Showing papers by "Giuseppe Coppola published in 2021"
TL;DR: In this article, the effect of a one-step sulfur hexafluoride (SF6) plasma treatment upon the low velocity impact behavior of basalt/epoxy composites has been investigated by using several characterization techniques.
Abstract: Hydrophobic surfaces are highly desired for several applications due to their exceptional properties such as self-cleaning, anti-icing, anti-friction and others. Such surfaces can be prepared via numerous methods including plasma technology, a dry technique with low environmental impact. In this paper, the effect of a one-step sulfur hexafluoride (SF6) plasma treatment upon the low velocity impact behavior of basalt/epoxy composites has been investigated by using several characterization techniques. A capacitive coupled radiofrequency plasma system was used for the plasma surface treatment of basalt/epoxy composites, and suitable surface treatment conditions were experimentally investigated with respect to gas flow rate, chamber pressure, power intensity, and surface treatment time by measuring the water droplet contact angle of treated specimens. The contact angle measurements showed that treating with SF6 plasma would increase the hydrophobicity of basalt/epoxy composites; moreover, the impact results obtained on reinforced epoxy basalt fiber showed damage in a confined area and higher impact resistance for plasma-treated basalt systems.
19 citations
TL;DR: In this paper, the authors proposed a method called Luo-N-Fabs, which consists of an antigen binding fragment of a full-length antibody (Fab/ fragment antigen binding) that is selectively conjugated at the N-terminal amino group with fluorescent organic molecules.
Abstract: Currently, a major technical limitation of microscopy based image analysis is linkage error – a visualization error that is measured by the distance between the cellular protein to be detected and the fluorescence emitter used for detection. With continuously improving resolution of today′s (super-resolution) microscopes, the linkage errors can severely hamper the correct interpretation of images and is usually introduced in experiments by the use of standard intracellular staining reagents such as fluorescently labelled antibodies. The linkage error of standard labelled antibodies is caused by the size of the antibody and the random distribution of fluorescent emitters on the antibody surface. Together, these two factors account for a fluorescence displacement of ~40nm when staining proteins by indirect immunofluorescence; and ~20nm when staining with fluorescently coupled primary antibodies. In this study, we describe a class of staining reagents that effectively reduce the linkage error by more than five-fold when compared to conventional staining techniques. These reagents, called Fluo–N–Fabs, consist of an antigen binding fragment of a full-length antibody (Fab/ fragment antigen binding) that is selectively conjugated at the N-terminal amino group with fluorescent organic molecules, thereby reducing the distance between the fluorescent emitter and the protein target of the analysis. Fluo–N–Fabs also exhibit the capability to penetrate tissues and highly crowded cell compartments, thus allowing for the efficient detection of cellular epitopes of interest in a wide range of fixed samples. We believe this class of reagents realize an unmet need in cell biological super resolution imaging studies where the precise localization of the target of interest is crucial for the understanding of complex biological phenomena.