Gaurav Sharma

Bio: Gaurav Sharma is an academic researcher from Shenzhen University. The author has contributed to research in topics: Medicine & Photocatalysis. The author has an hindex of 82, co-authored 1244 publications receiving 31482 citations. Previous affiliations of Gaurav Sharma include Northeastern University & D. E. Shaw & Co..

Elimination of Hazard Cadmium Ions from Simulated Groundwater Using Hydroxyapatite Coated Filter Cake Made of Sewage Sludge and Cement Kiln Dust

Abstract: Novel sorbent named “coated filter cake by hydroxyapatite (CFCHAP)” that prepared from precipitation of hydroxyapatite nanoparticles on the filter cake was applied in permeable reactive barrier (PRB) technique. This barrier was tested by identification of its capability in the reclamation of simulated groundwater contaminated by cadmium ions. The filter cake is the material remaining from cement kiln dust and sewage sludge after calcium and phosphate extraction for manufacture of hydroxyapatite, respectively. Isotherm studies for CFCHAP–Cd interaction elucidated that Langmuir model is a suitable expression for representation of sorption measurements with determination coefficient (R2) > 0.99. The highest capacity of adsorption (qm) of CFCHAP is 34.722 mg/g which decreased slightly to 32.610 mg/g for mixture of 90% CFCHAP and 10% filter sand. The percentages of this mixture are suitable to fulfill the PRB requirements represented by acceptable hydraulic conductivity and reactivity in the continuous tests. As a function of the time, the experiments demonstrated a positive correlation between the longevity of the packed beds with the thickness of the PRB due to saturation with metal ions. The computerized solution by COMSOL 3.5a (2008) exhibited a good ability in describing the transportation of cadmium ions compared with experimental measurements. Measurements with model predictions signified that the cadmium propagation can be slowed at lower inlet concentration and water discharge for certain point along the bed.

Set Theoretic Quantization Index Modulation Watermarking

Abstract: We introduce a set theoretic framework for quantization index modulation (QIM) watermarking and illustrate its potency by designing a semi-fragile watermark that is both visually adaptive and tolerant to compression. We determine the watermarked image to satisfy the multiple constraints of watermark detectability, imperceptibility and robustness to compression using the method of projections onto convex sets (POCS). Mark embedding is performed through implicit quantization of statistical features, specifically the mean, of randomly selected pixel locations from the image. This is accomplished by defining a detectability constraint set that imposes the quantization constraint. We present experimental results demonstrating the efficacy of the technique in the presence of JPEG compression.

Draft Genome Sequence of Vibrio cyclitrophicus NCT10V, Cultivated from the Microbiome of a Marine Copepod.

Abstract: Here, we report the draft genome sequence of Vibrio cyclitrophicus NCT10V, cultivated from the copepod Acartia tonsa, collected from coastal surface waters of the western North Atlantic Ocean. The assembly is 5,638,575 bp long and has 44 contigs, a GC content of 43.2%, and 5,044 protein coding sequences.

Comparison of stabilisers for development of a lyophilised multiplex reverse-transcription PCR mixture for rapid detection of foot and mouth disease virus serotypes.

Abstract: Multiplex reverse-transcription polymerase chain reaction (mRT-PCR) assay is a sensitive and rapid method for the detection and serotyping of foot and mouth disease virus (FMDV) However, the method has not been used to its full potential, because of factors such as cost, a lack of infrastructure and the complexity of the reaction mixture This study was undertaken to optimise and validate a thermostable, lyophilised, ready-to-use mRT-PCR kit for the rapid detection of FMDV in field laboratories in India Trehalose, PEG-8000 and glycerol were evaluated for stabilisation of the PCR mixture at ambient temperatures The lyophilised mRT-PCR kit was validated and found robust enough for use in field-level laboratories The PCR reaction mixture in the ready-to-use kit has low complexity, so chances of cross-contamination during the preparation of the mixture are limited, but may easily be monitored by using lyophilised internal positive and negative controls In addition, the requirement to maintain live FMDV isolates as internal positive controls at field-level regional laboratories is eliminated

I and i

Abstract: There is, I think, something ethereal about i —the square root of minus one. I remember first hearing about it at school. It seemed an odd beast at that time—an intruder hovering on the edge of reality. Usually familiarity dulls this sense of the bizarre, but in the case of i it was the reverse: over the years the sense of its surreal nature intensified. It seemed that it was impossible to write mathematics that described the real world in …

SPAdes, a new genome assembly algorithm and its applications to single-cell sequencing ( 7th Annual SFAF Meeting, 2012)

Abstract: The lion's share of bacteria in various environments cannot be cloned in the laboratory and thus cannot be sequenced using existing technologies. A major goal of single-cell genomics is to complement gene-centric metagenomic data with whole-genome assemblies of uncultivated organisms. Assembly of single-cell data is challenging because of highly non-uniform read coverage as well as elevated levels of sequencing errors and chimeric reads. We describe SPAdes, a new assembler for both single-cell and standard (multicell) assembly, and demonstrate that it improves on the recently released E+V-SC assembler (specialized for single-cell data) and on popular assemblers Velvet and SoapDeNovo (for multicell data). SPAdes generates single-cell assemblies, providing information about genomes of uncultivatable bacteria that vastly exceeds what may be obtained via traditional metagenomics studies. SPAdes is available online ( http://bioinf.spbau.ru/spades ). It is distributed as open source software.