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Guangsheng Du

Bio: Guangsheng Du is an academic researcher from Sichuan University. The author has contributed to research in topics: Immune system & Medicine. The author has an hindex of 12, co-authored 24 publications receiving 428 citations. Previous affiliations of Guangsheng Du include Leiden University & Tianjin University.

Papers
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Journal ArticleDOI
TL;DR: The promise of lymph node-targeting large-pore MSNs as vaccine-delivery vehicles for immune activation and cancer vaccination is demonstrated.
Abstract: Subunit vaccines generally proceed through a 4-step in vivo cascade—the DUMP cascade—to generate potent cell-mediated immune responses: (1) drainage to lymph nodes; (2) uptake by dendritic cells (DCs); (3) maturation of DCs; and (4) Presentation of peptide-MHC I complexes to CD8+ T cells How the physical properties of vaccine carriers such as mesoporous silica nanoparticles (MSNs) influence this cascade is unclear We fabricated 80-nm MSNs with different pore sizes (78 nm, 103 nm, and 129 nm) and loaded them with ovalbumin antigen Results demonstrated these MSNs with different pore sizes were equally effective in the first three steps of the DUMP cascade, but those with larger pores showed higher cross-presentation efficiency (step 4) Consistently, large-pore MSNs loaded with B16F10 tumor antigens yielded the strongest antitumor effects These results demonstrate the promise of our lymph node-targeting large-pore MSNs as vaccine-delivery vehicles for immune activation and cancer vaccination

121 citations

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TL;DR: It is demonstrated that ZANPs may be a safe and effective vehicle for the development of cancer vaccines by taking advantage of a natural biomineralization process to create aluminum-containing nanoZIF-8 particles for antigen delivery.

110 citations

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TL;DR: The applicator controlled hollow microneedle delivery is an excellent method for intradermal injection of nanoparticle vaccines, allowing selection of optimal nanoparticle formulations for humoral and cellular immune responses.

103 citations

Journal ArticleDOI
TL;DR: Several critical formulation parameters are identified for the further development of NP‐loaded dissolving MNs loaded with PLGA nanoparticles co‐encapsulating ovalbumin (OVA) and poly(I:C) for intradermal immunisation.

59 citations

Journal ArticleDOI
TL;DR: Al-BSA-Ce6 NPs is presented as a novel and unique system for alumina adjuvants that serves as an effective approach for cancer therapy.

57 citations


Cited by
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Journal ArticleDOI
TL;DR: The relationship between the structure and properties of MOF is revealed and a better understanding of these release mechanisms under different stimuli would benefit the designing of sophisticated DDSs based on the promising material.

303 citations

Journal ArticleDOI
TL;DR: This work presents a new microfabrication technique for MNs using fused deposition modeling (FDM) 3D printing using polylactic acid, an FDA approved, renewable, biodegradable, thermoplastic material, and demonstrates how the swellability of PLA can be exploited to load small molecule drugs and how its degradability in skin can release those small molecules over time.
Abstract: Biodegradable polymer microneedle (MN) arrays are an emerging class of transdermal drug delivery devices that promise a painless and sanitary alternative to syringes; however, prototyping bespoke needle architectures is expensive and requires production of new master templates. Here, we present a new microfabrication technique for MNs using fused deposition modeling (FDM) 3D printing using polylactic acid, an FDA approved, renewable, biodegradable, thermoplastic material. We show how this natural degradability can be exploited to overcome a key challenge of FDM 3D printing, in particular the low resolution of these printers. We improved the feature size of the printed parts significantly by developing a post fabrication chemical etching protocol, which allowed us to access tip sizes as small as 1 μm. With 3D modeling software, various MN shapes were designed and printed rapidly with custom needle density, length, and shape. Scanning electron microscopy confirmed that our method resulted in needle tip sizes in the range of 1-55 μm, which could successfully penetrate and break off into porcine skin. We have also shown that these MNs have comparable mechanical strengths to currently fabricated MNs and we further demonstrated how the swellability of PLA can be exploited to load small molecule drugs and how its degradability in skin can release those small molecules over time.

207 citations

Journal ArticleDOI
TL;DR: In this review, a great effort has been made to summarize the advance of microneedles including their materials and latest fabrication method, such as three-dimensional printing (3DP).

203 citations

Journal ArticleDOI
TL;DR: Insulin MNs are promising for painless diabetes therapeutics, and additional efforts for addressing fundamental issues including the drug loading, the PK/PD profile, the storage and the safety of insulin MNs will accelerate the clinical transformation.

186 citations

Journal ArticleDOI
TL;DR: The synergistic mechanism of HTT in immunotherapy, including immunogenic cell death and reversal of the immunosuppressive tumor microenvironment is discussed, and these strategies could achieve synergistically enhanced therapeutic outcomes against both primary tumors and metastatic lesions, prevent cancer recurrence, and prolong the survival period.
Abstract: The past decades have witnessed hyperthermia therapy (HTT) as an emerging strategy against malignant tumors. Nanomaterial-based photothermal therapy (PTT) and magnetic hyperthermia (MHT), as highly effective and noninvasive treatment models, offer advantages over other strategies in the treatment of different types of tumors. However, both PTT and MHT cannot completely cure cancer due to recurrence and distal metastasis. In recent years, cancer immunotherapy has attracted widespread attention owing to its capability to activate the body's own natural defense to identify, attack, and eradicate cancer cells. Significant efforts have been devoted to studying the activated immune responses caused by hyperthermia-ablated tumors. In this article, the synergistic mechanism of HTT in immunotherapy, including immunogenic cell death and reversal of the immunosuppressive tumor microenvironment is discussed. The reports of the combination of HTT or HTT-based multimodal therapy with immunotherapy, including immunoadjuvant exploitation, immune checkpoint blockade therapy, and adoptive cellular immunotherapy are summarized. As highlighted, these strategies could achieve synergistically enhanced therapeutic outcomes against both primary tumors and metastatic lesions, prevent cancer recurrence, and prolong the survival period. Finally, current challenges and prospective developments in HTT-synergized immunotherapy are also reviewed.

171 citations