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Hua Zhang

Bio: Hua Zhang is an academic researcher from Chinese Academy of Sciences. The author has contributed to research in topics: Medicine & Graphene. The author has an hindex of 163, co-authored 1503 publications receiving 116769 citations. Previous affiliations of Hua Zhang include Shenzhen University & Zhengzhou University.


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Journal ArticleDOI
TL;DR: A sensitive electrochemical DNA biosensor based on the amplification of Au nanoparticles (AuNPs) has been developed and the presence of MB-T10-SH reduces the cross-reaction between target DNA and MB-probe 2-SH on the AuNPs, resulting in increased sensitivity of the biosensor.
Abstract: A sensitive electrochemical DNA biosensor based on the amplification of Au nanoparticles (AuNPs) has been developed. The AuNPs were modified with two types of signaling reporter DNAs, i.e. a methylene blue probe (MB-probe 2-SH) and T10 with a methylene blue signaling molecule (MB-T10-SH), forming DNA–AuNP conjugates. The MB-probe 2-SH is complementary to the target DNA, while MB-T10-SH is not. The presence of MB-T10-SH reduces the cross-reaction between target DNA and MB-probe 2-SH on the AuNPs, resulting in increased sensitivity of the biosensor. In our assay, the DNA sensor is fabricated by immobilizing a capture probe on the surface of the Au electrode, which then hybridizes with the corresponding target DNA, and further hybridizes with a DNA–Au conjugate. The signal of MB is measured by differential pulse voltammetry, while the DNA–Au conjugate enables the detection of target DNA in the linear range of 10−13 to 10−8 M with the detection limit as low as 50 fM.

23 citations

Journal ArticleDOI
Hua Zhang1, Yang Jj, Zhang Sa, Zhang Sm, Wang Jx, Xu Zy, Lin Ry 
TL;DR: HIF-1α was overexpressed in COPD, which upregulated expressions of inflammatory factors via activating the EGFR/PI3K/AKT pathway, thus aggravating COPD pathological changes.
Abstract: Objective Chronic obstructive pulmonary disease (COPD) is an incomplete, reversible disease with progressive inflammation obstruction in airways. This study aims to explore the regulatory mechanism of hypoxia-inducible factor-1α (HIF-1α) in inflammatory response and progression of COPD. Patients and methods 71 bronchoalveolar lavage fluid (BALF) samples were collected, including 59 samples from COPD patients (COPD group) and 12 from patients with normal pulmonary function (control group). The mRNA and protein levels of HIF-1α and epidermal growth factor receptor (EGFR) in BALF were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) and Western blot, respectively. Serum levels of interleukin-13 (IL-13), IL-9, IL-1, and tumor necrosis factor-α (TNF-α) were detected by enzyme-linked immunosorbent assay (ELISA). Hypoxia cell model was constructed by COCl2 induction in human embryonic lung cells. Expression levels of HIF-1α, EGFR and p-AKT in NCI-H1563 cells treated with 740Y-P, the phosphoinositide 3-kinase (PI3K) agonist were detected. Finally, we detected proliferation and apoptosis in NCI-H1563 cells with HIF-1α overexpression by cell counting kit-8 (CCK-8) assay and flow cytometry, respectively. Results The mRNA and protein levels of HIF-1α and EGFR were higher in COPD groups compared with those of control group. Serum levels of IL-13, IL-9, IL-1, and TNF-α in COPD patients were elevated. CoCl2 induction in NCI-H1563 cells led to upregulated levels of IL-13, IL-9, IL-1, and TNF-α. 740Y-P treatment remarkably activated EGFR/PI3K/AKT pathway. Overexpressed HIF-1α inhibited proliferation but induced apoptosis of NCI-H1563 cells. Conclusions HIF-1α was overexpressed in COPD, which upregulated expressions of inflammatory factors via activating the EGFR/PI3K/AKT pathway. The activated EGFR/PI3K/AKT pathway induced by pulmonary inflammation further upregulated HIF-1α expression in a feedback loop, thus aggravating COPD pathological changes.

23 citations

Journal ArticleDOI
08 Jul 2013-Small
TL;DR: A gold nanotip array platform with a combination of ultrasensitive electrochemical sensing and spectroscopic monitoring capability is reported, which will provide the capability for a better understanding of complex biological processes.
Abstract: A gold nanotip array platform with a combination of ultrasensitive electrochemical sensing and spectroscopic monitoring capability is reported. Adenosine triphosphate is detected down to 1 pM according to the impedance changes in response to aptamer-specific binding. Furthermore, the local molecular information can be monitored at the individual plasmonic nanotips, and hence provide the capability for a better understanding of complex biological processes.

