H. V. Kolisnyk

Bio: H. V. Kolisnyk is an academic researcher. The author has contributed to research in topics: Agriculture & Environmentally friendly. The author has an hindex of 1, co-authored 1 publications receiving 2 citations.

Reduction of chromate and carotene-synthesizing activity of selenite-resistant mutants of the yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma)

Abstract: Aim. The yeast P. rhodozymà is a perspective microbial producer of carotenoid pigment astaxanthin with a high antioxidant power. The aim of the work was to study the ability of the selenite-resistant strains of this yeast to reduce chrome(VI) compounds, as well as to analyze the relations between synthesis of carotenoids, resistance to selenite and chromate-reducing activity of P. rhodozymà. Methods. The yeast cells were grown at standard conditions for this species. The residual chromate content in cultural liquid was determined colorimetrically using diphenylcarbazide. The carotenoid content was determined after extraction of the pigments from the previously permeabilized cells by organic solvents. Results. The selected selenite-resistant mutants of the yeast P. rhodozymà revealed the different combinations of the phenotypes related with tolerance/sensitivity to chromate and selenite, as well as ability to reduce chromate. Conclusions. The obtained results give reasons for suggesting that pathways of detoxification of chromate and selenite by the yeast P. rhodozyma are different, although run through a common reductive type. The isolated mutant strains would be served as the useful models to study relations between homeostasis of Se and Cr oxyanions and biosynthesis of carotenes.

Gis Tools in the Formation of Environmentally Friendly Use of Agricultural Landscapes

Abstract: SUMMARY The report presents the methodology for determining ecological and technological limitations in the use of agricultural landscapes using GIS tools and analyses it on the example of agricultural landscapes of the Vinnytsia Municipal Territorial Community (MTC). It is proved, that digital relief model generated with GIS tools can be used to plan and form environmentally friendly agricultural landscape use with high accuracy and efficiency.

The kinetic reduction of Cr(VI) by yeast Saccharomyces cerevisiae, Phaffia rhodozyma and their protoplasts.

Abstract: Chromium in the sixth oxidation state may easily penetrate cellular membranes via non-specific sulfate transporters due to its tetrahedral symmetry (high similarity to SO4(2-) and HPO4(2-)). This feature makes chromium a toxic and hazardous pollutant responsible for the deterioration of midland water quality. The aim of the study was to evaluate the capacity of two yeast species - Saccharomyces cerevisiae and Phaffia rhodozyma - and their protoplasts to reduce Cr(VI) to lower oxidation states. The study also deals with the behavior of the yeasts upon the presence of elevated sulfate ions as a competitive inhibitor of chromate transport by the sulfate transporters. The chromate-reducing activities were monitored by determination of Cr(V) free radical form with the use of L-band (1.2 GHz) EPR (electron paramagnetic resonance) spectroscopy. It was observed that both of the studied yeast strains exhibited the ability to reduce Cr(VI) applied at 4 mM. The cells of P. rhodozyma showed about 3.5 times higher reduction than S. cerevisiae. The reduction efficiency was significantly improved when the protoplasts of both strains were used and reached 100% in the first 10 minutes of the reduction process which suggests that the cellular wall may have a notable influence on the uptake and/or inhibition of chromium reduction process. The reduction effect of P. rhodozyma cells and protoplasts may be associated with the more sufficient production of metabolites (such as glutathione and cysteine), which may also be responsible for the increased tolerance of the strain towards high concentrations of toxic chromium.

Chromate-resistant mutants of the yeast Pichia guilliermondii: Selection and properties

Abstract: Chromate-resistant mutants of the non-conventional yeast Pichia guilliermondii L2 were selected by different methods. The isolated mutants were capable of better growth and higher biomass yield at toxic (1.8–2.4 mM) chromate concentrations than the parent strain. The capacity of the mutants for extracellular chromate reduction and chelation of Cr(III) in the culture liquid was demonstrated. The effectiveness of these processes was shown to correlate with the resistance of P. guilliermondii strains to chromate. Extracellular metabolites of the yeast cells cultivated without chromate were shown to be capable of reducing chromate and forming stable soluble Cr(III)-biocomplexes.

The issue of the display of sensitive information in the state land cadastre and its publicity in the conditions military conflict

Abstract: The goal of the scientific work was to justify the need during the war in Ukraine to find a balance in the issue of limiting access to sensitive in terms of state and public security open data in the State Land Cadastre system to ensure the interests of national security and public interests. Information of interest to the aggressor should become inaccessible. This data selection should be made by the military and the Security Service and should not be publicly displayed. On the other hand – the rest of the information should be used by specialists in the field of geodesy and land management, construction and architecture, local self-government bodies with a certain number of persons with access to data. The author's proposal is also that access to data, including open data, should be regulated and authorized. So it is proposed to classify them at the legislative level in terms of levels of sensitivity, methods of use, access regulations, and to define lists of sensitive information and ways of displaying it. The need to change the attitude towards information resources as free resources and transfer information to the status of an intangible asset with licensed access (analogue – software) was noted, because the introduction of information into economic circulation will cause the growth of the economy. It is proposed that upon the end of martial law, the restrictions that were in force under these conditions should be reviewed, amendments to the legislation should be introduced into the regulations of the State Land Cadastre, and the composition, content, and distribution capacity of the cartographic base of the Public Cadastre Map should be updated.