Hongbin Liu

TL;DR: A linear dynamic range over 2 orders of magnitude is demonstrated by using the number of spectra (spectral sampling) acquired for each protein by the data-dependent acquisition of peptides eluting into the mass spectrometer.

TL;DR: Functional dissection of the midbody demonstrated the importance of lipid rafts and vesicle trafficking pathways in cytokinesis, and the utilization of common membrane cytoskeletal components in diverse morphogenetic events in the cleavage furrow, the germline, and neurons.

TL;DR: A computer program called RelEx is described, which uses a least-squares regression for the calculation of the peptide ion current ratios from the mass spectrometry-derived ion chromatograms, and which improves the accuracy of the quantitative measurement by 32 +/- 4%.

TL;DR: Comparison of data sets revealed that while the genes responsible for assembly and maturation of the spore are tightly regulated in discrete stages, many of the components ultimately found in theSpore are expressed throughout and even before sporulation, suggesting that gene expression during sporulation may be mainly related to the physical construction of the Spore, rather than synthesis of eventual spore content.

TL;DR: The strategy to find fertility factors conserved from C. elegans to mammals achieved its goal: of mouse gene knockouts corresponding to nematode proteins, 37% (7/19) cause male sterility and provides significant opportunity to identify causes of male infertility and targets for male contraceptives.