H
Hannes Schihada
Researcher at Karolinska Institutet
Publications - 18
Citations - 166
Hannes Schihada is an academic researcher from Karolinska Institutet. The author has contributed to research in topics: Frizzled & G protein-coupled receptor. The author has an hindex of 5, co-authored 13 publications receiving 48 citations. Previous affiliations of Hannes Schihada include University of Würzburg.
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Journal ArticleDOI
Development of a Conformational Histamine H3 Receptor Biosensor for the Synchronous Screening of Agonists and Inverse Agonists.
Hannes Schihada,Hannes Schihada,Xiaoyuan Ma,Ulrike Zabel,Henry F. Vischer,Gunnar Schulte,Rob Leurs,Steffen Pockes,Martin J. Lohse,Martin J. Lohse +9 more
TL;DR: The design and validation of the first screening-compatible H3R conformational biosensor that will aid in the discovery of novel H3 R ligands and can be employed to gain deeper insights into the (in-)activation mechanism of this highly attractive drug target.
Journal ArticleDOI
eGFP-tagged Wnt-3a enables functional analysis of Wnt trafficking and signaling and kinetic assessment of Wnt binding to full-length Frizzled
Janine Wesslowski,Paweł Kozielewicz,Xianxian Wang,Haijun Cui,Hannes Schihada,Dominique Kranz,Pradhipa Karuna M,Pavel A. Levkin,Julia Christina Gross,Michael Boutros,Gunnar Schulte,Gary Davidson +11 more
TL;DR: The development of a biologically active, fluorescent Wnt-3a reported here opens up the technical possibilities to unravel the intricate biology of Wnt signaling and WNT-receptor selectivity.
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Quantitative assessment of constitutive G protein-coupled receptor activity with BRET-based G protein biosensors.
TL;DR: In this paper, the primary transducers of G protein-coupled receptor (GPCR) signaling were identified as heterotrimeric G proteins, which constitute the primary signal transducers.
Journal ArticleDOI
A Versatile Sub-Nanomolar Fluorescent Ligand Enables NanoBRET Binding Studies and Single-Molecule Microscopy at the Histamine H 3 Receptor
Niklas Rosier,Lukas Grätz,Hannes Schihada,Jan Möller,Jan Möller,Ali Işbilir,Ali Işbilir,Laura J Humphrys,Martin Nagl,Ulla Seibel,Martin J. Lohse,Martin J. Lohse,Steffen Pockes,Steffen Pockes +13 more
TL;DR: In this paper, a novel fluorescent H3R ligand called UR-NR266 was synthesized, which showed fast association to its target and complete dissociation in kinetic binding studies.
Journal ArticleDOI
Deconvolution of WNT-induced Frizzled conformational dynamics with fluorescent biosensors.
TL;DR: In this paper, the authors developed fluorescence-based biosensors that detect WNT-induced FZD conformational changes in living cells in order to assess WNT action via FZDs at the most proximal level, i.e. the receptor conformation.