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Hongyuan Jiang

Bio: Hongyuan Jiang is an academic researcher from Harbin Institute of Technology. The author has contributed to research in topics: Dielectrophoresis & Medicine. The author has an hindex of 22, co-authored 92 publications receiving 1350 citations.


Papers
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Journal ArticleDOI
TL;DR: A spraying-plunging method for preparing cryoelectron microscopy grids with vitreous ice of controllable, highly consistent thickness using a microfluidic device is described and it is demonstrated that the structure can be solved to high resolution with this method of sample preparation.

99 citations

Journal ArticleDOI
TL;DR: The capacitive sensing method was shown to work with bovine sera to differentiate disease-positive samples from negative samples within 2 min, while conventional immunoassay would require multiple processing steps and take hours to complete.

91 citations

Journal ArticleDOI
TL;DR: It is revealed that fixed-potential ICEO exceeding RC charging frequency can adjust the particle trapping position flexibly by generating controllable symmetry breaking in a vortex flow pattern.
Abstract: Position-controllable trapping of particles on the surface of a bipolar metal strip by induced-charge electroosmotic (ICEO) flow is presented herein. We demonstrate a nonlinear ICEO slip profile on the electrode surface accounting for stable particle trapping behaviors above the double-layer relaxation frequency, while no trapping occurs in the DC limit as a result of a strong upward fluidic drag induced by a linear ICEO slip profile. By extending an AC-flow field effect transistor from the DC limit to the AC field, we reveal that fixed-potential ICEO exceeding RC charging frequency can adjust the particle trapping position flexibly by generating controllable symmetry breaking in a vortex flow pattern. Our results open up new opportunities to manipulate microscopic objects in modern microfluidic systems by using ICEO.

76 citations

Journal ArticleDOI
TL;DR: A hybrid method for microparticle separation based on a delicate combination of ICEO focusing and dielectrophoretic deflection is proposed and designed and fabricated a microfluidic chip and separated a mixture consisting of yeast cells and silica particles with an efficiency exceeding 96%.
Abstract: Continuous dielectrophoretic separation is recognized as a powerful technique for a large number of applications including early stage cancer diagnosis, water quality analysis, and stem-cell-based therapy. Generally, the prefocusing of a particle mixture into a stream is an essential process to ensure all particles are subjected to the same electric field geometry in the separation region. However, accomplishing this focusing process either requires hydrodynamic squeezing, which requires an encumbering peripheral system and a complicated operation to drive and control the fluid motion, or depends on dielectrophoretic forces, which are highly sensitive to the dielectric characterization of particles. An alternative focusing technique, induced charge electro-osmosis (ICEO), has been demonstrated to be effective in focusing an incoming mixture into a particle stream as well as nonselective regarding the particles of interest. Encouraged by these aspects, we propose a hybrid method for microparticle separation based on a delicate combination of ICEO focusing and dielectrophoretic deflection. This method involves two steps: focusing the mixture into a thin particle stream via ICEO vortex flow and separating the particles of differing dielectic properties through dielectrophoresis. To demonstrate the feasibility of the method proposed, we designed and fabricated a microfluidic chip and separated a mixture consisting of yeast cells and silica particles with an efficiency exceeding 96%. This method has good potential for flexible integration into other microfluidic chips in the future.

67 citations

Journal ArticleDOI
TL;DR: A microfluidic separation device combining 3D electrodes and vaulted obstacles to continuously separate particles experiencing strong positive dielectrophoresis (DEP) from particles experience weak positive DEP, or from particles experiencing negative DEP.
Abstract: This paper reports a microfluidic separation device combining 3D electrodes and vaulted obstacles to continuously separate particles experiencing strong positive dielectrophoresis (DEP) from particles experiencing weak positive DEP, or from particles experiencing negative DEP. The separation is achieved by first focusing the particle mixture into a narrow stream by a hydrodynamic focusing flow, and then deviating them into different outlets by AC DEP. The vaulted obstacles facilitate the separation by both increasing the non-uniformity of the electric field, and influencing the particles to move in regions strongly affected by DEP. The 3D electrodes give rise to a spatially non-uniform electric field and extend DEP effect to the channel height. Numerical simulations are performed to investigate the effects of the obstacles on electric field distribution and particle trajectories so as to optimize the obstacle height and compare with the experimental results. The performance of the device is assessed by separating 25 μm gold-coated particles from 10 μm particles in different flow rates by positive DEP and negative DEP, and also separating 25 μm gold-coated particles from yeast cells using only positive DEP. The experimental observation shows a reasonable agreement with numerical simulation results.

64 citations


Cited by
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[...]

08 Dec 2001-BMJ
TL;DR: There is, I think, something ethereal about i —the square root of minus one, which seems an odd beast at that time—an intruder hovering on the edge of reality.
Abstract: There is, I think, something ethereal about i —the square root of minus one. I remember first hearing about it at school. It seemed an odd beast at that time—an intruder hovering on the edge of reality. Usually familiarity dulls this sense of the bizarre, but in the case of i it was the reverse: over the years the sense of its surreal nature intensified. It seemed that it was impossible to write mathematics that described the real world in …

33,785 citations

01 May 2005

2,648 citations

15 Feb 2016
TL;DR: Standard methods for the examination of water and wastewater: including bottom sediments and sludges, standard methods for examining water, including bottom sediment and sludge, are described in this paper.
Abstract: Standard methods for the examination of water and wastewater: including bottom sediments and sludges , Standard methods for the examination of water and wastewater: including bottom sediments and sludges , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی

613 citations

01 Jan 2016
TL;DR: The hydraulic control systems is universally compatible with any devices to read and is available in the book collection an online access to it is set as public so you can get it instantly.
Abstract: Thank you for downloading hydraulic control systems. As you may know, people have look numerous times for their chosen readings like this hydraulic control systems, but end up in infectious downloads. Rather than reading a good book with a cup of tea in the afternoon, instead they are facing with some infectious bugs inside their laptop. hydraulic control systems is available in our book collection an online access to it is set as public so you can get it instantly. Our book servers saves in multiple countries, allowing you to get the most less latency time to download any of our books like this one. Merely said, the hydraulic control systems is universally compatible with any devices to read.

425 citations

Journal ArticleDOI
TL;DR: Many passive mixers have been proposed to facilitate sample mixing in microfluidic devices over the past five years as mentioned in this paper, and the details of these mixers are briefly described in the following review.

359 citations