Author
Huarong Zhou
Bio: Huarong Zhou is an academic researcher from Nanjing University of Science and Technology. The author has contributed to research in topics: Vanillic acid & Yarrowia. The author has an hindex of 2, co-authored 4 publications receiving 12 citations.
Papers
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TL;DR: Yarrowia lipolytica strain is a promising cell factory for the conversion of lignocellulose to bio fuels and bioproducts Despite the inherent robustness of this strain, further improvements to lignoenculose-derived inhibitors toxicity tolerance of Y lipolyteica are also required to achieve industrial application Here, adaptive laboratory evolution was employed with increasing concentrations of ferulic acid as mentioned in this paper.
Abstract: Yarrowia lipolytica strain is a promising cell factory for the conversion of lignocellulose to biofuels and bioproducts Despite the inherent robustness of this strain, further improvements to lignocellulose-derived inhibitors toxicity tolerance of Y lipolytica are also required to achieve industrial application Here, adaptive laboratory evolution was employed with increasing concentrations of ferulic acid The adaptive laboratory evolution experiments led to evolve Y lipolytica strain yl-XYL + *FA*4 with increased tolerance to ferulic acid as compared to the parental strain Specifically, the evolved strain could tolerate 15 g/L ferulic acid, whereas 05 g/L ferulic acid could cause about 90% lethality of the parental strain Transcriptome analysis of the evolved strain revealed several targets underlying toxicity tolerance enhancements YALI0_E25201g, YALI0_F05984g, YALI0_B18854g, and YALI0_F16731g were among the highest upregulated genes, and the beneficial contributions of these genes were verified via reverse engineering Recombinant strains with overexpressing each of these four genes obtained enhanced tolerance to ferulic acid as compared to the control strain Fortunately, recombinant strains with overexpression of YALI0_E25201g, YALI0_B18854g, and YALI0_F16731g individually also obtained enhanced tolerance to vanillic acid Overall, this work demonstrated a whole strain improvement cycle by "non-rational" metabolic engineering and presented new targets to modify Y lipolytica for microbial lignocellulose valorization KEY POINTS: • Adaptive evolution improved the ferulic acid tolerance of Yarrowia lipolytica • Transcriptome sequence was applied to analyze the ferulic acid tolerate strain • Three genes were demonstrated for both ferulic acid and vanillic acid tolerance
28 citations
TL;DR: In this article, an efficient biocatalyst was developed to selectively produce gallate from lignin components by integrating them into a single product, which is a promising way to upgrade Lignin.
Abstract: Converting lignin components into a single product is a promising way to upgrade lignin. Here, an efficient biocatalyst was developed to selectively produce gallate from lignin components by integr...
27 citations
TL;DR: In this paper, through adaptive laboratory evolution on three representative aromatic aldehyde inhibitors, evolved strains of Y. lipolytica XYL+ were obtained, which provided insights to the aromatic acid degradation in Y.lipolyticas and a reliable basis for the development of inhibitor tolerant strains.
22 citations
TL;DR: In this paper, the obscure vanillin metabolic distribution in Rhodoccous opacus PD630 was deciphered by combining the strategies of intermediate detection, putative gene prediction, and target gene verification.
6 citations
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TL;DR: This work intends to fill the gap by retracing the genealogy of the main Yarrowia lipolytica strains of industrial interest, by illustrating the search for new genetic backgrounds and by providing data about the main publicly available strains in yeast collections worldwide.
Abstract: Among non-conventional yeasts of industrial interest, the dimorphic oleaginous yeast Yarrowia lipolytica appears as one of the most attractive for a large range of white biotechnology applications, from heterologous proteins secretion to cell factories process development. The past, present and potential applications of wild-type, traditionally improved or genetically modified Yarrowia lipolytica strains will be resumed, together with the wide array of molecular tools now available to genetically engineer and metabolically remodel this yeast. The present review will also provide a detailed description of Yarrowia lipolytica strains and highlight the natural biodiversity of this yeast, a subject little touched upon in most previous reviews. This work intends to fill this gap by retracing the genealogy of the main Yarrowia lipolytica strains of industrial interest, by illustrating the search for new genetic backgrounds and by providing data about the main publicly available strains in yeast collections worldwide. At last, it will focus on exemplifying how advances in engineering tools can leverage a better biotechnological exploitation of the natural biodiversity of Yarrowia lipolytica and of other yeasts from the Yarrowia clade.
49 citations
TL;DR: In this paper, the authors reviewed the research progress on natural xylose-utilization pathways and their reconstruction in Yarrowia lipolytica for producing chemicals and fuels and discussed challenges and future perspectives of using lignocellulosic hydrolysate as substrate for Y.
25 citations
TL;DR: In this paper, through adaptive laboratory evolution on three representative aromatic aldehyde inhibitors, evolved strains of Y. lipolytica XYL+ were obtained, which provided insights to the aromatic acid degradation in Y.lipolyticas and a reliable basis for the development of inhibitor tolerant strains.
22 citations
TL;DR: Yarrowia lipolytica possesses natural and engineered traits that make it a good host for the industrial bioproduction of chemicals, fuels, foods, and pharmaceuticals as discussed by the authors .
20 citations
TL;DR: A review of recent developments in microbial communities for lignocellulose bioconversion can be found in this article , where the authors discuss different modes of microbial interaction and their molecular mechanisms, and discuss considerations of how to employ these interactions to construct synthetic consortia via synthetic ecology.
Abstract: Lignocellulose is the most abundant organic carbon polymer on the earth. Its decomposition and conversion greatly impact the global carbon cycle. Furthermore, it provides feedstock for sustainable fuel and other value-added products. However, it continues to be underutilized, due to its highly recalcitrant and heterogeneric structure. Microorganisms, which have evolved versatile pathways to convert lignocellulose, undoubtedly are at the heart of lignocellulose conversion. Numerous studies that have reported successful metabolic engineering of individual strains to improve biological lignin valorization. Meanwhile, the bottleneck of single strain modification is becoming increasingly urgent in the conversion of complex substrates. Alternatively, increased attention has been paid to microbial consortia, as they show advantages over pure cultures, e.g., high efficiency and robustness. Here, we first review recent developments in microbial communities for lignocellulose bioconversion. Furthermore, the emerging area of synthetic ecology, which is an integration of synthetic biology, ecology, and computational biology, provides an opportunity for the bottom-up construction of microbial consortia. Then, we review different modes of microbial interaction and their molecular mechanisms, and discuss considerations of how to employ these interactions to construct synthetic consortia via synthetic ecology, as well as highlight emerging trends in engineering microbial communities for lignocellulose bioconversion.
17 citations