Heike Kunze-Schumacher

TL;DR: These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community providing the theory and key practical aspects offlow cytometry enabling immunologists to avoid the common errors that often undermine immunological data.

TL;DR: Temporal analysis of development using a molecular timer in combination with loss of miR‐181a/b‐1 revealed thatMAIT cells complete functional maturation in the periphery and indicates that functionally mature MAIT cells in the thymus are long‐term resident cells, providing insight into the dynamics of MAIT cell development in vivo.

TL;DR: It is proposed that intrathymic miR-181a/b-1 controls development of Treg cells and imposes a developmental legacy on their peripheral function.

TL;DR: In this article, the authors review experimental strategies aimed at revealing quantitative and dynamic properties of T-cell development and how they have been implemented in mathematical modeling strategies that were reported to help understand the flexible dynamics of the highly dividing and dying thymic cell populations.

TL;DR: The role of miRNAs in Treg cell development and function is reviewed and the question what the study of mi RNAs in this context reveals about miRNA biology in general is focused on, including context-dependent function and the role of individual targets vs. complex co-targeting networks.