scispace - formally typeset
Search or ask a question
Author

Henni Kallak

Bio: Henni Kallak is an academic researcher from University of Tartu. The author has contributed to research in topics: Kinetin & Explant culture. The author has an hindex of 3, co-authored 3 publications receiving 41 citations.

Papers
More filters
Journal ArticleDOI
TL;DR: C caulogenesis essentially depended on explant source: internodal calli of all the tested cultivars practically missed the shoot formation ability, and the number of caulogenetic calli from apical-nodal segments significantly depended on genotype, but was also affected by growth regulators.
Abstract: For callus induction, shoot tips and nodal or internodal stem segments of carnation cultivars (Coral, Jaguar, Salome and Sarinah) were grown on MS basal medium with 2,4-dichlorophenoxyacetic acid and kinetin. To achieve organogenesis, calli were transferred onto MS medium without or with growth regulators (indoleacetic acid, naphthaleneacetic acid, indolebutyric acid, kinetin, benzyladenine) in different combinations. Shoot primordia emerged from the subsurface meristemoids of calli, roots developed from the inner callus cells. The effects of genotype, explant source and growth regulators on callus-mediated organogenesis differently manifested themselves in caulogenesis and rhizogenesis, respectively. The number of root-forming calli most of all depended on genotype and least of all on explant source. Unlike rhizogenesis, caulogenesis essentially depended on explant source: internodal calli of all the tested cultivars practically missed the shoot formation ability. The number of caulogenetic calli from apical-nodal segments significantly depended on genotype, but was also affected by growth regulators.

27 citations

Journal ArticleDOI
TL;DR: Variations of morphogenetic behaviour observed in meristem cultures of the investigated pea cultivars manifested a rather strict genetic determination of their regeneration abilities, especially in respect of flowering and rooting of the regenerated shoots.

9 citations

Journal ArticleDOI
TL;DR: Significant differences among genotypes in shoot formation and growth efficiency, as well as in the rate of abnormality, rooting and callusing of the shoots were observed, and the morphogenetic responses were also affected by the growth regulators.

6 citations


Cited by
More filters
Book
11 Dec 2000
TL;DR: Chemistry nutrition plant physiology, agronomy processing biotechnology breeding strategies for improving grain legume carbohydrates, and research into breeding strategies to improve grain legumes carbohydrates are presented.
Abstract: Chemistry nutrition plant physiology and agronomy processing biotechnology breeding strategies for improving grain legume carbohydrates. (Part contents).

110 citations

Book ChapterDOI
01 Jan 1994
TL;DR: Among the prevalent tissue culture techniques of agricultural and horticultural promise, meristem and shoot tip culture has been exploited at a much wider scale primarily due to its application in diverse areas such as rapid clonal multiplication of vegetatively propagated crop plants, virus elimination and germplasm preservation.
Abstract: Among the prevalent tissue culture techniques of agricultural and horticultural promise, meristem and shoot tip culture has been exploited at a much wider scale primarily due to its application in diverse areas such as rapid clonal multiplication of vegetatively propagated crop plants, virus elimination and germplasm preservation of both vegetatively and seed propagated crops (Kartha, 1981; 1986; George and Sherrington, 1984; Hussey, 1986), and more recently in the development of genetic transformation protocols for crop improvement through gene transfer (Ulian, 1988; Gould et al., 1991).

