Guan Zhi

Bio: Guan Zhi is an academic researcher. The author has contributed to research in topics: Michael reaction & Lipase. The author has an hindex of 1, co-authored 1 publications receiving 2 citations.

Method for catalytic synthesis of dicoumarin compound through lipases

Abstract: The invention discloses a method for catalytic synthesis of a dicoumarin compound through lipases. The method comprises the step: 4-hydroxycoumarin is adopted as a substrate, and subjected to a Knoevenagel-Michael cascade reaction through lipase catalyzing to generate the dicoumarin compound with a compound in the formula II. The dicoumarin compound can be used for treating thrombus, resisting HIV, and treating asthma, skin cancer and snake venom, and can further be used for antimicrobial treatment of certain staphylococcus aureus and staphylococcus epidermidis and preparing of analytical reagents; and according to the method, reacting conditions are mild, aftertreatment is easy, the substrate is widespread, a catalyst can be recycled, and the method is an economical and environment-friendly new method for synthesizing the dicoumarin compound.

Method for preparing catalyst for biodiesel production

Abstract: The invention discloses a method for preparing a catalyst for biodiesel production. The method comprises the steps of 1, cutting naturally air-dried or baked snake gourds which grow and mature normally into cylindrical bodies which are 1-5 cm long, conducting peeling, and removing seeds inside pores, so that cylindrical loofah sponges are obtained; 2, placing the cylindrical loofah sponges in a lipase solution to be soaked for 8-24 h at 20-50 DEG C, and adding an activator solution at the same time, wherein the activator is a mixture of N,N-dicyclohexylcarbodiimide, N-hydroxysuccinimide and dimethyl acetylenedicarboxylate based on the substance amount ratio of 1:(0.5-2):(0.05-0.2); and 3, drying the loofah sponges loaded with lipase to obtain the catalyst. According to the preparing method, the loofah sponges are used as a carrier, and lipase immobilization is achieved through physical absorption and covalent coupling.