of the dental age. The Demirjian method overestimated the age of the children included in the investigated sample, with different values for the different age groups investigated, and requires adaptations.

Guided Bone Regeneration: biological principle and therapeutic applications

Abstract: The Guided Bone Regeneration (GBR) treatment concept advocates that regeneration of osseous defects is predictably attainable via the application of occlusive membranes, which mechanically exclude non-osteogenic cell populations from the surrounding soft tissues, thereby allowing osteogenic cell populations originating from the parent bone to inhabit the osseous wound. The present review discusses the evolution of the GBR biological rationale and therapeutic concept over the last two decades. Further, an overview of the GBR research history is provided with specific focus on the evidence available on its effectiveness and predictability in promoting the regeneration of critical size cranio-maxillo-facial defects, the neo-osteogenesis potential and the reconstruction of atrophic alveolar ridges before, or in conjunction with, the placement of dental implants. The authors conclude that future research should focus on (a) the investigation of the molecular mechanisms underlying the wound healing process following GBR application; (b) the identification of site and patient related factors which impact on the effectiveness and predictability of GBR therapy and (c) the evaluation of the pathophysiology of the GBR healing process in the presence of systemic conditions potentially affecting the skeletal system.To cite this article:Retzepi M, Donos N. Guided Bone Regeneration: biological principle and therapeutic applications.Clin. Oral Impl. Res. 21, 2010; 567-576.doi: 10.1111/j.1600-0501.2010.01922.x.

The British Journal of Dermatology: April 1913

Abstract: April 1913. Sutton, R. L. D i e K l a s s i f i k a t i o n d e r c h r o n i s c h e n r e s i s t e n t e n m a k u l S s e n und m a k u l o p a p u l S s e n s c h u p p e n d e n E r y t h r o d e r m i e n . p. 115. Der erste Fall S u t t o n s betrifft'eine 43j~hrige Frau, bei der die Hautaffektion vor 8 gahren in Form gelblichbrauner strichiSrmiger Verf~irbungen an Armen and Hiiften aufgetreten war. Die Herde wurden immer grS$er. Befallen sind lediglich die Extremitiiten. Die einzelnen Herde haben ovale Form, gelbliche oder rStliehe Farbe und schuppen ganz wenig. Die Herde liegen im Niveau der Haut. Schon klinisch 1KBt sich eine deutliehe Infiltration der Herde feststeUen. Mikroskopisch fanden sich folgende Ver~nderungen: Starke Verdiin.nung des Stratum granulosum, das Stratum lucidum fehlt. Odem der Stachelzellenschicht, welche auf 5--6 Zellsehichten reduziert ist. Schlechte F~irbbarkeit der Kerne und des Protoplasmas. Ausgesprochenes (3dem des Koriums. Die Papillen waren abgeflacht, aber weniger als bei einem typisehen Fall yon Erythrodermie pityriasique en plaques dissemin6es. Die. geschwellten Kollagenbiindel f~rben sieh schwaeh. Ausgesprochene Zellinfiltration besonders um die GefKl~e. Bei dem zweiten Fall, einem 23j~hrigen Manne, datiert die Krankheit seit 3 Jahren. Sie begann in der Leistengegend in Form kleiner roter Papeln, welche an Gr5~e zunahmen, bis Herde yon 5 cm Durchmesser entstanden. Sp~ter traten auch an anderen KSrperstellen solche tterde auf. Die u war grob symmetrisch; die affizierten Partien waren lichenoid, frei yon Schuppen oder wenig schuppend. Ausgesprochene Infiltration. Die Ver~nderungen an den Fingern~geln ~hnelten dem einen yon B r o c q s F~llen yon Parasporiasis. S u t t o n bezeiehnet den Fall als Psoriasis lichenoides. Er sehliel~t: Es gibt gewisse HautstSrungen, welche in grSBerem oder geringerem Grade die klinisehen Charakteristika der seborrhoischen Dermatitis, tier Psoriasis und des Lichen planus kombinieren. Der Kiirze wegen sollte man die Fi~lle unter das al]gemeine Kapitel der chronischen resistenten maku15sen und makulo-papulSsen schuppenden Erythrodermien zusammenfassen. Vom streng wissenschaftlichen Standpunkt ist es wahrseheinlich am besten, sie in versehiedene Gruppen zu tellen. Zu unterst gehSrt C r o c k e r s Xanthoerythroderm~a perstarts, zu oberst die Parakeratosis variegata yon Unna , S a n t i und P o l l i t z e r, mit B r o e q s Parapsoriasispruppe und die Psoriasis lichenoides, in welche letztere eingeschlossen ist N e i s s e r s lichenoide Eruption, J a d a s s o h n s psoriasiformes

Perspectives on the Combined Effects of Ocimum basilicum and Trifolium pratense Extracts in Terms of Phytochemical Profile and Pharmacological Effects.

Abstract: Nowadays, the tendency in pharmaceutical and food industries is to replace synthetic antioxidants with the natural ones. For this reason, there is a growing interest in analyzing natural, healthy and non-toxic additives as potential antioxidants. Some plants, which contain high levels of phenolic compounds, present an increasing interest for medicine due to their ability to scavenge free radicals, along with other pharmacological activities, such as antibacterial activity, wound healing and anti-inflammatory effect, to mention only a few. The aim of this review is to explore the therapeutic potential of Ocimum basilicum and Trifolium pratense in relation with their phytochemical profile and to highlight the pharmacological activity of aqueous or ethanol extracts. Special attention was devoted to the dermal pathology and wound healing effects, in the context of multiple skin conditions such as acne, eczema boils, psoriasis and rashes. Additionally, both extracts (Trifolium sp. and Ocimum sp.) are characterized by high content of antioxidant compounds, which are responsible for the radiance and resistance of the skin and slowing down of the aging process by maintaining estrogen levels. Moreover, the potential combined effect of the mixed extract is pointed out in terms of future applications for wound healing, based on some preliminary results obtained from a "scratch tests" assay performed with respect to human dermal fibroblasts.