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Gamal M. Mabrouk

Bio: Gamal M. Mabrouk is an academic researcher from Ain Shams University. The author has contributed to research in topics: Asphalt & Nigella sativa. The author has an hindex of 4, co-authored 4 publications receiving 162 citations.

Papers
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Journal Article
TL;DR: Supplementing diet with honey and Nigella sativa has a protective effect against MNU-induced oxidative stress, inflammatory response and carcinogenesis and abolished the NO and MDA elevations shown in sera of animals who did not receive these nutrients.
Abstract: We studied the protective effect of bee honey and Nigella grains as nutraceuticals on the oxidative stress and carcinogenesis induced by methylnitrosourea (MNU) in Sprague Dawely rats. Four groups of animals were used and fed ad-libitum. The first group was a control (n=8), the second (n=8), the third (n=15) and the fourth groups (n=12) were injected MNU (single i.v. dose 50 mg/kg body weight). After one week the third and fourth groups were given orally 0.2 g ground Nigella grains and 0.2 g Nigella with 5 g honey/rat/day, respectively. After six months all animals were sacrificed except two from the second group (MNU-injected rats) that died one-week before the end of the experiment. We observed that MNU injected in the second group produced a variety of oxidative stresses ranging from severe inflammatory reaction in lung and skin to colon adenocarcinoma in four out of six animals. There was an associated elevation of malondialdehyde (MDA) and nitric oxide (NO) in sera obtained from animals of this group compared to the control one. Nigella sativa grains given orally protected against MNU-induced oxidative stress and carcinogenesis by 80% (12/15) and combated this effect by lowering MDA and NO. Whereas honey from bees and Nigella sativa together protected 100% (12/12) against MNU-induced oxidative stress, carcinogenesis and abolished the NO and MDA elevations shown in sera of animals who did not receive these nutrients. These results showed that supplementation of diet with honey and Nigella sativa has a protective effect against MNU-induced oxidative stress, inflammatory response and carcinogenesis.

115 citations

Journal ArticleDOI
TL;DR: BH and NS are effective in reducing the viability of HepG2 cells, improving their antioxidant status and inducing their apoptotic death.
Abstract: Objectives: To evaluate in vitro antitumor effects of bee honey (BH) and Nigella sativa (NS) on HepG2 through their antioxidant and apoptotic activities. Methods: HepG2 cell line was treated with different concentrations of diluted unfractionated BH and different concentrations of alcohol extract of NS. Exposure lasted for different time durations (6-72 hours), both dose-response and time course-response were conducted. Cell viability was tested by trypan blue exclusion test. Total antioxidant status and caspase-3 activity were estimated in the cell lysate. Nitric oxide levels were measured in culture supernatants of both treated and untreated HepG2 at all indicated times. Results: Treatment of HepG2 cells with BH and NS leads to a significant decrease in both the number of viable HepG2 cells and the levels of nitric oxide on one hand, but improvement of the total antioxidant status and caspase-3 activity on the other, especially in HepG2 cells treated with higher doses of BH and NS (20% and 5000 μg/mL, respectively) and for longer duration (72 hours). Conclusions: BH and NS are effective in reducing the viability of HepG2 cells, improving their antioxidant status and inducing their apoptotic death.

50 citations

Journal ArticleDOI
TL;DR: In this paper , the effect of asphalt-binder high-temperature rheological properties on hot-mix asphalt rutting performance was investigated using the Texas flexible pavements and overlays database.
Abstract: Asphalt binder is one of the key constitutive components of hot-mix asphalt (HMA) that considerably affects its rutting performance. In particular, the high-temperature rheological properties measured from the multiple stress creep and recovery (MSCR) test are critical for correlating to the HMA rutting resistance. In this study, the Texas flexible pavements and overlays database was used as the data source to investigate the effect of asphalt-binder high-temperature rheological properties on the HMA rutting resistance. The study methodology was based on correlating the results of the MSCR test and the Hamburg wheel-tracking test (HWTT) to HMA field rutting performance. The data matrix for the study included asphalt binder (PG 64-22) from three different sources, three widely used Texas HMA mixes (fine gradation to coarse gradation), and five in-service highway test sections constructed using the same asphalt binders and HMA mixes. In general, the MSCR nonrecoverable creep compliance parameter, Jnrdiff, showed fairly strong correlations with the HMA rutting performance in the laboratory and field. The percent recovery parameter (R), on the other hand, exhibited the potential to ascertain and quantify the presence of modifiers in the asphalt binders. Furthermore, the test results indicated that material source/supplier has an impact on the rheological properties of the asphalt binders with the same performance grade (PG). Overall, the use of the MSCR test to quantify the asphalt-binder high-temperature rheological properties indicated the potential to compliment the laboratory HWTT test for correlating with the field HMA rutting performance in terms of the effects of asphalt binder.

8 citations

Journal ArticleDOI
TL;DR: The data suggest that TGF-β1 and cytochrome c may be useful prognostic markers that help patients' stratification and in adjusting the disciplines of therapy.

7 citations

Journal ArticleDOI
TL;DR: This paper presents a meta-analyses of the proton-probes of Na6(SO4, Na6, Na4, and Na4 of Na2(SO3) during the second week of June 2004 at the urging of the authors and obtained positive results for Na4 and Na2 during the first week of July.
Abstract: No Abstract. The Egyptian Journal of Biochemistry and Molecular Biology Vol. 22(2) 2004: 73-86

7 citations


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Journal ArticleDOI
TL;DR: The published findings provide clear evidence that both the oil and its active ingredients, in particular TQ, possess reproducible anti-oxidant effects through enhancing the oxidant scavenger system, which as a consequence lead to antitoxic effects induced by several insults.

