Howard Ochman

TL;DR: Unlike eukaryotes, which evolve principally through the modification of existing genetic information, bacteria have obtained a significant proportion of their genetic diversity through the acquisition of sequences from distantly related organisms.

TL;DR: The feasibility of IPCR is shown by amplifying the sequences that flank an IS1 element in the genome of a natural isolate of Escherichia coli.

TL;DR: The evolution of virulence is linked to bacterial sex because rates of evolution have accelerated in pathogenic lineages, culminating in highly virulent organisms whose genomic contents are altered frequently by increased rates of homologous recombination.

TL;DR: Methodes d'extraction d'enzymes, d'electrophorese en gel and de coloration specifique des enzymes utilisees pour etudier la variation genetique chez Escherichia coli et d'autres bacteries.

TL;DR: In this paper, the authors found that 16S rDNA diversity is grossly overestimated unless relatively stringent read quality filtering and low clustering thresholds are applied, and suggested that stringent quality-based trimming of 16S pyrotags and clustering threshold no greater than 97% identity should be used to avoid overestimates of the rare biosphere.