Gustavo Caetano-Anollés

Bio: Gustavo Caetano-Anollés is an academic researcher from University of Illinois at Urbana–Champaign. The author has contributed to research in topics: Protein domain & Phylogenetic tree. The author has an hindex of 51, co-authored 240 publications receiving 12873 citations. Previous affiliations of Gustavo Caetano-Anollés include Ohio State University & University of Tennessee.

DNA amplification fingerprinting using very short arbitrary oligonucleotide primers.

Abstract: The surprising finding that amplification of genomic DNA can be directed by only one oligonucleotide primer of arbitrary sequence to produce a characteristic spectrum of short DNA products of varying complexity, was applied as a strategy to detect genetic differences between organisms. This approach, DNA amplification fingerprinting (DAF), does not depend on cloning or DNA sequence information and can generate fingerprints from DNA of viral, bacterial, fungal, plant and animal origins. Primers as short as 5 nucleotides in length can produce complex banding patterns that are resolved by polyacrylamide gel electrophoresis and silver staining. Amplification fragment length polymorphisms (AFLPs) were detected between different human individuals as well as between soybean cultivars. It is anticipated that DAF will have wide application for DNA analysis.

Extensive and specific responses of a eukaryote to bacterial quorum-sensing signals

Abstract: Many bacteria use N-acyl homoserine lactone (AHL) signals to coordinate the behavior of individual cells in a local population. The successful infection of eukaryotic hosts by bacteria seems to depend particularly on such AHL-mediated “quorum-sensing” regulation. We have used proteome analysis to show that a eukaryotic host, the model legume Medicago truncatula, is able to detect nanomolar to micromolar concentrations of bacterial AHLs from both symbiotic (Sinorhizobium meliloti) and pathogenic (Pseudomonas aeruginosa) bacteria, and that it responds in a global manner by significant changes in the accumulation of over 150 proteins, 99 of which have been identified by peptide mass fingerprinting. The accumulation of specific proteins and isoforms depended on AHL structure, concentration, and time of exposure. AHLs were also found to induce tissue-specific activation of β-glucuronidase (GUS) reporter fusions to an auxin-responsive and three chalcone synthase promoters, consistent with AHL-induced changes in the accumulation of auxin-responsive and flavonoid synthesis proteins. In addition, exposure to AHLs was found to induce changes in the secretion of compounds by the plants that mimic quorum-sensing signals and thus have the potential to disrupt quorum sensing in associated bacteria. Our results indicate that eukaryotes have an extensive range of functional responses to AHLs that may play important roles in the beneficial or pathogenic outcomes of eukaryote–prokaryote interactions.

Plant Genetic Control of Nodulation

Abstract: INTRODUCTION 345 LEGUME NODULATION . . ........ ....... ... .. ... . . ....... ..... ........ . 346 ROLE OF THE BACTERIAL SYMBIONT: SIGNAL EXCHANGE 348 NODULATION AS A DEVELOPMENTALLY REGULATED PHENOMENON....... 354 PLANT NODULATION MUTANTS 357 NODULATION CONTROL IN LEGUMES 361 AUTOREGULATION ...... ........ 362 What Is the Signal Transduction Mechanism? an Example of Systemic Root-Shoot Interaction .... .. . .. .... .. ....... ""'''''''''''''''''''''''''' .. " .. ,," 363 How Is Nodulation Suppressed? " 364 What Triggers the Systemic Response?. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 365 A Working Hypothesis. . . . . . . . . . . . . . . . . . . , . . . . . . . . . . . . . . . . . . . ..... . . . . . . . . . . . . . ... .. . 367 NODULATION IN THE ABSENCE OF RHIZOBIUM SPP. 368 PERSPECTIVES 370

DNA markers : protocols, applications, and overviews

Abstract: Partial table of contents: PROTOCOLS. Molecular Hybridization (D. Grant & R. Shoemaker). Hybridization--Based Microsatellite Fingerprinting of Plants and Fungi (K. Weising & G. Kahl). Randomly Amplified Polymorphic DNA (RAPD) Analysis (J. Rafalski). AFLP Analysis (P. Vos & M. Kuiper). Simple Sequence Repeat DNA Marker Analysis (P. Cregan & C. Quigley). Genomic Fingerprinting Using Oligonucleotide Arrays (K. Beattie). APPLICATIONS. Molecular Markers and Forest Trees (D. O'Malley & R. Whetten). Cultivar Identification and Varietal Protection (S. Smith). Arbitrarily Amplified DNA in Ecology and Evolution (B. Schierwater, et al.). Appendix. Glossary. Index.

疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A

Abstract: 抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1（PfPMP1）与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员，通过启动转录不同的var基因变异体为抗原变异提供了分子基础。

AFLP: a new technique for DNA fingerprinting.

Abstract: A novel DNA fingerprinting technique called AFLP is described. The AFLP technique is based on the selective PCR amplification of restriction fragments from a total digest of genomic DNA. The technique involves three steps: (i) restriction of the DNA and ligation of oligonucleotide adapters, (ii) selective amplification of sets of restriction fragments, and (iii) gel analysis of the amplified fragments. PCR amplification of restriction fragments is achieved by using the adapter and restriction site sequence as target sites for primer annealing. The selective amplification is achieved by the use of primers that extend into the restriction fragments, amplifying only those fragments in which the primer extensions match the nucleotides flanking the restriction sites. Using this method, sets of restriction fragments may be visualized by PCR without knowledge of nucleotide sequence. The method allows the specific co-amplification of high numbers of restriction fragments. The number of fragments that can be analyzed simultaneously, however, is dependent on the resolution of the detection system. Typically 50-100 restriction fragments are amplified and detected on denaturing polyacrylamide gels. The AFLP technique provides a novel and very powerful DNA fingerprinting technique for DNAs of any origin or complexity.

Random graphs

Abstract: We will review some of the major results in random graphs and some of the more challenging open problems. We will cover algorithmic and structural questions. We will touch on newer models, including those related to the WWW.

“Bioinformatics” 특집을 내면서

Abstract: BIOE 402. Medical Technology Assessment. 2 or 3 hours. Bioentrepreneur course. Assessment of medical technology in the context of commercialization. Objectives, competition, market share, funding, pricing, manufacturing, growth, and intellectual property; many issues unique to biomedical products. Course Information: 2 undergraduate hours. 3 graduate hours. Prerequisite(s): Junior standing or above and consent of the instructor.

The Role of Root Exudates in Rhizosphere Interactions with Plants and Other Organisms

Abstract: The rhizosphere encompasses the millimeters of soil surrounding a plant root where complex biological and ecological processes occur. This review describes recent advances in elucidating the role of root exudates in interactions between plant roots and other plants, microbes, and nematodes present in the rhizosphere. Evidence indicating that root exudates may take part in the signaling events that initiate the execution of these interactions is also presented. Various positive and negative plant-plant and plant-microbe interactions are highlighted and described from the molecular to the ecosystem scale. Furthermore, methodologies to address these interactions under laboratory conditions are presented.