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Huizhen Zhi

Bio: Huizhen Zhi is an academic researcher from Nanjing Normal University. The author has contributed to research in topics: AP site & Ionic liquid. The author has an hindex of 2, co-authored 5 publications receiving 21 citations.

Papers
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Patent
21 Nov 2012
TL;DR: In this paper, a method for preparing aluminum hypophosphite was proposed, which includes the following steps of firstly, preparing sodium hypoph phosphite solution with concentration of 2-6mol/L, adjusting pH (potential of hydrogen) to be 3-5 preferentially within 3-6.5.
Abstract: The invention relates to a method for preparing aluminum hypophosphite. The method includes the following steps of firstly, preparing sodium hypophosphite solution with concentration of 2-6mol/L, adjusting pH (potential of hydrogen) to be 3-5 preferentially within 3-6.5; secondly, preparing aluminum sulfate solution; and thirdly, subjecting the sodium hypophosphite solution and the aluminum sulfate solution to reaction by heating on the acid condition to generate aluminum hypophosphite. The aluminum sulfate is added into the sodium hypophosphite solution for reaction within a certain temperature range, so that harms caused by volatilization of hypophosphorous acid during preparing of the aluminum hypophosphite are avoided, operation is simplified, yield of products is high, cost is low and industrialization is easy to realize.

12 citations

Patent
05 Dec 2012
TL;DR: In this article, a preparation method for synthesizing phosphotriester under the catalysis of Lewis acid ionic liquid is presented, where the synthesis of the phosphotryter is generated by performing ring-opening reaction of phosphorus oxychloride and an epoxide under the catalytic activation of a catalyst.
Abstract: The invention discloses a preparation method for synthesizing phosphotriester under the catalysis of Lewis acid ionic liquid. According to the method, the phosphotriester is generated by performing ring-opening reaction of phosphorus oxychloride and an epoxide under the catalysis of a catalyst, wherein ionic liquid [A]-x[Lewis acid] formed by the catalyst is Lewis acid and A; and A is amine and a derivative thereof, imidazole or a derivative thereof or pyridine and a derivative thereof. According to the preparation method, the catalyst is low in cost of raw materials, high in catalytic efficiency, little in using amount and environment-friendly, and a process is simple; and the prepared phosphate flame retardant product is high in quality, small in acid value, good in color and high in purity.

7 citations

Journal ArticleDOI
Huang Xiaodong1, He Zhenni1, Kejie Zhou1, Huizhen Zhi1, Jinfei Yang1 
28 Oct 2021-Analyst
TL;DR: In this paper, the authors extend the loop sequences of G-quadruplex structures and find that G-DNAzymes with long loops (even 30 nucleotides) maintain high peroxidase activity.
Abstract: G-quadruplex-based complexes have been widely used in various analytical methods due to their outstanding capabilities of generating colorimetric, fluorescent or electrochemical signals. However, since loop sequences in traditional G-quadruplex structures are quite short, it is difficult to establish biosensors solely using G-quadruplex-based complexes. Herein, we attempted to lengthen the loop sequences of G-quadruplex structures and found that G-quadruplex-hemin DNAzymes (G-DNAzymes) with long loops (even 30 nucleotides) maintain high peroxidase activity. In addition, the peroxidase activity is not affected by the hybridization of the long loop with its complementary counterpart. Consequently, G-DNAzyme can be endowed with an additional function by taking the long loop as a recognition element, which may facilitate the construction of diverse colorimetric biosensors. Furthermore, by designing an apurinic/apyrimidinic site or a complementary sequence of microRNA-21 (miRNA-21) in long loops, bifunctional G-DNAzymes can be split in the presence of apurinic/apyrimidinic endonuclease 1 (APE1) or miRNA-21, decreasing their peroxidase activities. Accordingly, APE1 and miRNA-21 are quantified using 3,3',5,5'-tetramethylbenzidine as a chromophore. Using the G-DNAzyme, APE1 can be detected in a linear range from 2.5 to 22.5 U mL-1 with a LOD of 1.8 U mL-1. It is to be noted that benefitting from duplex-specific nuclease-induced signal amplification, the linear range of the miRNA-21 biosensor is broadened to 5 orders of magnitude, while the limit of detection is as low as 73 fM. This work demonstrates that G-DNAzymes with long loops can both generate signals and recognize targets, providing an alternative strategy to design G-quadruplex-based analytical methods.

