Holden T. Maecker

TL;DR: It is proposed here that the name tetraspanins be used for the purpose of standardization, and it is proposed that these functions may all relate to their ability to act as “molecular facilitators,” grouping specific cell‐surface proteins and thus increasing the formation and stability of functional signaling complexes.

TL;DR: In the peripheral blood mononuclear cells of COVID-19 patients, the authors observed reduced expression of human leukocyte antigen class DR (HLA-DR) and proinflammatory cytokines by myeloid cells as well as impaired mammalian target of rapamycin (mTOR) signaling and interferon-α (IFN-α) production by plasmacytoid dendritic cells.

TL;DR: The current state of standardization of flow cytometry assays is outlined, which remains highly variable with regard to sample handling, reagents, instrument setup and data analysis, and the steps required to enable the Human Immunology Project are summarized.

TL;DR: A systems-level analysis of 210 healthy twins between 8 and 82 years of age found that 77% of parameters, including cell population frequencies, cytokine responses, and serum proteins, are dominated by non-heritable influences, and in MZ twins discordant for cytomegalovirus infection, more than half of all parameters are affected.

TL;DR: These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community providing the theory and key practical aspects offlow cytometry enabling immunologists to avoid the common errors that often undermine immunological data.