Inge S. Zuhorn

TL;DR: The data indicate that the size itself of (ligand-devoid) particles can determine the pathway of entry in non-phagocytic B16 cells, and kinetic parameters may determine the almost exclusive internalization of such particles along this pathway rather than via caveolae.

TL;DR: In addition to triggering endocytosis, UMTD also evoked transient pore formation, as demonstrated by the influx of calcium ions and cellular release of preloaded dextrans after US and microbubble exposure.

TL;DR: It is concluded that efficient lipoplex-mediated transfection requires complex internalization via the cholesterol-dependent clathrin-mediated pathway of endocytosis, and that caveolae-mediated internalization can be excluded.

TL;DR: Investigation of the interaction of lipo- and polyplexes with HeLa cells by live cell imaging reveals that in contrast to the involvement of numerous nanocarriers in case of lipoplex-mediated delivery, only a very limited number ofpolyplexes contribute to the release of nucleic acids from endosomes and their subsequent accumulation into the nucleus.

TL;DR: It is demonstrated that although hexagonal phase formation in lipoplexes is a prerequisite for nucleic acid release from the complex, it appears highly critical for accomplishing efficient translocation of nucleic acids across the endosomal membrane into the cytosol for transport to the nucleus.