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H.J.D. Latupapua

Bio: H.J.D. Latupapua is an academic researcher from Indonesian Institute of Sciences. The author has contributed to research in topics: Microbial inoculant & Nitrogen fixation. The author has an hindex of 1, co-authored 2 publications receiving 16 citations.

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Journal ArticleDOI
TL;DR: The isolation, identification and population of soil microbes from rizosphere at WBiG had been done in the Soil Laboratories Microbiology, Microbiology Division, Research Center of Biology, Indonesian Institute of Sciences (LIPI), Bogor.
Abstract: The isolation, identification and population of soil microbes from rizosphere at WBiG had been done in the Soil Laboratories Microbiology, Microbiology Division, Research Center of Biology, Indonesian Institute of Sciences (LIPI), Bogor. The soil was collected randomly from 16 sites in WBiG, and taken from 0-15 cm depth. Isolates of microbes were identified by Bergeys manual method for bacteria; Ellis method for fungi, and the morphology of isolate method for Actinomycetes. The population of microbes was estimated by plate count method. The result of isolation, identification and population soil microbes from 16 samples in WBiG showed that 20 isolates of bacteria (Azotobactersp., Accinetobacter sp. , Bacillus sp., Citrobacter sp., Flavobacterium sp., Klebsiella sp., Nitrosomonas sp., Pseudomonas sp., Rhizobium sp., Thiobacillus sp., Azospirillum sp., Azotobacter chrococcum, Bacillus panthothenticus, Chromobacterium violaceum, C.lividum,Escherrrichia coli, Flavobacterium breve, Klebsiella aerogenes, Spaerotillus natans, and Staphylococcus epidermidis); nine isolates of fungi (Aspergillus niger, Bisporomyces, Monilia sp., Cephalospharium sp., Verticillum sp., Giocladium sp., Penicillium sp., Nelicocephalum sp., and Cuninghamella sp.), and seven isolates of Actinomycetes (Streptomyces, Streptosporangium, Nocardia, Thermomonospora,Thermoactinomyces, Micromonospora, Mycobacterium). The population of Bacillus (108-109), Rhizobium (106-107), Azospirillum (106-107),and Thiobacillus (104 -109 ) were founded all of soil samples.iƒ“ 2005 Jurusan Biologi FMIPA UNS SurakartaKey words: soil microbes, Wamena Biological Garden (WbiG).

16 citations

DOI
12 Jan 2018
TL;DR: In this article, a green house experiment has been carried out to study the effectiveness of the phosphate solubilizing microbes (PSM) and nitrogen fixing bacteria (NFB) on the growth of maize.
Abstract: The Stimulating Capacities of the Phosphate Solubilizing and the Nitrogen Fixing Microbes on the Maize Plant. The green house experiment has been carried out to study the effectiveness of the phosphate solubilizing microbes (PSM) and nitrogen fixing bacteria (NFB) on the growth of maize (Zea mays). The phosphate solubilizing microbes were Aspergillus sp. and Gigaspora margarita while the nitrogen fixing bacteria were Azospirillum sp. and Azotobacter sp. have been used in this experiment. The aim of the experiment was to find the suitable and effective inoculant on the maize and its expected the growth of the plant. The experiment used The Completely Randomize Design with three replicates. The treatments were uninoculated plant (control), the plants inoculated with single microbe of PSM, NFB and the combination of both. The results indicated that the phosphate solubilizing microbes (PSM) and the nitrogen fixing bacteria (NFB) which were either used in single or combination formula could stimulate the plant growth. The dual inoculation of Azotobacter sp. and Aspergillus sp. were found the higest yield of maize. Key words : The phosphate solubilizing microbes, the nitrogen fixing bacteria, Mycorrhiza vesicular arbuscular, Zea mays.

1 citations


Cited by
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Journal ArticleDOI
TL;DR: In this paper, the authors monitored an illegal sale point in Xalapa, Veracruz, Mexico for 85 weeks to measure the richness and volume of epiphytes that are traded illegally on a local level.

