I. S. Bright Singh

Bio: I. S. Bright Singh is an academic researcher from Cochin University of Science and Technology. The author has contributed to research in topics: Penaeus monodon & Shrimp. The author has an hindex of 24, co-authored 120 publications receiving 1876 citations.

Optimum growth requirements of nitrifying consortia developed from treated sewage.

Abstract: The optimum growth requirements of two nitrifying consortia developed from treated sewage by enrichment technique were determined by a series of experiments. There was total inhibition of nitrification at above 2.75 g r' NH/- Nand 2.5g r' N02' - N and the ammonia oxidizing consortium preferred a pH at 8.5 and the nitrite oxidizing consortium a pH of 7.5 as the optima for nitrification. Optimum temperatures were between 20° and 30°C for both the groups. As the rate of airnow was increased from I to 7 Ilmin, the build-up of N02- -N increased 10-fold and the consumption of N02- -N increased by a factor o f 28.8 implying that the ammonia oxidizing consortium in a bioreactor required three times more aeration than th at for nitrite oxidizers for ex press ing their full nitrifying potential. These data directly contribute for developing a fermentati o n process for the mass production of nitrifiers as well as for designing bi oreactors for nitrifying sewage.

Bioprocess technology for LasB protease (elastase) production from Pseudomonas aeruginosa MCCB 123

Abstract: LasB protease, also known as the elastase enzyme was purified from an environmental isolate of Pseudomonas aeruginosa MCCB 123. The enzyme production was optimized by response surface methodology (RSM) which yielded 24754.17 U/mL protease activity and 654.14 U/ml elastase activity. The yields were 1.83 fold and 2.39 fold higher for protease and elastase activities, respectively. Scale up study of enzyme production was carried in a 5 L bioreactor. Bioprocess technology for enzyme production was successfully developed.

Design, Fabrication and Standardisation of a Depuration System for Bivalves

Abstract: A depuration system was designed and fabricated for cleaning of bivalves. The unit consisted of a depuration chamber, biological filter and UV sterilizer. The system was evaluated based on trial studies conducted using Villorita cyprinoides . Escherichia coli was used as the indicator bacteria. Seeding of the animals by bacteria was carried out using sea water of 10%o salinity and a bacterial concentration of 1x10' cfu ml'1. On 6 h exposure of clams to the E. coli cell suspension, seeding was achieved to the desired level (6.69 x 10" cfu g"1)- On depuration of seeded V. cyprinoides for a period of 15 h using the depuration system complete elimination of E. coli could be achieved.

The Design and Analysis of Experiments

Abstract: THE DESIGN AND ANALYSIS OF EXPERIMENTS. By Oscar Kempthorne. New York, John Wiley and Sons, Inc., 1952. 631 pp. $8.50. This book by a teacher of statistics (as well as a consultant for \"experimenters\") is a comprehensive study of the philosophical background for the statistical design of experiment. It is necessary to have some facility with algebraic notation and manipulation to be able to use the volume intelligently. The problems are presented from the theoretical point of view, without such practical examples as would be helpful for those not acquainted with mathematics. The mathematical justification for the techniques is given. As a somewhat advanced treatment of the design and analysis of experiments, this volume will be interesting and helpful for many who approach statistics theoretically as well as practically. With emphasis on the \"why,\" and with description given broadly, the author relates the subject matter to the general theory of statistics and to the general problem of experimental inference. MARGARET J. ROBERTSON