Author
Hisashi Tamura
Bio: Hisashi Tamura is an academic researcher from Westerly Hospital. The author has contributed to research in topics: Immunohistochemistry & Estrogen receptor. The author has an hindex of 1, co-authored 1 publications receiving 10 citations.
Papers
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TL;DR: Data is presented on the immunohistochemical classification of 153 cases of human breast carcinomas as ER-positive (IF+) or ER-negative (IF-), and the relationship between quantity of ER and the proportion of IF+/IF- tumor cells are presented.
10 citations
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TL;DR: Semiquantitative oestrogen receptor assay in formalin-fixed paraffin sections of human breast cancer tissue using monoclonal antibodies as mentioned in this paper was performed in the field of cancer immunology.
Abstract: Semiquantitative oestrogen receptor assay in formalin-fixed paraffin sections of human breast cancer tissue using monoclonal antibodies
62 citations
TL;DR: An immunohistochemical method using a monoclonal antibody to localize estrogen receptors (ER) in formalin-fixed, paraffin-embedded tissue and the effect of enzyme preincubation showed this to increase antigenic expression of ER in formaldehyde-fixed breast tumors and in formsaldehyde-, glutaraldehyde-, and Zamboni-fixed rabbit uteri.
Abstract: We describe an immunohistochemical method using a monoclonal antibody to localize estrogen receptors (ER) in formalin-fixed, paraffin-embedded tissue. The avidin-biotin-peroxidase complex method was used, preceded by trypsin treatment to expose antigenic sites. In 111 breast cancer specimens studied simultaneously by a dextran-coated charcoal (DCC) assay and the paraffin section method, agreement on receptor status was found in 101 (91%) specimens. Quantitative staining features showed a high degree of correlation with the results of the steroid binding assay (r = 0.81). Studies on the influence of fixation on ER localization done in rabbit uteri showed that fixatives mainly composed of coagulating reagents (Carnoy's, Zenker's, Bouin's, Lilly's AAF, Helly's, ethanol) precluded ER staining, whereas cross-linking fixatives (formaldehyde, glutaraldehyde) preserved antigenic sites, although the immunoreactivity of the receptor was somewhat decreased. Studies on the effect of enzyme preincubation showed this to increase antigenic expression of ER in formaldehyde-fixed breast tumors and in formaldehyde-, glutaraldehyde-, and Zamboni-fixed rabbit uteri.
58 citations
TL;DR: The heterogenous nature of breast tumours complicates the application of oestrogen receptor assay results to the management of breast cancer and much effort has been expended on the development of histochemical assays to detect receptors in tissue sections.
Abstract: The heterogenous nature of breast tumours (Hawkins et al., 1977; Silfversward et al., 1980) complicates the application of oestrogen receptor assay results to the management of breast cancer. Much effort has therefore been expended on the development of histochemical assays to detect receptors in tissue sections, initially with oestrogenprotein conjugates (e.g. Pertschuk et al., 1979; Lee, 1978 and Walker et al., 1980) and more recently with antibodies against the receptor protein, both polyclonal (e.g. Lope-Pihie et al., 1985; Tamura et al., 1984) and monoclonal (Greene et al., 1980; Coffer & King, 1981). Few of these methods have been universally accepted or validated in other centres (e.g. McCarthy et al., 1980; Chamness, et al., 1980). In
38 citations
TL;DR: There is as yet no substantial evidence that histochemical methods visualize steroid receptors or detect steroid hormone‐responsiveness of tumour cells, but recent developments suggest that immunocytochemical of receptors and specific hormone‐induced proteins, flow cytometric analysis and probably hybridocytochemistry in the future will help to improve management of steroids hormone‐dependent tumours.
Abstract: The growth of neoplasms originating from steroid hormone target tissues may be hormone-dependent. It has been clearly demonstrated that the oestrogen and/or progestagen receptor status predicts the response to endocrine treatment and the prognosis of breast cancer. However steroid receptor determination in tumour cytosols has several limitations, which can partly be resolved by (immuno) histochemical detection of steroid hormone-responsive tumour cells. A variety of histochemical techniques including autoradiography of tritiated steroids, immunohistochemistry with antibodies to steroid hormones, histochemistry with fluorescent ligands of low molecular weight and fluorochrome- or enzyme-labelled macromolecular probes are discussed. It is concluded that there is as yet no substantial evidence that these methods visualize steroid receptors or detect steroid hormone-responsiveness of tumour cells. On the other hand, immunohistochemical demonstration of oestrogen receptors with monospecific antibodies seems validated and indications have been obtained that this information is clinically relevant. Recent developments suggest that immunocytochemistry of receptors and specific hormone-induced proteins, flow cytometric analysis and probably hybridocytochemistry in the future will help to improve management of steroid hormone-dependent tumours.
13 citations
TL;DR: It is demonstrated that a change in the concentration of formaldehyde alters the staining pattern yielding cytoplasmic instead of nuclear staining in calf uterus, MCF-7 cells, and ER(+) human breast cancer.
8 citations