Gayatri Acharya

Bio: Gayatri Acharya is an academic researcher from Utkal University. The author has contributed to research in topics: Clarias & Heteropneustes fossilis. The author has an hindex of 3, co-authored 4 publications receiving 27 citations.

Comparative haematological and serum biochemical analysis of catfishes Clarias batrachus (Linnaeus, 1758) and Heteropneustes fossilis (Bloch, 1794) with respect to sex

Abstract: Aim of this study was to investigate the haematological and serum biochemical parameters of two catfishes Heteropneustes fossilis (Bloch, 1794) and Clarias batrachus (Linnaeus, 1758) of Odisha. In this study, the estimated values were compared between the two species of catfishes as well as with respect to sex. Haematological parameters such as haemoglobin (Hb), total erythrocyte count (TEC), total leucocyte count(TLC), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), and mean corpuscular haemoglobin Concentration (MCHC) and the serum biochemical parameters like protein, albumin, globulin, glucose and cholesterol were measured and compared between and within the species as well as with respect to sex using One-Way Analysis of Variance (ANOVA) followed by Turkey’s pair wise comparison tests. All haematological parameters differ significantly (p<0.001) between C. batrachus and H. fossilis except PCV and MCV. Except albumin all biochemical parameters differ significantly between C. batrachus and H. fossilis.

Comparative haematology of Anas platyrhynchos (Anseriformes) and Coturnix coturnix Japonica (Galliformes)

Abstract: Haematological study of adult female Indian runner duck and Japanese quail was conducted to evaluate the variation between two different poultry birds having different habitat. Results obtained in case of Japanese quail showed a higher total leucocyte count (p<0.05), lower haemoglobin value (p<0.001) and erythrocyte count (p<0.05) in comparison to Indian runner duck. Aggregates of RBC and deformed monocytes were also observed. This present study will be helpful in better understanding of different poultry birds having different habitats.

Effect of sex on haemocytobiochemical profiling of silver tiger fish (Datnioides polota Hamilton, 1822)

Abstract: This investigation presents an overview of the effect of sex on haematobiochemical and haemocytomorphometrical aspects of Datnioides polota. For the present study, 15 males and 15 females of Datnioides polota were collected from Balugaon, the central sector of Chilika lagoon of Odisha. Two millilitres of blood samples was collected from each sex through the caudal vein. One millilitre of collected blood was transferred from the syringe into ethylenediaminetetraacetic acid (EDTA) containing vials, for haematological studies and other 1 ml was taken in Eppendorf tube without anticoagulant for analyses of biochemical parameters. Haematological parameters like red blood cell count (RBC), white blood cells count (WBC), haemoglobin, haematocrit, mean cell volume (MCV), mean cell haemoglobin (MCH), mean cell haemoglobin concentration (MCHC), biochemical parameters such as glucose, protein, albumin, globulin, cholesterol, and morphometrical parameters namely length, breadth, area, and N/C ratio of erythrocytes, lymphocytes, monocytes, eosinophils, and neutrophils are analysed in relation to the sex of the fish. Statistical analysis reveals the sex wise significant difference in some haematological parameters like Hb which varies at P < 0.01 level while WBC deviate at P < 0.05 level. All the biochemical parameters show higher value in females in comparison to males except albumin but sex wise significant variation recorded in protein and globulin at P < 0.01 level. In males, the concentration of Hb is positively correlated with RBC and PCV while in females negatively correlated. RBC is positively correlated with PCV in both the sexes at R2 = 0.008 and R2 = 0.9 in male and female, respectively. The morphometry of blood cells such as monocytes and lymphocytes shows significant sexual dimorphism. The cellular length and area of monocytes deviate at the level of P < 0.01 and breadth at P < 0.05 level. The cellular and nuclear breadth of lymphocyte vary significantly at the level of P < 0.05 and P < 0.001, respectively. The results of this study may serve as a reference value for haemocytological and biochemical parameters of this species which in turn helps in diagnosing diseases and increasing fish production.

Comparative study of haematology of two teleost fish (Mugil cephalus and Carassius auratus) from different environments and feeding habits

Abstract: Haematological parameters are valuable indicators of fish health status. This study is aimed to provide baseline data of the blood profile of two teleost fish species living in different environmen...

