Hsu-Yang Lee

TL;DR: In this article, the authors presented the results of characterization and application of single fluorescent nanodiamonds as cellular biomarkers, and showed that under the same excitation conditions, the fluorescence of a single 35-nm diamond is significantly brighter than that of a simple dye molecule such as Alexa Fluor 546, which was photobleached in the range of 10 s at a laser power density of 104 W/cm2.

TL;DR: The feasibility of using surface-functionalized fluorescent nanodiamonds as single-particle biomarkers is demonstrated with both fixed and live HeLa cells, and the fluorescence of a single 35-nm diamond is significantly brighter than that of asingle dye molecule such as Alexa Fluor 546.

TL;DR: In this article, it was shown that fluorescent nanodiamonds can be produced in large quantities by irradiating synthetic diamond nanocrystallites with helium ions, and the fluorescence is sufficiently bright and stable to allow three-dimensional tracking of a single particle within the cell by means of either one- or two-photon-excited fluorescence microscopy.

TL;DR: It is shown that bright fluorescent nanodiamonds can be produced in large quantities by irradiating synthetic diamond nanocrystallites with helium ions, and the fluorescence is sufficiently bright and stable to allow three-dimensional tracking of a single particle within the cell by means of either one- or two-photon-excited fluorescence microscopy.

TL;DR: Fluorescent nanodiamond contains nitrogen-vacancy defect centers as fluorophores and the intensity of its fluorescence can be significantly enhanced after deposition of the particle on a nanocrystalline Ag film without a buffer layer.