Author
H. Schwander
Bio: H. Schwander is an academic researcher from University of Bern. The author has an hindex of 2, co-authored 2 publications receiving 130 citations.
Papers
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TL;DR: In this article, eine Abanderung der bekannten Verfahren zur Isolierung von Desoxyribonucleinsaure angegeben is discussed.
Abstract: Es wird eine Abanderung der bekannten Verfahren zur Isolierung von Desoxyribonucleinsaure angegeben. Die so hergestellten Praparate zeichnen sich durch hohes Molekulargewicht aus. Die Nucleinsaure wird gekennzeichnet durch Viskositat, Stromungsdoppelbrechung und Trubungsgrad verdunnter Losungen und durch die Saure- und Basenbindung.
73 citations
TL;DR: In this article, a Verfahren beschrieben, das die Darstellung hochmolekularen Natrium-thymonucleinats in grosseren Mengen with guter Ausbeute gestattet.
Abstract: Es wird ein Verfahren beschrieben, das die Darstellung hochmolekularen Natrium-thymonucleinats in grosseren Mengen mit guter Ausbeute gestattet. Nach verschiedenen Methoden hergestellte Praparate werden auf Proteingehalt und Viskositaten ihrer verdunnten Losungen untersucht.
57 citations
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TL;DR: Evidence is presented that formation of this complex (complex I) is specific for base-paired regions either in DNA, RNA or RNA: DNA hybrids, and that the basis of this specificity is intercalation of the dye between base pairs.
2,229 citations
TL;DR: A new spectrofluorometric method for determining DNA and RNA using this principle is described, specific for nucleic acids, can be used over a wide range of salt concentrations, and has a good sensitivity.
643 citations
TL;DR: New and published data are analyzed to show that light-scattering determinations of molecular weight are uniformly reliable only below 3 million, and that the value of the Scheraga-Mandelkern constant β evidently remains very close to 2·4 × 10 6 throughout the molecular weight range of 200,000 to 200 million.
508 citations
TL;DR: This chapter presents the preparation for extraction of DNA form thymus gland, spleen and fowl/fish erythrocytes, and the use of a Waring blendor or other stainless steel disintegrators is avoided.
Abstract: Publisher Summary This chapter presents the preparation for extraction of DNA form thymus gland, spleen and fowl/fish erythrocytes. All the steps from thawing the glands until the first alcohol precipitation are performed as fast as possible; otherwise, owing to the action of thymus DNase, a product is obtained which can be shown to be degraded. All the steps are performed at 0 to 4 °, with glass, rubber, or plastic tools and vessels. In particular, minute traces of rust cause rapid degradation;for this reason the use of a Waring blendor or other stainless steel disintegrators is avoided. While extracting DNA from the calf spleen the finally deproteinized material is redissolved in standard buffer to yield a clear, very viscous solution containing 1 to 1.5 mg. of DNA/per milliliter. Total yield, 100 to 300 mg. For DNA from chicken erythrocytes, cells are centrifuged with sodium citrate solution. The cells are broken by freezing and thawing, are stirred in 60 ml. The homogenate obtained is then centrifuged for 1 hour at 1800 X g, and the supernatant discarded. The sediment is resuspended in 1 vol. of the same citrate solution, again centrifuged as above, and the supernatant is discarded. The sediment is then extracted with 400 ml. of 2 M NaC1 solution.
413 citations
TL;DR: In this paper, an analysis has been made of the oriented crystalline pattern given by fibres of the lithium salt (LiDNA) at 66% relative humidity, which is known as the B type and exists in unfixed cell nuclei.
384 citations