Hasibe Cingilli Vural

Bio: Hasibe Cingilli Vural is an academic researcher from Selçuk University. The author has contributed to research in topics: DNA extraction & Apoptosis. The author has an hindex of 8, co-authored 27 publications receiving 153 citations.

Optimization of DNA isolation for RAPD-PCR analysis of selected (Echinaceae purpurea L. Moench) medicinal plants of conservation concern from Turkey.

Abstract: Genetic analysis of plants relies on high yields of pure DNA samples. Here we present the optimization of DNA isolation and PCR conditions for RAPD analysis of selected medicinal plants of conservation concern from Turkey, containing high levels of polysaccharides, polyphenols and secondary metabolites. The method involves a modified CTAB extraction employing polyvinyl pyrrolidone (PVP) while grinding, successive long-term chloroform-isoamylalcohol extractions, EZ1 nucleic acid isolation protocols. The yield of DNA ranged from 1 - 2 μg/μl per gram of the leaf tissue and the purity (ratio) was between 1.7 - 1.8 indicating minimal levels of contaminating metabolites. EZ1 nucleic acid isolation technique is ideal for isolation of DNA from different plant species and the DNA isolated was used for randomly amplified polymorphic DNA (RAPD) analysis. RAPD protocol was optimized based on the use of higher concentration of MgCl2 (3 mM), lower concentrations of primer (0.5 μM) and Taq polymerase (0.2 units), 50 ng of template DNA and an annealing temperature of 37°C, resulted optimal amplification. Reproducible amplifiable products were observed in all PCR reactions. Thus, the results indicate that the optimized protocol for DNA isolation and PCR was amenable to plant species belonging to different genera which is suitable for further work on diversity analysis. Furthermore, here we used suitable DNA isolation protocol for RAPD analysis to study the genetic variation in the future in Echinaceae sp. grown in Turkey. Key words: Echinaceae purpurea, random amplified polymorphic DNA, polymerase chain reaction, medicinal plant.

Antioxidant activity and fatty acid composition of ginkgo biloba from turkey

Abstract: In this study, we investigated the antioxidant capacity and total phenolic content of the extracts Ginkgo biloba from Turkey. The antioxidant activity of the methanolic and acetone extracts from G. biloba leaves was measured by various assays, including ferric reducing antioxidant power assay, cupric reducing antioxidant capacity assay and metal chelating capacity. Total phenolic content of the extracts was measured as gallic acid equivalents (GAE) by Folin–Ciocalteu reagent. The methanolic extract showed higher antioxidant activity related to high phenolic content with 76.0 ± 5.2 mg GAE/g dry weight. Fatty acid compositions of the methanolic and acetone extracts of G. biloba were analyzed. Data suggested that G. biloba grown in Turkey may be an important source of natural antioxidant. PRACTICAL APPLICATIONS This study reveals antioxidant activity of the methanolic and acetone extracts of Ginkgo biloba leaves growing in Turkey measured by various assays, including ferric reducing antioxidant power assay, cupric reducing antioxidant capacity assay and metal chelating capacity. Total phenolic content of the extracts was also measured as gallic acid equivalents by Folin–Ciocalteu reagent. Fatty acid compositions of the extracts of G. biloba were analyzed by gas chromatography.

Radioactivity concentrations and dose assessments of therapeutic peloids from some Turkish spas

Abstract: The activity concentrations of natural radionuclides in peloids were studied to assess the radiologic hazard from 18 Turkish spas. The peloids are mainly used for therapeutic treatments, rheumatic diseases and aesthetic purposes. The concentrations of the natural radionuclides 226Ra, 232Th, 40K and 137Cs were determined with a gamma ray spectrometer using a HPGe detector. The average activity concentrations of 226Ra, 232Th, 40K, and 137Cs in the peloids studied were 110.69, 71.52, 576.48 and 0.447 Bq/kg, respectively. The radium equivalent activities in the peloid samples ranged from 63.3 to 766.77 Bq/kg. The absorbed dose rate (Dout) varied between 37.52 and 330.67 nGy/h and most of the observed spa doses are greater than the worldwide recommended values. The annual effective dose values range from 0.26 to 2.78 μSv/y. The annual gonadal dose equivalents of the samples vary from 224.07 to 2283.55 with a mean of 821.99 μSv/y.