5 citations

Journal ArticleDOI
01 Dec 2017

5 citations

Journal ArticleDOI
TL;DR: In this paper, a solvothermal synthesis strategy was proposed to prepare Rh nanocubes (NCs) with specific exposed Rh (1/0/0) planes, which exhibited higher catalytic performance than commercial Rh black toward hydrazine hydrate oxidation reaction (HHOR) and hydrogen evolution reaction (HER).

1 citations


Cited by
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Journal ArticleDOI
TL;DR: Wang et al. as mentioned in this paper detected miRNA-21 and APE1 in two modes, AND and OR, respectively, based on gold nanoflares and simple logic components.
Abstract: Compared with the single-marker detection scheme, the detection of multiple targets in the complex cell and biological environment can obtain more reliable detection results. Herein, we detected miRNA-21 and APE1 in two modes, AND and OR, respectively, based on gold nanoflares and simple logic components. In both modes, DNAzyme and APE1 can get rich fluorescence recovery results by breaking the DNA strands from the gold nanorods (AuNRs) and unquenching under different conditions. In vivo and in vitro experiments suggest that both nanoflares exhibit excellent biocompatibility and make efficient and sensitive judgments on the two targets. This strategy emphasizes the reuse nature of enzymes, and a small amount of target can generate a large amount of fluorescent signal in the logic device, which greatly reduces the detection limit when monitoring low-abundance targets. Since the short-stranded DNA component of the detection device is simple in composition and easy to program its probe sequence, it can be expanded into a detection system for the detection of other sets of related markers, which increases its potential for clinical application.

13 citations

Journal ArticleDOI
TL;DR: The use of organophosphorus compounds plays an important role in the modern chemical industry and have a broad range of applications as flame retardants, agrochemicals, and chemical warfare agents as mentioned in this paper .

12 citations

Patent
12 Jun 2013
TL;DR: In this paper, a preparation method of aluminium hypophosphite is presented, which consists of: a, preparing a sodium hypoph phosphite solution; b, preparing an aluminium nitrate solution; and c, uniformly mixing the sodium hypphosphite solution with the aluminium nitric solution, regulating the pH (potential of Hydrogen) to 2-6, heating and reacting to form the target product aluminium pyrophosphite.
Abstract: The invention discloses a preparation method of aluminium hypophosphite. The preparation method comprises the following steps of: a, preparing a sodium hypophosphite solution; b, preparing an aluminium nitrate solution; and c, uniformly mixing the sodium hypophosphite solution with the aluminium nitrate solution, regulating the pH (potential of Hydrogen) to 2-6, heating and reacting to form the target product aluminium hypophosphite. The method disclosed by the invention has the advantages that the reaction is carried out through directly taking water as solvent and the temperature is lower, so that the reaction is easy to control; the particle size of the obtained aluminium hypophosphite is less than 50 micrometers, the aluminium hypophosphite is white powder and no extra color processing is needed, so that the purity is higher and the yield of the obtained target product is 92-99%; the conventional synthetic process route is simplified and no toxic substance is produced, so that the preparation method is friendly to environment and low in cost; and high-efficiency fire retardant and combustion improver are obtained in the same reaction, so that the resource utilization rate is high and better industrial prospect is provided.

7 citations

Patent
01 Feb 2017
TL;DR: In this paper, a continuous preparation method of tri(2,3-dichloropropyl)phosphate was proposed, which includes the steps of: according to ratio, continuously adding phosphorus oxychloride, epoxy chloropropane and a catalyst into esterification reaction kettles to perform a reaction.
Abstract: The invention relates to a continuous preparation method of tri(2,3-dichloropropyl)phosphate, which includes the steps of: according to ratio, continuously adding phosphorus oxychloride, epoxy chloropropane and a catalyst into esterification reaction kettles to perform a reaction, wherein the esterification reaction is carried out at different temperatures in different reaction kettles, thereby obtaining an esterification reaction crude product in an auto-overflow manner between the reaction kettles; and water-washing and neutralizing-washing the crude product and performing pressure reduced dehydration to obtain a tri(2,3-dichloropropyl)phosphate-TDCP finish product. The method allows continuous production of the tri(2,3-dichloropropyl)phosphate-TDCP, is low in labor intensity, has simple operations and short production period, is economical and environment-friendly, is high in production efficiency, can satisfy demands in industrial-scale production, and is suitable for being industrially promoted.

6 citations