Author
Gary E. Gilbert
Other affiliations: Harvard University, Tufts University, Beth Israel Deaconess Medical Center ...read more
Bio: Gary E. Gilbert is an academic researcher from VA Boston Healthcare System. The author has contributed to research in topics: Lactadherin & Phosphatidylserine. The author has an hindex of 31, co-authored 79 publications receiving 4826 citations. Previous affiliations of Gary E. Gilbert include Harvard University & Tufts University.
Topics: Lactadherin, Phosphatidylserine, Binding site, Factor X, Prothrombinase
Papers published on a yearly basis
Papers
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TL;DR: A biosensor developed to study the subcellular distribution of phosphatidylserine found that it binds the cytosolic leaflets of the plasma membrane, as well as endosomes and lysosomes.
Abstract: Electrostatic interactions with negatively charged membranes contribute to the subcellular targeting of proteins with polybasic clusters or cationic domains. Although the anionic phospholipid phosphatidylserine is comparatively abundant, its contribution to the surface charge of individual cellular membranes is unknown, partly because of the lack of reagents to analyze its distribution in intact cells. We developed a biosensor to study the subcellular distribution of phosphatidylserine and found that it binds the cytosolic leaflets of the plasma membrane, as well as endosomes and lysosomes. The negative charge associated with the presence of phosphatidylserine directed proteins with moderately positive charge to the endocytic pathway. More strongly cationic proteins, normally associated with the plasma membrane, relocalized to endocytic compartments when the plasma membrane surface charge decreased on calcium influx.
945 citations
TL;DR: Results indicate that PADGEM mediates adhesion of activated platelets to monocytes and neutrophils and shares not only structural but also functional homology with ELAM-1 and MEL-14, members of a new family of vascular cell adhesion molecules.
888 citations
TL;DR: These studies indicate that activated platelets express a transient increase in high affinity receptors for factor VIII, whereas platelet-derived microparticles express a sustained increase in receptors.
278 citations
TL;DR: It is proposed that fVIII inhibition by anti-C2 antibodies is related to the overlap of their epitopes with the PS-binding site, and that some antibodies within the polyclonal anti-light chain population require only amino acids within C2 for binding.
192 citations
TL;DR: The membrane-binding properties that correlate to the anticoagulant capacity are characterized and lactadherin resembles factor VIII and V with stereoselective preference for phosphatidyl-L-serine and preference for highly curved membranes.
187 citations
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TL;DR: Three families of cell-surface molecules regulate the migration of lymphocytes and the interactions of activated cells during immune responses.
Abstract: The adhesive interactions of cells with other cells and with the extracellular matrix are crucial to all developmental processes, but have a central role in the functions of the immune system throughout life Three families of cell-surface molecules regulate the migration of lymphocytes and the interactions of activated cells during immune responses
6,595 citations
TL;DR: The key features of the life of a neutrophil are discussed, from its release from bone marrow to its death, and the mechanisms that are used by neutrophils to promote protective or pathological immune responses at different sites are explained.
Abstract: Neutrophils have traditionally been thought of as simple foot soldiers of the innate immune system with a restricted set of pro-inflammatory functions. More recently, it has become apparent that neutrophils are, in fact, complex cells capable of a vast array of specialized functions. Although neutrophils are undoubtedly major effectors of acute inflammation, several lines of evidence indicate that they also contribute to chronic inflammatory conditions and adaptive immune responses. Here, we discuss the key features of the life of a neutrophil, from its release from bone marrow to its death. We discuss the possible existence of different neutrophil subsets and their putative anti-inflammatory roles. We focus on how neutrophils are recruited to infected or injured tissues and describe differences in neutrophil recruitment between different tissues. Finally, we explain the mechanisms that are used by neutrophils to promote protective or pathological immune responses at different sites.
3,898 citations
TL;DR: Rolling of leukocytes on vascular endothelial cells, an early event in inflammation, can be reproduced in vitro on artificial lipid bilayers containing purified CD62, a selectin also named PADGEM and GMP-140 that is inducible on endothelial Cells.
2,242 citations
TL;DR: The composition of human milk is the biological norm for infant nutrition and contains many hundreds to thousands of distinct bioactive molecules that protect against infection and inflammation and contribute to immune maturation, organ development, and healthy microbial colonization.
1,859 citations
TL;DR: Recent findings show that the "traffic signals" for lymphocyte recirculation and for neutrophil and monocyte localization in inflammation are strikingly similar at the molecular level.
Abstract: The circulatory and migratory properties of white blood cells have evolved to allow efficient surveillance of tissues for infectious pathogens and rapid accu mulation at sites of injury and infection. Lymphocytes continually patrol the body for foreign antigen by recirculating from blood, through tissue, into lymph, and back to blood. Lymphocytes acquire a predilection, based on the environment in which they first encounter foreign antigen, to home to or recirculate through that same environment (39, 40). Granulocytes and mono cytes cannot recirculate, but emigrate from the bloodstream in response to molecular changes on the surface of blood vessels that signal injury or infec tion. Lymphocytes can similarly accumulate in response to inflammatory stim uli. The nature of the inflammatory stimulus determines whether lymphocytes. monocytes, neutrophils, or eosinophils predominate, and thus exercises spec ificity in the molecular signals or "area codes" that are displayed on endothe lium and control traffic of particular leukocyte classes. Recent findings show that the "traffic signals" for lymphocyte recirculation and for neutrophil and monocyte localization in inflammation are strikingly similar at the molecular level. These traffic signal or area code molecules are
1,570 citations