J. A. Jongejan

Bio: J. A. Jongejan is an academic researcher. The author has contributed to research in topics: Butyrate & Transesterification. The author has an hindex of 1, co-authored 1 publications receiving 2 citations.

Strategies in the Preparation of Homochiral Compounds Using Combined Enantioselective Enzymes

Abstract: In order to obtain a homochiral product from a racemic substrate, different strategies can be followed using a moderately enantioselective enzymatic catalyst. Two new strategies are presented, involving the simultaneous use of two enzymes, parallel or consecutive. In the parallel system, the substrate enantiomer yielding the unwanted product enantiomer is enantioselectively converted by the second enzyme. In the consecutive system, the substrate enantiomer yielding the desired product enantiomer is itself the preferred product of another enantioselective enzymatic reaction.For irreversible pseudo-first order enzyme kinetics, a relationship was found which describes the dependency of the yield and enantiomeric excess for these systems on the E-values of the separate enzymes and on the ratio of their concentrations. For Michaelis-Menten kinetics, these relationships usually give good approximations.According to these calculations, the yield and enantiomeric excess obtainable with the concepts of combined en...

Process for preparing optically active 3-phenylglycidic acid esters

Abstract: There is disclosed a process for preparing optically active 3-phenylglycidic acid ester compound, which comprises permitting a culture broth, cells or treated cells of a microorganism having an ability of stereoselectively hydrolyzing a (2R, 3S)-3-phenylglycidic acid ester compound to act on a racemic 3-phenylglycidic acid ester compound which may also have a substituent on the phenyl group, thereby hydrolyzing the (2R, 3S) optically active isomer and separating and collecting the (2S, 3R) antipode from the reaction mixture.