G
Gerald Schwank
Researcher at University of Zurich
Publications - 50
Citations - 5529
Gerald Schwank is an academic researcher from University of Zurich. The author has contributed to research in topics: Genome editing & Biology. The author has an hindex of 18, co-authored 37 publications receiving 4100 citations. Previous affiliations of Gerald Schwank include Utrecht University & Royal Netherlands Academy of Arts and Sciences.
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Journal ArticleDOI
Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients
Gerald Schwank,Bon-Kyoung Koo,Valentina Sasselli,Johanna F. Dekkers,Inha Heo,Turan Demircan,Nobuo Sasaki,Sander Boymans,Edwin Cuppen,Cornelis K. van der Ent,Edward E. S. Nieuwenhuis,Jeffrey M. Beekman,Hans Clevers +12 more
TL;DR: The CRISPR/Cas9 genome editing system is used to correct the CFTR locus by homologous recombination in cultured intestinal stem cells of CF patients and the corrected allele is expressed and fully functional as measured in clonally expanded organoids.
Journal ArticleDOI
Sequential cancer mutations in cultured human intestinal stem cells
Jarno Drost,Richard H. van Jaarsveld,Bas Ponsioen,Cheryl Zimberlin,Ruben van Boxtel,Arjan Buijs,Norman Sachs,René M. Overmeer,G. Johan A. Offerhaus,Harry Begthel,Jeroen Korving,Marc van de Wetering,Gerald Schwank,Meike Logtenberg,Edwin Cuppen,Hugo J. Snippert,Jan Paul Medema,Geert J. P. L. Kops,Hans Clevers +18 more
TL;DR: CRISPR/Cas9 technology is utilized for targeted gene modification of four of the most commonly mutated colorectal cancer genes (APC, P53, KRAS and SMAD4) in cultured human intestinal stem cells to achieve Mutant organoids that remain genetically and phenotypically stable.
Journal ArticleDOI
Tissue-specific mutation accumulation in human adult stem cells during life
Francis Blokzijl,Joep de Ligt,Myrthe Jager,Valentina Sasselli,Sophie Roerink,Nobuo Sasaki,Meritxell Huch,Sander Boymans,Ewart W. Kuijk,Pjotr Prins,Isaac J. Nijman,Inigo Martincorena,Michal Mokry,Caroline L. Wiegerinck,Sabine Middendorp,Toshiro Sato,Gerald Schwank,Edward E. S. Nieuwenhuis,Monique M A Verstegen,Luc J. W. van der Laan,Jeroen de Jonge,Jan N. M. IJzermans,Robert G.J. Vries,Marc van de Wetering,Michael R. Stratton,Hans Clevers,Edwin Cuppen,Ruben van Boxtel +27 more
TL;DR: This work determines genome-wide mutation patterns in ASCs of the small intestine, colon and liver of human donors with ages ranging from 3 to 87 years by sequencing clonal organoid cultures derived from primary multipotent cells.
Journal ArticleDOI
Auxin triggers transient local signaling for cell specification in Arabidopsis embryogenesis.
Dolf Weijers,Alexandra Schlereth,Jasmin S. Ehrismann,Gerald Schwank,Marika Kientz,Gerd Jürgens +5 more
TL;DR: The results suggest that hypophysis specification is not the direct result of a primary auxin response but rather depends on cell-to-cell signaling triggered by auxin in adjacent cells.
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Treatment of a metabolic liver disease by in vivo genome base editing in adult mice
Lukas Villiger,Hiu Man Grisch-Chan,Helen Lindsay,Helen Lindsay,Femke Ringnalda,Chiara Balbo Pogliano,Gabriella Allegri,Ralph Fingerhut,Johannes Häberle,Johannes Häberle,Joao Matos,Mark D. Robinson,Mark D. Robinson,Beat Thöny,Beat Thöny,Gerald Schwank +15 more
TL;DR: AAV-mediated base editing corrects an autosomal recessive mutation in the Pahenu2 gene and ameliorates molecular deficits in a mouse model of metabolic liver disease and suggests that targeting genetic diseases in vivo using AAV-mediated delivery of base-editing agents is feasible.