Showing papers by "I. S. Bright Singh published in 2001"
TL;DR: This is the first report of a cell culture system from the ovarian tissues of African catfish, and the cell cultures can be maintained for 12–15 days without fluid change between the passages.
33 citations
01 Jan 2001
TL;DR: For the first time in India a cell culture system from the eye stalk of Penaeus indicus has been developed by a simple technique and on repeated trials 100% reproducibility could be obtained and the cultures developed could attain 100% confluency in plasdc bottles.
Abstract: For the first time in India a cell culture system from the eye stalk of Penaeus indicus has been developed by a simple technique. On repeated trials 100% reproducibility could be obtained and the cell cultures developed could attain 100% confluency in plasdc bottles. The medium used for the development consisted of Leibovitz (L-15) 45%, (2X) (HIMEDIA, Bombay), supplemented with 18% foetal bovine serum (FBS), 27% prawn muscle extract (PME), 10% prawn haemolymph (PHL) and antibiodc mixture consisdng of penicillin, streptomycin sulphate and chloramphenicol. pH of the medium was adjusted to 6.8 to 7.2 and the osmolarity 720 ± 10 mOsm and the temperature of incubadon was 25°C. The cell culture development could be attained in closed system as well as in open system in a CO2 incubator in an atmosphere of 5% CO2. Interesdngly in all instances cell proliferadon and migration from the expiants and formation of monolayer could commence within 24 hours and the cultures developed could be maintained for more than 60 days without fluid change. The cells developed were epitheloid like with intracellular granules.
7 citations
Journal Article•
TL;DR: The effects of temperature, wet/dry packing and oxygen on the survival of live clams (Meretrix casta) were studied in this paper, where an insulated box was fabricated and clams were chill-stored at varying temperatures from 11 to 22°C.
Abstract: The effects of temperature, wet/dry packing and oxygen on the survival of live clams (Meretrix casta), were studied. The survival period was longest in the temperature range of 10-20°C. Packing clams in polyethylene bags with water and oxygen had little effect on the extension of the survival period. An insulated box was fabricated and clams were chill-stored at varying temperatures from 11 to 22°C. Clams survived for a period of 7-8 days when chill-stored, whereas these survived only for a period of 3-4 days at room temperature. Les effets de la temperature, de l'humidite de l'oxygene sur la survie de la palourde sont mesures. La temperature optimale est de 10-20°. Des emballages polyethylene avec de l'eau et de l'oxygene ne permettent pas d'ameliorer la survie. Une survie des palourdes de 7 a 8 jours dans une enceinte isolee de 11 a 22° peut etre obtenue, alors qu'a temperature ambiante elles ne survivront que 3 a 4 jours.
2 citations