59 citations

Journal ArticleDOI
TL;DR: An in vitro propagation system was developed for castor-bean through cotyledon derived callus cultures and the impact of different concentrations of auxins, cytokinins, additives, amino acids and sugars were evaluated for callus induction and shoot proliferation.
Abstract: An in vitro propagation system was developed for castor-bean (Ricinus communis L. cv. TMV 6) through cotyledon derived callus cultures. The impact of different concentrations of auxins, cytokinins, additives, amino acids and sugars were evaluated for callus induction and shoot proliferation. Green compact nodular organogenic callus was obtained on the medium fortified with Murashige and Skoog (MS) salts, B5 vitamins, 2.0 mg dm−3 6-benzyladenine and 0.8 mg dm−3 α-naphthalene acetic acid (NAA). Multiple shoot proliferation from the callus cultures was achieved on the medium with MS salts, B5 vitamins, 2.5 mg dm−3 thidiazuron (TDZ), 0.4 mg dm−3 NAA and 15 mg dm−3 glutamine. During multiple shoot induction the phenolic secretion was controlled by the addition of 15 mg dm−3 polyvinylpyrolidone. The proliferated shoots were elongated on the medium comprising MS salts, B5 vitamins, 1.5 mg dm−3 TDZ and 0.3 mg dm−3 gibberellic acid. The elongated shoots were rooted on the medium containing MS salts, B5 vitamins, 0.3 mg dm−3 indole-3-butyric acid and 0.6 mg dm−3 silver nitrate. After root induction, the plants were hardened in earthen pots containing sand, soil and vermiculite.

58 citations

Journal ArticleDOI
TL;DR: The use of kanamycin and timentin with or without carbenicillin, was very useful in the transformation procedure, for the elimination of Agrobacterium in regenerated shoots before their transfer to greenhouse conditions and also in the selection of transgenic versus false-positive shoots.
Abstract: The effect of several β-lactam antibiotics on shoot regeneration, growth and rooting of carnation (Dianthus caryophyllus L.), and their use in combination with kanamycin in Agrobacterium-mediated genetic transformation studies, was determined. Carbenicillin, cefotaxime and ticarcillin increased the regeneration rate when added alone in non-inoculated explants; whereas, with inoculated explants, this effect was only observed in ticarcillin-containing medium. Cefotaxime inhibited root growth in both transgenic and non-transgenic shoots. Rooting of non-transgenic shoots was completely inhibited in all culture media containing kanamycin. The different antibiotics used, alone or in combination, did not prevent the occurrence of false positive shoots, but it was possible to select transgenic shoots when rooting was induced in a kanamycin + ticarcillin-containing medium. Regenerated transformed shoots were free of Agrobacterium after culturing in rooting medium, as was proven by the PCR analysis for the nptI gene, the antibiogram and the culture of tissue pieces of transgenic shoots on LB broth. The use of kanamycin and timentin with or without carbenicillin, was very useful in the transformation procedure, for the elimination of Agrobacterium in regenerated shoots before their transfer to greenhouse conditions and also in the selection of transgenic versus false-positive shoots.

44 citations

Journal ArticleDOI
TL;DR: The capacity for indirect shoot organogenesis of leaf and root explants of four Dieffenbachia cultivars were examined on a modified Murashige and Skoog medium supplemented with different plant growth regulators in 112 combinations and significant differences in callus and shoot formation from leaf explants were also observed among cultivars.
Abstract: The capacity for indirect shoot organogenesis of leaf and root explants of four Dieffenbachia cultivars were examined on a modified Murashige and Skoog (MS; Physiol Plant 15:473–495, 1962) medium supplemented with different plant growth regulators in 112 combinations. Callus formation was only observed from leaf explants on MS supplemented with 1–10 μM thidiazuron (TDZ) and 0.5–1.0 μM 2,4-dichlorophenoxyacetic acid (2,4-D) regardless of cultivars. The combination of 5 μM TDZ and 1 μM 2,4-D resulted in the greatest callus formation frequency among the four cultivars tested. Significant differences in callus and shoot formation from leaf explants were also observed among cultivars. Cultivars Camouflage, Camille, Octopus, and Star Bright produced green nodular, brown nodular, yellow friable, and green compact calli with corresponding maximum callus formation frequencies of 96%, 62%, 54%, and 52%, respectively. A maximum of 6.7 shoots/callus was observed in cv. Camouflage, followed by cvs. Camille and Star Bright at 3.7 and 3.5, respectively. Calli of cv. Octopus displayed no capacity for shoot organogenesis. Regardless of cultivar, callus formation was not observed on root explants. Regenerated shoots were successfully acclimatized in a shaded greenhouse condition with 100% survival.

34 citations