Showing papers by "I. S. Bright Singh published in 2021"
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TL;DR: In this article, the surface morphology, roughness, thermal and mechanical properties of 3-hydroxybutyrate-co-3-hydroxymethylpolyvalerate (PHBV) films were characterized.
8 citations
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TL;DR: The results showed that the chitosan-induced flocculation offers an efficient, cost-effective, rapid, and sustainable harvesting method for C. muelleri biomass for food and feed applications in aquaculture.
Abstract: Microalgae are a promising new source of biomass; however, large-scale economical harvesting of microalgal biomass is a major technological and economic challenge, limiting the commercial production of microalgal biomass for high-value compounds. In this study, the cationic polymer chitosan was used for the harvesting of the marine diatom Chaetoceros muelleri. Natural flocculation, and pH and chitosan-induced flocculation were studied in detail. The effects of flocculant dosage, culture pH, initial biomass concentration, and sedimentation time were investigated on biomass recovery. The results showed that flocculation efficiency can reach > 99% with an optimum dosage of chitosan (80 mg L−1) at pH 9.6 and settling time of 40 minutes for biomass concentration from 0.2 to 1.2 g L−1. The reusability of the recycled water, preservation of biomass after harvesting, and cost of the harvesting process were evaluated. The results showed that the chitosan-induced flocculation offers an efficient, cost-effective, rapid, and sustainable harvesting method for C. muelleri biomass for food and feed applications in aquaculture.
5 citations
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TL;DR: In this article, an isolate of actinomycete MCCB 110 was segregated based on its comparatively higher inhibitory property on Vibrio harveyi (MCCB 111) and profound luminescent inhibition.
5 citations
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TL;DR: In this paper, the authors reported an isoform of the anti-lipopolysaccharide factor (Mm-ALF) from the speckled shrimp, Metapenaeus monoceros, and the sequence showed 9916% similarity to ALF from the shrimp Fenneropenaeus indicus.
Abstract: Anti-lipopolysaccharide factors (ALFs) are antimicrobial peptides of approximately 100 amino acid residues with a broad spectrum of antimicrobial activity It is an amphipathic peptide with an N-terminal hydrophobic region and a lipopolysaccharide binding domain (LBD) In the present study, we report an isoform of the anti-lipopolysaccharide factor (Mm-ALF) from the speckled shrimp, Metapenaeus monoceros A 359 bp cDNA encoded 119 amino acids, and the sequence showed 9916% similarity to ALF from the shrimp Fenneropenaeus indicus The mature peptide of 94 amino acids has a net charge of +8, molecular weight 1062 kDa, and pI 1011 The mature peptide Mm-ALF was recombinantly expressed in E coli Rosetta-gami cells, and the peptide was isolated and purified The rMm-ALF exhibited notable antibacterial activity against Gram-positive (Staphylococcus aureus and Bacillus cereus) and Gram-negative (Escherichia coli, Edwardsiella tarda, Aeromonas hydrophila, Pseudomonas aeruginosa, Vibrio parahaemolyticus, Vibrio harveyi, Vibrio alginolyticus, Vibrio proteolyticus, Vibrio cholerae and Vibrio fluvialis) bacteria
3 citations
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TL;DR: In this article, a β-defensin was detected from the gill mRNA of a red-toothed trigger fish, Odonus niger, and a cDNA was synthesized, and the β-Defensin isoform was cloned and subjected to structural and functional characterization in silico.
3 citations
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01 Apr 2021
TL;DR: This study paves the way for understanding the microbial communities, specifically the ammonia oxidizers responsible for maintaining healthy and optimal environmental conditions in the bioaugmented zero-water exchange shrimp culture pond.
Abstract: In the zero-water exchange shrimp culture pond maintained with the application of indigenous bioaugmentor, low levels of total ammonia–nitrogen were reported, indicating the relevance of indigenous microbial communities. Sediments (0–5 cm layer) were sampled from the pond (85th day) and the bacterial and archaeal communities; specifically, the ammonia oxidizers (ammonia-oxidizing bacteria, ammonia-oxidizing archaea, and anaerobic ammonia-oxidizing bacteria) in the sediment metagenome of the pond were analysed using the 16S rRNA and functional genes. Bacterial and archaeal 16S rRNA genes showed the relative abundance of Delta-Proteobacteria and Bacteroidetes groups performing sulphur respiration and organic matter degradation, archaeal groups of anaerobic sulphur respiring Crenarchaeotae, and chemolithoautotrophic ammonia oxidizers belonging to Thaumarchaeota. The presence of these diverse bacterial and archaeal communities denotes their significant roles in the cycling the carbon, nitrogen, and sulphur thereby bringing out efficient bioremediation in the bioaugmented zero-water exchange shrimp culture pond. Similarly, the functional gene-specific study showed the predominance of Nitrosomonas sp. (ammonia-oxidizing bacteria), Nitrosopumilus maritimus (ammonia-oxidizing archaea), and Candidatus Kuenenia (anaerobic ammonia-oxidizing bacteria) in the system, which points to their importance in the removal of accumulated ammonia. Thus, this study paves the way for understanding the microbial communities, specifically the ammonia oxidizers responsible for maintaining healthy and optimal environmental conditions in the bioaugmented zero-water exchange shrimp culture pond.
1 citations