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Hiroshi Sugiyama

Bio: Hiroshi Sugiyama is an academic researcher from Kyoto University. The author has contributed to research in topics: DNA & DNA origami. The author has an hindex of 67, co-authored 743 publications receiving 20128 citations. Previous affiliations of Hiroshi Sugiyama include Scripps Research Institute & University of Tokyo.
Topics: DNA, DNA origami, Medicine, Base pair, Oligonucleotide


Papers
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Journal ArticleDOI
TL;DR: In this paper, molecular orbital calculations of stacked DNA bases were performed at 3-21G and 6-31G levels to elucidate the origin of the 5'-GG-3' sequence specificity for the photocleavage of DNA in the presence of electron-accepting photosensitizers.
Abstract: Ab initio molecular orbital calculations of stacked DNA bases were performed at the 3-21G(*) and 6-31G* levels to elucidate the origin of the 5‘-GG-3‘ sequence specificity for the photocleavage of DNA in the presence of electron-accepting photosensitizers. Ionization potentials (IP) were estimated as Koopman's theorem values for 16 sets of two stacked nucleobases and seven sets of stacked nucleobase pair systems in a B-form geometry. It was found that the GG/CC system is the lowest among the 10 possible stacked nucleobase pairs and that approximately 70% of the HOMO is localized on the 5‘-G of 5‘-GG-3‘. These calculations indicate that the 5‘-G of 5‘-GG-3‘ is the most electron donating site in B DNA and suggest that one-electron transfer from DNA to an electron acceptor occurs most effectively at 5‘-GG-3‘ sites which are fully consistent with the experimental data. In order to know the fate of the cation radical, the vertical IPs were estimated for seven stacked nucleobase pairs. It was found that the GG/...

568 citations

Journal ArticleDOI
TL;DR: The results suggest that the 22 nt in K(+) solution exists as a mixture of mixed-parallel/antiparallel and chair-type G-quadruplex, and the possible implications of the structure in understanding higher-order telomeric DNA structure and T-loop formation are discussed.

344 citations

Journal ArticleDOI
TL;DR: It is shown that the path of a motor through a network of tracks containing four possible routes can be programmed using instructions that are added externally or carried by the motor itself, and when external control is used it is found that 87% of the motors follow the correct path.
Abstract: Synthetic molecular motors can be fuelled by the hydrolysis or hybridization of DNA. Such motors can move autonomously and programmably, and long-range transport has been observed on linear tracks. It has also been shown that DNA systems can compute. Here, we report a synthetic DNA-based system that integrates long-range transport and information processing. We show that the path of a motor through a network of tracks containing four possible routes can be programmed using instructions that are added externally or carried by the motor itself. When external control is used we find that 87% of the motors follow the correct path, and when internal control is used 71% of the motors follow the correct path. Programmable motion will allow the development of computing networks, molecular systems that can sort and process cargoes according to instructions that they carry, and assembly lines that can be reconfigured dynamically in response to changing demands.

338 citations

Journal ArticleDOI
TL;DR: This work assembles a 100-nm-long DNA track on a two-dimensional scaffold, and shows that a DNA motor loaded at one end of the track moves autonomously and at a constant average speed along the full length of theTrack, a journey comprising 16 consecutive steps for the motor.
Abstract: Real-time atomic force microscopy can be used image the individual steps of a DNA motor as it moves, autonomously and at constant average speed, along a 100-nm track.

331 citations


Cited by
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28 Jul 2005
TL;DR: PfPMP1)与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用,在黏附及免疫逃避中起关键的作�ly.
Abstract: 抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1(PfPMP1)与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用,在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员,通过启动转录不同的var基因变异体为抗原变异提供了分子基础。

18,940 citations

Journal ArticleDOI
01 Apr 1988-Nature
TL;DR: In this paper, a sedimentological core and petrographic characterisation of samples from eleven boreholes from the Lower Carboniferous of Bowland Basin (Northwest England) is presented.
Abstract: Deposits of clastic carbonate-dominated (calciclastic) sedimentary slope systems in the rock record have been identified mostly as linearly-consistent carbonate apron deposits, even though most ancient clastic carbonate slope deposits fit the submarine fan systems better. Calciclastic submarine fans are consequently rarely described and are poorly understood. Subsequently, very little is known especially in mud-dominated calciclastic submarine fan systems. Presented in this study are a sedimentological core and petrographic characterisation of samples from eleven boreholes from the Lower Carboniferous of Bowland Basin (Northwest England) that reveals a >250 m thick calciturbidite complex deposited in a calciclastic submarine fan setting. Seven facies are recognised from core and thin section characterisation and are grouped into three carbonate turbidite sequences. They include: 1) Calciturbidites, comprising mostly of highto low-density, wavy-laminated bioclast-rich facies; 2) low-density densite mudstones which are characterised by planar laminated and unlaminated muddominated facies; and 3) Calcidebrites which are muddy or hyper-concentrated debrisflow deposits occurring as poorly-sorted, chaotic, mud-supported floatstones. These

9,929 citations

01 Jan 2000
TL;DR: This annex is aimed at providing a sound basis for conclusions regarding the number of significant radiation accidents that have occurred, the corresponding levels of radiation exposures and numbers of deaths and injuries, and the general trends for various practices, in the context of the Committee's overall evaluations of the levels and effects of exposure to ionizing radiation.
Abstract: NOTE The report of the Committee without its annexes appears as Official Records of the General Assembly, Sixty-third Session, Supplement No. 46. The designations employed and the presentation of material in this publication do not imply the expression of any opinion whatsoever on the part of the Secretariat of the United Nations concerning the legal status of any country, territory, city or area, or of its authorities, or concerning the delimitation of its frontiers or boundaries. The country names used in this document are, in most cases, those that were in use at the time the data were collected or the text prepared. In other cases, however, the names have been updated, where this was possible and appropriate, to reflect political changes. Scientific Annexes Annex A. Medical radiation exposures Annex B. Exposures of the public and workers from various sources of radiation INTROdUCTION 1. In the course of the research and development for and the application of atomic energy and nuclear technologies, a number of radiation accidents have occurred. Some of these accidents have resulted in significant health effects and occasionally in fatal outcomes. The application of technologies that make use of radiation is increasingly widespread around the world. Millions of people have occupations related to the use of radiation, and hundreds of millions of individuals benefit from these uses. Facilities using intense radiation sources for energy production and for purposes such as radiotherapy, sterilization of products, preservation of foodstuffs and gamma radiography require special care in the design and operation of equipment to avoid radiation injury to workers or to the public. Experience has shown that such technology is generally used safely, but on occasion controls have been circumvented and serious radiation accidents have ensued. 2. Reviews of radiation exposures from accidents have been presented in previous UNSCEAR reports. The last report containing an exclusive chapter on exposures from accidents was the UNSCEAR 1993 Report [U6]. 3. This annex is aimed at providing a sound basis for conclusions regarding the number of significant radiation accidents that have occurred, the corresponding levels of radiation exposures and numbers of deaths and injuries, and the general trends for various practices. Its conclusions are to be seen in the context of the Committee's overall evaluations of the levels and effects of exposure to ionizing radiation. 4. The Committee's evaluations of public, occupational and medical diagnostic exposures are mostly concerned with chronic exposures of …

3,924 citations