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H. Harita

Bio: H. Harita is an academic researcher. The author has contributed to research in topics: Gastric mucosa & Omeprazole. The author has an hindex of 2, co-authored 2 publications receiving 31 citations.

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Journal ArticleDOI
TL;DR: The present finding suggests that A. catechu nut extract enhanced ulcer production as ascertained grossly by significant increasing ulcer area, and histologically by comparatively increases in ulcer areas compared to ulcer control group.
Abstract: Areca catechu L. (Arecaceae) nuts have been used in traditional medicine for the treatment of different diseases. The present study was performed to investigate the enhancement of ulcerogenic activity of ethanol extract from nuts of A. catechu in ethanol induced gastric mucosal injury in rats. Four groups of adult Sprague Dawley rats were orally pre-treated respectively with carboxymethyl cellulose (CMC) solution (ulcer control group), omeprazole 20 mg/kg (reference group), 250 and 500 mg/kg A. catechu nut extract in CMC solution (experimental groups) one hour before oral administration of absolute ethanol to generate gastric mucosal injury. After an additional hour, the rats were sacrificed and the ulcer areas of the gastric walls were determined. Grossly, the experimental groups exhibited significantly severe gastric mucosal injury compared to ulcer control group. Histological studies of the gastric wall revealed that experimental groups exhibited comparatively severe damage of gastric mucosa; along with edema and leucocytes infiltration of submucosal layer compared to ulcer control group. In conclusions, the present finding suggests that A. catechu nut extract enhanced ulcer production as ascertained grossly by significant increasing ulcer area, and histologically by comparatively increases in ulcer areas compared to ulcer control group.

22 citations

Journal ArticleDOI
A A Mahmood1, Atieh Abdollahi Fard1, H. Harita, Zahra A. Amin1, I Salmah1 
TL;DR: The data suggest that the ulcer protective activity of S. crispus may be due to its defensive mucin secretion and increase in pH of gastric content, and less mucosal injury, no edema and leucocytes infiltration of submucosa.
Abstract: The anti-ulcerogenic activity of Strobianthes crispus leaf extract was evaluated against ethanol-induced mucosal injury in rats. Five groups of Sprague Dawley rats were pre-treated respectively with: vehicle, distilled water (ulcer control), omeprazole (20 mg kg-1, reference control), 250 mg/kg, 500 mg/kg and 1000 mg/kg S. crispusleaf extracts (experimental groups), 60 min prior to oral administration of absolute ethanol to generate gastric mucosal injury. Sixty minutes later, rats were sacrificed and gastric content, mucus and wall samples collected. Grossly, ulcer control rats exhibited severe injury to the gastric mucosa and decreased gastric mucus content pH of gastric content, whereas rats pre-treated with S. crispus leaf extracts resulted in significantly dose-dependent reduction of gastric lesion formation accompanied by significant increase in gastric mucus production and pH of gastric fluid. Gastric protection was more prominent in 1000 mg/kg of S. crispus-treated group. Histology, ulcer control rats showed the most severe and deepest gastric mucosal necrotic damage, with edema and leukocyte infiltration of the submucosal layer compared to experimental and reference control groups. Thus, our data suggest that the ulcer protective activity of S. crispus may be due to its defensive mucin secretion and increase in pH of gastric content, and less mucosal injury, no edema and leucocytes infiltration of submucosa. Furthermore, acute toxicity study has indicated no mortality with 5 g/kg dose of S. crispus in Sprague Dawley and did not produce any major clinical signs of toxicity. Key words: Anti-ulcer, Strobianthes crispus leaf, acute toxicity, histology.

12 citations


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Journal ArticleDOI
28 May 2013-PLOS ONE
TL;DR: Treatment with the extract led to the upregulation of heat-shock protein 70 (HSP70) and the downregulation of the pro-apoptotic protein BAX and significant increases in the levels of the antioxidant defense enzymes glutathione (GSH) and superoxide dismutase (SOD) in the gastric mucosal homogenate were observed, whereas that of a lipid peroxidation marker (MDA) was significantly decreased.
Abstract: Background: The current study was carried out to examine the gastroprotective effects of Parkia speciosa against ethanolinduced gastric mucosa injury in rats. Methodology/Principal Findings: Sprague Dawley rats were separated into 7 groups. Groups 1–2 were orally challenged with carboxymethylcellulose (CMC); group 3 received 20 mg/kg omeprazole and groups 4–7 received 50, 100, 200 and 400 mg/kg of ethanolic leaf extract, respectively. After 1 h, CMC or absolute ethanol was given orally to groups 2–7. The rats were sacrificed after 1 h. Then, the injuries to the gastric mucosa were estimated through assessment of the gastric wall mucus, the gross appearance of ulcer areas, histology, immunohistochemistry and enzymatic assays. Group 2 exhibited significant mucosal injuries, with reduced gastric wall mucus and severe damage to the gastric mucosa, whereas reductions in mucosal injury were observed for groups 4–7. Groups 3–7 demonstrated a reversal in the decrease in Periodic acid-Schiff (PAS) staining induced by ethanol. No symptoms of toxicity or death were observed during the acute toxicity tests. Conclusion: Treatment with the extract led to the upregulation of heat-shock protein 70 (HSP70) and the downregulation of the pro-apoptotic protein BAX. Significant increases in the levels of the antioxidant defense enzymes glutathione (GSH) and superoxide dismutase (SOD) in the gastric mucosal homogenate were observed, whereas that of a lipid peroxidation marker (MDA) was significantly decreased. Significance was defined as p,0.05 compared to the ulcer control group (Group 2). Citation: Al Batran R, Al-Bayaty F, Jamil Al-Obaidi MM, Abdualkader AM, Hadi HA, et al. (2013) In Vivo Antioxidant and Antiulcer Activity of Parkia speciosa