23 citations

Journal ArticleDOI
18 Sep 2009-Small
TL;DR: Benefiting frommultiple transport pathways and without tube– tube overlapping junction problems, densely aligned singlewalled carbon-nanotube (SWCNT)-based nanoelectronic devices show a superior performance to that of their random-network counterparts.
Abstract: Benefiting frommultiple transport pathways andwithout tube– tube overlapping junction problems, densely aligned singlewalled carbon-nanotube (SWCNT)-based nanoelectronic devices show a superior performance to that of their random-network counterparts. The construction of such thin-film transistors (TFTs) has received increasing attention due to their promisingpotential applications ine-paper, flexible displays, and security devices. Controlled growth of highdensity and well-aligned CNTs is an important step for such applications. It has been proven that the atomic step-induced and surface lattice-guided growth approach is one of the most efficient ways for producing aligned SWCNTs. For example, by using single-crystal quartz as substrate, Rogers et al. were able to fabricate large-scalewell-alignedCNTarrays by chemical vapor deposition (CVD). In a recent report, they suggested that the unreacted catalyst particles were likely to cause an adverse effect that would disturb the alignment of CNTs grownon quartz. They demonstrated that in the catalystpatterned region, SWCNTs presented random-network geometrywith a lowdegree of alignment; out of the catalyst region, the SWCNTs adopted a nearly perfect aligned geometry with high density ( 1 tube mm ). Therefore, predefining catalyst into an ordered structure is very important for the growth of dense and well-aligned SWCNT arrays. Several groups have demonstrated that the traditional patterning methods, including photolithography and microcontact printing (mCP), can be used to successfully generate catalyst patterns for the growth of SWCNT arrays. However, all these techniques exhibit various limitations. For

23 citations

Journal ArticleDOI
TL;DR: An interesting, facile and versatile strategy to encapsulate various nanoscale metal oxides into an ultrathin Ni matrix (metal oxide@Ni) for superior LIBs and can be further extended to make other advanced oxide@metal hybrids, not only for LIBs but also for other potential fields.
Abstract: Li-ion batteries’ (LIBs) performance proves to be highly correlated with ionic and electrical transport kinetics in electrodes. Although continual progress has been achieved in rational design of ideal electrode systems, their energy density, cyclic endurance and productivity are still far from perfect for practical use. Herein we propose an interesting, facile and versatile strategy to encapsulate various nanoscale metal oxides (covering both nanopowders and nanostructured arrays) into an ultrathin Ni matrix (metal oxide@Ni) for superior LIBs. Evolutions of such metal oxide@Ni hybrids (taking MnO@Ni and CoO@Ni as models) are thoroughly studied by monitoring their whole fabrication process. Putting “armors” on nanoscale metal oxides is thought helpful for the promotion of the LIB performance since the outer Ni matrix provides both mechanical protection against huge volume changes and effective routes for electron transfer. As a proof-of-concept demonstration, all metal oxide@Ni hybrid electrodes exhibit drastic improvements in the capacity retention (e.g. ∼452% capacity rise for the MnO@Ni case while ∼551% for CoO@Ni NWs), long-term cyclic stability and rate capabilities. This designed strategy can be further extended to make other advanced oxide@metal hybrids, not only for LIBs but also for other potential fields.

23 citations


Cited by
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08 Dec 2001-BMJ
TL;DR: There is, I think, something ethereal about i —the square root of minus one, which seems an odd beast at that time—an intruder hovering on the edge of reality.
Abstract: There is, I think, something ethereal about i —the square root of minus one. I remember first hearing about it at school. It seemed an odd beast at that time—an intruder hovering on the edge of reality. Usually familiarity dulls this sense of the bizarre, but in the case of i it was the reverse: over the years the sense of its surreal nature intensified. It seemed that it was impossible to write mathematics that described the real world in …

33,785 citations

01 May 1993
TL;DR: Comparing the results to the fastest reported vectorized Cray Y-MP and C90 algorithm shows that the current generation of parallel machines is competitive with conventional vector supercomputers even for small problems.
Abstract: Three parallel algorithms for classical molecular dynamics are presented. The first assigns each processor a fixed subset of atoms; the second assigns each a fixed subset of inter-atomic forces to compute; the third assigns each a fixed spatial region. The algorithms are suitable for molecular dynamics models which can be difficult to parallelize efficiently—those with short-range forces where the neighbors of each atom change rapidly. They can be implemented on any distributed-memory parallel machine which allows for message-passing of data between independently executing processors. The algorithms are tested on a standard Lennard-Jones benchmark problem for system sizes ranging from 500 to 100,000,000 atoms on several parallel supercomputers--the nCUBE 2, Intel iPSC/860 and Paragon, and Cray T3D. Comparing the results to the fastest reported vectorized Cray Y-MP and C90 algorithm shows that the current generation of parallel machines is competitive with conventional vector supercomputers even for small problems. For large problems, the spatial algorithm achieves parallel efficiencies of 90% and a 1840-node Intel Paragon performs up to 165 faster than a single Cray C9O processor. Trade-offs between the three algorithms and guidelines for adapting them to more complex molecular dynamics simulations are also discussed.

29,323 citations

28 Jul 2005
TL;DR: PfPMP1)与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用,在黏附及免疫逃避中起关键的作�ly.
Abstract: 抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1(PfPMP1)与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用,在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员,通过启动转录不同的var基因变异体为抗原变异提供了分子基础。

18,940 citations

Journal ArticleDOI
TL;DR: This work reviews the historical development of Transition metal dichalcogenides, methods for preparing atomically thin layers, their electronic and optical properties, and prospects for future advances in electronics and optoelectronics.
Abstract: Single-layer metal dichalcogenides are two-dimensional semiconductors that present strong potential for electronic and sensing applications complementary to that of graphene.

13,348 citations