113 citations

BookDOI
01 Jan 2011

103 citations

Journal ArticleDOI
TL;DR: Demographic studies were made in two of its populations in the Sepultura Biosphere Reserve under different conservation conditions, it is proposed that in the nucleus zone, reproductive adults should be of highest conservation priority, whereas in the buffer zone seedling reintroduction should be carried out regularly until the population increases.
Abstract: The cycad Ceratozamia mirandae is endemic to Chiapas, Mexico. Demographic studies were made in two of its populations in the Sepultura Biosphere Reserve under different conservation con- ditions; in the nucleus zone ''Tres Picos'' (conserved) and buffer zone ''La Sombra'' (disturbed and under management). Spatial distribution of C. mirandae was aggregated, showed a clumped local distribution on shallow soils on steep slopes and male and female cones appear to be synchronous in both populations. The population structure was of type I (Bongers) for both sites. Individuals between the sites showed dif- ferences in growth pattern. The oldest plants (80- 90 cm tall) were estimated to be about 490 years at ''La Sombra''. The finite growth rate (k) in the buffer zone population showed a tendency for decrease whilst in the nucleus zone this estimate remained stable. The highest elasticity values lied in the transition of the first three classes of the ''La Sombra'' population, in ''Tres Picos'' this corresponded to adult plants between 20 and 30 cm tall. Given the above, it is proposed that in the nucleus zone, reproductive adults should be of highest conservation priority, whereas in the buffer zone seedling reintroduction should be carried out regularly until the population increases. We recom- mend an IUCN Red List category of Vulnerable (VU C, 2a), largely due to difficult-to-control destructive annual forest fires that occur in this Reserve.

54 citations

Journal ArticleDOI
TL;DR: The presence of phosphate-solubilizing microorganisms and their ability to solubilize phosphate were indicative of the important role played by bacteria in the biogeochemical cycle of phosphorus and the plant growth in coastal ecosystems.
Abstract: The occurrence and distribution of an actinobacteria group of bacteria capable of dissolving insoluble phosphates were investigated in this study in marine environments, especially in sediments of Chorao Island, Goa Province, India. A total of 200 bacterial isolates of actinobacteria was isolated. All isolates were screened for phosphate-solubilizing activity on Pikovskaya’s agar. Thirteen different isolates exhibiting maximum formation of halos (zone of solubilization) around the bacterial colonies were selected for quantitative estimations of P-solubilization. Quantitative estimations for P-solubilization were analyzed for up to 10 days at intervals of 24 h. Maximum solubilization from 89.3 ± 3.1 to 164.1 ± 4.1 μg ml−1 was observed after 6 days of incubation in six of all isolates, while the isolate NII-1020 showed maximum P-solubilization. The increase in solubilization coincided with the drop in pH. Many of these species showed wide range of tolerance to temperature, pH, and salt concentrations. Further, 16S rRNA gene sequence analyses were carried to identify the bacterial groups which are actively solubilized phosphate in vitro. Gene sequencing results reveal that all isolates were clustered into six different actinobacterial genera: Streptomyces, Microbacterium, Angustibacter, Kocuria, Isoptericola, and Agromyces. The presence of phosphate-solubilizing microorganisms and their ability to solubilize phosphate were indicative of the important role played by bacteria in the biogeochemical cycle of phosphorus and the plant growth in coastal ecosystems.

43 citations

Journal Article
TL;DR: The results suggest that the high microbial diversity index, the low population density of Foc, and the high population of antagonistic microbes in the soil of the banana habitat without Fusarium wilt symptom were able to suppress the development of Fusaria wilt disease on banana.
Abstract: Fusarium oxysporum f.sp. cubense (Foc), the cause of the Fusarium wilt disease on banana plant, is one of the important soil borne pathogens which may lead to a significant loss of banana yield in Indonesia. This study was done in order to know the soil’s microbial diversity in the banana habitat with and without Fusarium wilt symptom. The soil samples were collected from three regencies in Bali, i.e. Karangasem, Klungkung and Jembrana which are the main banana growing areas in Bali. Soil sampling was done in two sites in each regency representing the banana habitat with and without Fusarium wilt symptom, by collecting 100 grams of soil surrounding the banana plant at the depth of 20 cm. Soil microbes population density particularly for bacteria, fungi and actinomycetes were determined based on plate count technique, while the microbial diversity was determined based on the Diversity Index of Shannon& Wiener. Results of the present study showed that the density of soil microbes in the soil of banana habitat without Fusarium wilt symptom (HN) was 13.85 x 10 6 cfu per gram of soil, which was significantly higher than 1.02 cfu per g of soil of banana habitat with Fusarium wilt symptom (HF), while the density of Foc in the soil of HN is 0.003 x 10 6 cfu per g of soil, which is lower than 0.01 x 10 6 cfu per g of soil of HF. The diversity index of the soil microbes in the soil of HN was 2.03, which was higher than 1.91 of HF. The domination index of the soil microbes in the soil of HN was 0.81, which was higher than 0.78 of HF, wherein Bacillus spp. and Pseudomonas spp. were the dominant microbes. The density of soil microbes which are potentially antagonistic against Foc, such as Bacillus spp., Streptomyces spp., Trichoderma spp., Aspergillus spp., Penicillium spp. and Gliocladium sp. is higher in the soil of HN compared to the soil of HF. Among the antagonistic microbes, Bacillus sp. showed the highest inhibitory activity against Foc. These results suggest that the high microbial diversity index, the low population density of Foc, and the high population of antagonistic microbes in the soil of the banana habitat without Fusarium symptom were able to suppress the development of Fusarium wilt disease on banana.

20 citations