124 citations

Journal ArticleDOI
TL;DR: Significant increased in the pH, mucus of gastric content and high levels of PGE2, SOD and reduced amount of MDA was observed and the plant promotes ulcer protection as it shows significant reduction of ulcer area grossly.
Abstract: Jasminum sambac is used in folk medicine as the treatment of many diseases. The aim of the present investigation is to evaluate the gastroprotective effects of ethanolic extracts of J. sambac leaves against acidified ethanol-induced gastric ulcers in rats. Seven groups of rats were orally pre-treated with carboxymethylcellulose (CMC) as normal group, CMC as ulcer group, 20 mg/kg of omeprazole as positive group, 62.5, 125, 250, and 500 mg/kg of extract as the experimental groups, respectively. An hour later, CMC was given orally to normal group and acidified ethanol solution was given orally to the ulcer control, positive control, and the experimental groups. The rats were sacrificed after an hour later. Acidity of gastric content, the gastric wall mucus, ulcer areas, and histology and immunohistochemistry of the gastric wall were assessed. Gastric homogenates were determined for prostaglandin E2 (PGE2), superoxide dismutase (SOD), andmalondialdehyde (MDA) content. Ulcer group exhibited significantly severe mucosal injury as compared with omeprazole or extract which shows significant protection towards gastric mucosal injury the plant promotes ulcer protection as it shows significant reduction of ulcer area grossly, and histology showed marked reduction of edema and leucocytes infiltration of submucosal layer compared with ulcer group. Immunohistochemistry showed overexpression of Hsp70 protein and downexpression of Bax protein in rats pretreated with extract. Significant increased in the pH, mucus of gastric content and high levels of PGE2, SOD and reduced amount of MDA was observed.

123 citations

Journal ArticleDOI
TL;DR: In conclusion, oxyresveratrol possess significant anti-ulcer property which might be attributed to attenuated expression levels of IL-6, TNF-α, NF-ĸB, and COX-2 and elevatedexpression levels of TFF-2.

88 citations

Journal ArticleDOI
26 Mar 2015-PLOS ONE
TL;DR: This gastroprotective effect of biochanin A could be attributed to the enhancement of cellular metabolic cycles perceived as an increase in the SOD, NO activity, and decrease in the level of MDA, and also decrease in level of Bax expression and increase the Hsp70 expression level.
Abstract: Background Biochanin A notable bioactive compound which is found in so many traditional medicinal plant. In vivo study was conducted to assess the protective effect of biochanin A on the gastric wall of Spraguedawley rats` stomachs. Methodology The experimental set included different animal groups. Specifically, four groups with gastric mucosal lesions were receiving either a) Ulcer control group treated with absolute ethanol (5 ml/kg), b) 20 mg/kg of omeprazole as reference group, c) 25 of biochanin A, d) 50 mg/kg of biochanin A. Histopathological sectioning followed by immunohistochemistry staining were undertaken to evaluate the influence of the different treatments on gastric wall mucosal layer. The gastric secretions were collected in the form of homogenate and exposed to superoxide dismutase (SOD) and nitric oxide enzyme (NO) and the level of malondialdehyde (MDA) and protein content were measured. Ulceration and patchy haemorrhage were clearly observed by light microscopy. The morphology of the gastric wall as confirmed by immunohistochemistry and fluorescent microscopic observations, exhibited sever deformity with notable thickness, oedematous and complete loss of the mucosal coverage however the biochanin-pretreated animals, similar to the omeprazole-pretreated animals, showed less damage compared to the ulcer control group. Moreover, up-regulation of Hsp70 protein and down-regulation of Bax protein were detected in the biochanin A pre-treated groups and the gastric glandular mucosa was positively stained with Periodic Acid Schiff (PAS) staining and the Leucocytes infiltration was commonly seen. Biochanin A displayed a great increase in SOD and NO levels and decreased the release of MDA. Conclusions This gastroprotective effect of biochanin A could be attributed to the enhancement of cellular metabolic cycles perceived as an increase in the SOD, NO activity, and decrease in the level of MDA, and also decrease in level of Bax expression and increase the Hsp70 expression level.

70 citations

Journal ArticleDOI
TL;DR: In this paper, the authors investigated the gastroprotective effect of Curcuma xanthorrhiza leaf in the rats induced gastric ulcer by ethanol and found that the leaf extract enhanced the gastric protection and sustained the integrity of gastric mucosa structure.
Abstract: Herbal medicines appeared promising in prevention of many diseases. This study was conducted to investigate the gastroprotective effect of Curcuma xanthorrhiza leaf in the rats induced gastric ulcer by ethanol. Normal and ulcer control received carboxymethycellulose (5 mL/kg) orally, positive control was administered with 20 mg/kg omeprazole (reference drug) and 2 groups were received 250 mg/kg and 500 mg/kg of the leaf extract, respectively. To induce of gastric ulcers formation, ethanol (5 mL/kg) was given orally to all groups except normal control. Gross ulcer areas, histology, and amount of prostaglandin E2, superoxide dismutase and malondialdehyde were assessed to determine the potentiality of extract in prevention against gastric ulcers. Oral administration of extract showed significant gastric protection effect as the ulcer areas was remarkably decreased. Histology observation showed less edema and leucocytes infiltration as compared with the ulcer control which exhibited severe gastric mucosa injury. Furthermore, the leaf extract elevated the mucus weight, level of prostaglandin E2 and superoxide dismutase. The extract also reduced malondialdehyde amount significantly. Results showed leaf extract of Curcuma xanthorrhiza can enhanced the gastric protection and sustained the integrity of gastric mucosa structure. Acute toxicity test did not showed any sign of toxicity (2 g/kg and 5 g/kg).

62 citations