767 citations

Journal ArticleDOI
TL;DR: Honey possesses antimicrobial capacity and anticancer activity against different types of tumors, acting on different molecular pathways that are involved on cellular proliferation.
Abstract: Honey is a natural substance appreciated for its therapeutic abilities since ancient times. Its content in flavonoids and phenolic acids plays a key role on human health, thanks to the high antioxidant and anti-inflammatory properties that they exert. Honey possesses antimicrobial capacity and anticancer activity against different types of tumors, acting on different molecular pathways that are involved on cellular proliferation. In addition, an antidiabetic activity has also been highlighted, with the reduction of glucose, fructosamine, and glycosylated hemoglobin serum concentration. Honey exerts also a protective effect in the cardiovascular system, where it mainly prevents the oxidation of low-density lipoproteins, in the nervous system, in the respiratory system against asthma and bacterial infections, and in the gastrointestinal system. A beneficial effect of honey can also be demonstrated in athletes. The purpose of this review is to summarize and update the current information regarding the role of honey in health and diseases.

366 citations

Journal Article
01 Jan 2004-Scopus
TL;DR: In this article, the effects of thymoquinone (TQ) against HCT-116 human colon cancer cells and attempted to identify its potential molecular mechanisms of action.
Abstract: For centuries, the black seed (Nigella sativa) herb and oil have been used in Asia, Middle East and Africa to promote health and fight disease. Thymoquinone (TQ), the most abundant constituent present in black seed, is a promising dietary chemopreventive agent. We investigated the effects of thymoquinone (TQ) against HCT-116 human colon cancer cells and attempted to identify its potential molecular mechanisms of action. We report that TQ inhibits the growth of colon cancer cells which was correlated with G1 phase arrest of the cell cycle. Furthermore, TUNEL staining and flow cytometry analysis indicate that TQ triggers apoptosis in a dose- and time-dependent manner. Apoptosis induction by TQ was associated with a 2.5-4.5-fold increase in mRNA expression of p53 and the downstream p53 target gene, p21WAF1. Simultaneously, we found a marked increase in p53 and p21WAF1 protein levels but a significant inhibition of anti-apoptotic Bcl-2 protein. Co-incubation with pifithrin-alpha (PFT-alpha), a specific inhibitor of p53, restored Bcl-2, p53 and p21WAF1 levels to the untreated control and suppressed TQ-induced cell cycle arrest and apoptosis. p53-null HCT-116 cells were less sensitive to TQ-induced growth arrest and apoptosis. These results indicate that TQ is antineoplastic and pro-apoptotic against colon cancer cell line HCT116. The apoptotic effects of TQ are modulated by Bcl-2 protein and are linked to and dependent on p53. Our data support the potential for using the agent TQ for the treatment of colon cancer.

301 citations

Journal ArticleDOI
TL;DR: In this paper, a review summarises the nutritional value, functional properties and nutraceutical applications of black cumin (Nigella sativa L.) oilseeds and its crude or essential oils have been widely used in traditional nutritional and medicinal applications.
Abstract: Summary Non-conventional seeds are being considered because their constituents have unique chemical properties and may augment the supply of nutritional and functional products. Black cumin (Nigella sativa L.) seeds and its crude or essential oils have been widely used in traditional nutritional and medicinal applications. Consequently, black cumin has been extensively studied for its nutritional value and biological activities. The black cumin oilseed had been shown to be anticancer, antidiabetic, antiradical and immunomodulator, analgesic, antimicrobial, anti-inflammatory, spasmolytic, bronchodilator, hepatoprotective, antihypertensive and renal protective. Moreover, black seeds have many antioxidative properties and activities. In consideration of potential utilisation, detailed knowledge on the composition of black cumin oilseed is of major importance. The diversity of applications to which black cumin can be put gives this oilseed great industrial importance. This review summarises the nutritional value, functional properties and nutraceutical applications of black cumin (N. sativa L.) oilseeds.

257 citations

Journal ArticleDOI
TL;DR: Findings that indicate honey may ameliorate oxidative stress in the gastrointestinal tract (GIT), liver, pancreas, kidney, reproductive organs and plasma/serum are presented and suggest that honey might be a novel antioxidant in the management of chronic diseases commonly associated with oxidative stress.
Abstract: The global prevalence of chronic diseases such as diabetes mellitus, hypertension, atherosclerosis, cancer and Alzheimer's disease is on the rise. These diseases, which constitute the major causes of death globally, are associated with oxidative stress. Oxidative stress is defined as an “imbalance between oxidants and antioxidants in favor of the oxidants, potentially leading to damage”. Individuals with chronic diseases are more susceptible to oxidative stress and damage because they have elevated levels of oxidants and/or reduced antioxidants. This, therefore, necessitates supplementation with antioxidants so as to delay, prevent or remove oxidative damage. Honey is a natural substance with many medicinal effects such as antibacterial, hepatoprotective, hypoglycemic, reproductive, antihypertensive and antioxidant effects. This review presents findings that indicate honey may ameliorate oxidative stress in the gastrointestinal tract (GIT), liver, pancreas, kidney, reproductive organs and plasma/serum. Besides, the review highlights data that demonstrate the synergistic antioxidant effect of honey and antidiabetic drugs in the pancreas, kidney and serum of diabetic rats. These data suggest that honey, administered alone or in combination with conventional therapy, might be a novel antioxidant in the management of chronic diseases commonly associated with oxidative stress. In view of the fact that the majority of these data emanate from animal studies, there is an urgent need to investigate this antioxidant effect of honey in human subjects with chronic or degenerative diseases.

251 citations