Biochemical and molecular analysis of soybean seed from Turkey

Abstract: We aimed molecular analysis of soybean by lecithin specific primer pairs LE5/LE6. Genomic DNA was extracted from soybean by CTAB method and EZ1 nucleic acid isolation system. A sensitive qualitative detection method for soybean, using the polymerase chain reaction was developed with E5/LE6 primers, produced a 195 bp product. However, the antioxidant activity of methanolic extract of soybean from Turkey by using DPPH and ABTS radical scavenging assays, showed higher antioxidant activity with 53.19±0.87% and 45.10±0.32%, respectively. Fatty acid compositions and several phenolic acids and flavonoids of soybean extract were analysed by gas and high performenace chromatography. Data showed that, the main compounds of the extract were eriodictyol, naringenin and linoleic acid. Key words: Antioxidant activity, fatty acid, flavonoid, soybean, molecular marker.

Modern Nutrition in Health and Disease

Abstract: This 11th edition of the book Modern Nutrition in Health and Disease, featuring the work of more than 190 expert authors and divided into five parts, fully explains and encapsulates the fundamentals of nutrition and its role in contemporary society, from mastering the basic science of nutrient metabolism and function to applying nutritional concepts to combat human disease. Part I comprehensively covers specific dietary components, including major dietary constituents, minerals, vitamins and other Other CABI sites 

Phytochemicals and uses of Moringa oleifera leaves in Ugandan rural communities.

Abstract: Moringa oleifera grown and used in many countries around the world is a multi-purpose tree with medicinal, nutritional and socio-economic values. In Senegal and Benin, M. oleifera leaves are dispensed as powder at health facilities to treat moderate malnutrition in children. It established the medicinal uses of M. oleiferaleaves by local communities in Uganda and identified phytochemicals present inM. oleifera leaves extracts. It used quantitative and experimental methods that established the uses, and identified phytochemicals in M. oleifera leaves. Employed serial extractions, using ether, ethanol and water as solvents. The phytochemicals were qualitatively identified using standard chemicals and standard outcomes. Twenty-four medicinal uses of M. oleifera leaves were established. Phytochemicals present included: tannins, steroids and triterponoids, flavonoids, saponins, anthraquinones, alkaloids and reducing sugars. The local communities in Uganda use M. oleifera leaves to treat common ailments. Presence of phytochemicals in the extracts, indicate possible preventive and curative property of M. oleifera leaves. There is need to standardize M. oleifera leaves use for nutrition and herbal medicine. Key words: Phytochemicals, medicinal plant extracts, herbal medicine, Uganda.

Antioxidant activity and protecting health effects of common medicinal plants.

Abstract: Medicinal plants are traditionally used in folk medicine as natural healing remedies with therapeutic effects such as prevention of cardiovascular diseases, inflammation disorders, or reducing the risk of cancer. In addition, pharmacological industry utilizes medicinal plants due to the presence of active chemical substances as agents for drug synthesis. They are valuable also for food and cosmetic industry as additives, due to their preservative effects because of the presence of antioxidants and antimicrobial constituents. To commonly used medicinal plants with antioxidant activity known worldwide belong plants from several families, especially Lamiaceae (rosemary, sage, oregano, marjoram, basil, thyme, mints, balm), Apiaceae (cumin, fennel, caraway), and Zingiberaceae (turmeric, ginger). The antioxidant properties of medicinal plants depend on the plant, its variety, environmental conditions, climatic and seasonal variations, geographical regions of growth, degree of ripeness, growing practices, and many other factors such as postharvest treatment and processing. In addition, composition and concentration of present antioxidants, such as phenolic compounds, are related to antioxidant effect. For appropriate determination of antioxidant capacity, the extraction technique, its conditions, solvent used, and particular assay methodology are important.