Gene-Wei Li

TL;DR: System-wide analyses of protein and mRNA expression in individual cells with single-molecule sensitivity using a newly constructed yellow fluorescent protein fusion library for Escherichia coli found that almost all protein number distributions can be described by the gamma distribution with two fitting parameters which, at low expression levels, have clear physical interpretations as the transcription rate and protein burst size.

TL;DR: Using an EGFP-tagged endonuclease-deficient Cas9 protein and a structurally optimized small guide (sg) RNA, robust imaging of repetitive elements in telomeres and coding genes in living cells is demonstrated by repurposing the bacterial CRISPR/Cas system.

TL;DR: This work presents a genome-wide approach, based on ribosome profiling, for measuring absolute protein synthesis rates, and reveals how general principles, important both for understanding natural systems and for synthesizing new ones, emerge from quantitative analyses of protein synthesis.

TL;DR: In a living Escherichia coli cell, specific binding of a lac repressor, labeled with a fluorescent protein, to a chromosomal lac operator is observed and the kinetics of binding and dissociation of the repressor in response to metabolic signals are measured using single-molecule detection techniques.

TL;DR: It is shown that internal SD-like sequences are a major determinant of translation rates and a global driving force for the coding of bacterial genomes, and that codons decoded by rare transfer RNAs do not lead to slow translation under nutrient-rich conditions.