Igawa Yoji

Bio: Igawa Yoji is an academic researcher. The author has contributed to research in topics: Gene & Genome. The author has an hindex of 1, co-authored 2 publications receiving 3 citations.

Method for sorting out homologous recombinant cell

Abstract: PURPOSE:To obtain a method for sorting out a homologous recombinant cell, applicable even to a gene unexpressed with an embryonic undifferentiated (ES) cell and having a high sorting out efficiency. CONSTITUTION:The objective method for sorting out a homologous recombinat cell is characterized by using a genome DNA comprising a neo gene having a PGK promoter on the upstream side thereof and an MVM pause signal and a blue script DNA inserted into a region between the neo gene and an MC1 promoter of a diphtherial toxin A fragment gene in a method for homologously recombining the genome DNA in which the neo gene and the diphtherial toxin A fragment gene having the MC1 promoter integrated therein in an embryonic undifferentiated (ES) cell and sorting out a cell containing a genome DNA having a deficient or modified gene therein with G418 and diphtherial toxin A.

Embryo-undifferentiated cell

Abstract: PURPOSE:To provide an embryo-undifferentiated (ES) cell originated from a mouse of a strain other than 129 mouse and capable of efficiently producing descendant mice. CONSTITUTION:The objective embryo-undifferentiated (ES) cell originated from a 4-days embryo obtained by crossing a female of C57BL/6 mouse with a male of CBA mouse and characterized by the generation of chimera mouse from a 3-days embryo (8-cell embryo) and free from generation of chimera mouse from a 4-days embryo. The ES cell is e.g. TT2 embryo undifferentiated (ES) cell of FERM P-12971.

Methods of increasing rates of infection by directing motion of vectors

Abstract: This invention provides methods of increasing the frequency of contact between vectors and stationary target cells in an apparatus containing them which involves causing the vectors to move towards the target cells with motion above and beyond random Brownian motion. The methods of this invention include causing the vectors to move in the direction of the cells by (1) causing flow-through of a liquid containing the vectors through or past a cell bed, (2) moving charged vectors towards the target cells by electrodiffusion and (3) centrifuging vectors and cells to cause settling of vectors onto the cells.

Methods, compositions and apparatus for cell transfection

Abstract: This invention provides methods, compositions and apparatus for increasing the transfection efficiency of target cells by particles, especially retroviral particles, compared with that achieved by current methods The transfection method comprises depositing the particles on a cell growth support and contacting target cells with the particle-loaded cell growth support

Pluripotent cells having disrupted intrinsic genes

Abstract: A process for constructing a chimeric non-human animal characterized by preparing microcells containing one or more foreign chromosomes or fragments thereof, and transferring the one or more foreign chromosomes or fragments thereof into pluripotent cells through fusion with the microcells; chimeric non-human animals which can be constructed by the above process and offspring thereof; tissues and cells originating in the above animals; methods for using the individuals, tissues and cells of the animals; pluripotent cells containing one or more foreign chromosomes or fragments thereof, a process for constructing these cells, and the use of these cells; pluripotent cells in which at least two intrinsic genes have been disrupted; a process for constructing the above cells by homologous recombination; etc.