Avian and Exotic Animal Hematology and Cytology

Abstract: This book is a comprehensive guide and manual that is an essential component for the library of any in-house or reference laboratory that evaluates specimens of avian and exotic animal species. In addition, this book is a valuable asset to clinicians in the field because it provides detailed illustrations and descriptions on collection and preparation of tissue and bodyfluid samples from birds, reptiles, and small mammals. The writing is clear and consistently organized throughout, with a comprehensive index that provides easy and rapid access to pertinent information. The book is composed of seven chapters. The first five chapters (138 pages) are dedicated to the hematology of birds, reptiles, amphibians, fish, and exotic / laboratory small mammals. The last two chapters (pages 139 to 238) describe the collection, preparation, and cytological evaluation of various tissue specimens and body fluids from avian and exotic animal species. The book also contains two appendices, the first of which describes the preparation of materials and the staining procedures for several common stains and solutions used in hematology and cytology. This appendix also provides a brief explanation of the interpretation of results used with each stain. The second appendix consists of a series of tables providing hematologic reference intervals for selected avian, reptile, amphibian, and small mammal exotic species. Each table is a compilation of data from previously published reference intervals. These are extremely valuable tools, particularly for practicing clinicians. Most exotic animal clinicians work with a number of species, and finding reliable reference intervals is challenging, which makes having these in a single source of reference invaluable. The first five chapters on hematology are organized in a similar manner. Each begins with a brief introduction to the hematology of the species (avian, reptile, etc.) followed by sections describing and illustrating the various methods of restraint, blood collection, and sample preparation. The authors’ experience in both collection and evaluation of samples is evident in the details that they provide in each chapter. The authors not only describe the various methods of sample collection, but also provide information regarding indications for using each of the various methods. Photographs of excellent quality illustrate proper restraint and sample collection. Descriptions of the procedures are fully detailed in all hematology chapters, but are particularly noteworthy in the chapters covering avian and reptile species. The sections on sample collection in these first five chapters are followed by a discussion of various anticoagulants. The authors point out species differences with regard to proper anticoagulant, spelling out why some anticoagulants are not suitable for certain exotic animal species. The chapter on avian hematology (Chapter 1) also details, by text and images, the proper methods for blood-film preparation. The sections on restraint and sample collection are complemented by material describing laboratory methods of sample evaluation such as quantitative analysis of blood cells and qualitative analysis of blood cell morphology. This topic is particularly well detailed in the avian chapter, but is adequately covered in other chapters as well. This information is appropriate, since many of the principles described in the avian section may be applied across animal classes, particularly nonmammalian species with nucleated erythrocytes. Each chapter (1–5) concludes with a detailed description and photomicrograph illustrations of the normal and abnormal erythrocyte, leukocyte, and thrombocyte morphology, followed by a section describing various hemoparasites seen in each species. The text in these sections is extensive, particularly for the avian and reptile species, and the authors not only describe normal and abnormal findings but also provide valuable information on the clinical significance of various abnormalities, as they relate to specific diseases in each species. The photomicrograph illustrations of the various blood cells and blood cell abnormalities are comprehensive. The quality of these illustrations is good and sometimes excellent, but always of adequate clarity (focus) and size to allow easy evaluation of normal and abnormal cell morphology and hemoparasite identification. Some of the images have a yellow tint that may slightly alter the true color, but this does not interfere with the overall accuracy and usefulness of the image. The chapters describing amphibian, fish, and exotic small mammal hematology are not as extensive as the chapters on avian and reptile hematology, which is understandable

Comparative study of hematological parameters of snow trout Schizopyge plagiostomus and Schizopyge niger inhabiting two different habitats

Abstract: The aim of the present study is to compare the hematological parameters of two snow trout fish species, Schizopyge plagiostomus and Schizopyge niger, inhabiting two different habitats, to e...

SDS-PAGE technique as biomarker for fish toxicological studies.

Abstract: Although many studies on the hematological and biochemical parameters in fishes have been done, still there are some shortage in the estimation and evaluation of the baseline’s values of marine and freshwater fishes. Recently, the use of hematology and biochemistry of fishes in toxicology, aquaculture, environmental pollution, feeding, and antioxidants studies has been increased. In this study we introduced the importance of those parameters and their importance as biomarkers in fish toxicology from previous literature and as new findings. Hemato-biochemical parameters were widely used in fish toxicological studies. Many researches have used the protein electrophoresis as a valid tool to determining intra and inter-specific variation among species. Protein profile was extensively used in determining the health of fish, as indicators of anemia or other diseases provide information about the existence of the disease, and in the diagnosis of disease. So, to carry out the aim of this study, we reported one of the more advanced techniques used SDS-PAGE as molecular biomarker for protein profile analysis in fish with shedding the light on the importance of hematological and biochemical parameters in fish toxicological studies.