Author
J.A. Meath
Bio: J.A. Meath is an academic researcher from Harvard University. The author has contributed to research in topics: Myelin. The author has an hindex of 3, co-authored 3 publications receiving 1447 citations.
Topics: Myelin
Papers
More filters
TL;DR: A simple method is described for the preparation of extracts of total pure lipides from brain tissue by homogenizing the tissue with a chloroform-methanol mixture and washing free of non-lipide contaminants.
1,203 citations
TL;DR: The isolation from brain tissue of a substance to which the name of strandin has been given for descriptive purposes is reported, which has the property of forming long strands that show perfect orientation under polarized light.
253 citations
Journal Article•
21 citations
Cited by
More filters
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
59,550 citations
TL;DR: The lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials that has been applied to fish muscle and may easily be adapted to use with other tissues.
Abstract: Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can...
46,099 citations
TL;DR: This chapter discusses the different aspects of thiobarbituric acid assay of sialic acid, which is suitable for measuring the release of bound sialoic acid by sialidase and hydrolysis of sIALic acid-containing material must be carried out for the measurement of total sialsic acids.
6,264 citations
TL;DR: Study of the cell surface binding, internalization, and metabolism of low-density lipoprotein (LDL) in cultured cells have provided useful information regarding the general aspects of receptor-mediated endocytosis and three classes of mutant alleles at the LDL receptor locus have been deduced.
Abstract: Publisher Summary Studies of the cell surface binding, internalization, and metabolism of low-density lipoprotein (LDL) in cultured cells have provided useful information regarding the general aspects of receptor-mediated endocytosis. The study of the LDL receptor has been facilitated by analysis of mutant fibroblasts obtained from human subjects with disorders of cholesterol metabolism. The most informative cells, obtained from patients with familial hypercholesterolemia (FH), have defects in the gene encoding the LDL receptor. The existence of three classes of mutant alleles at the LDL receptor locus has been deduced on the basis of genetic and kinetic data. One of these alleles specifies a receptor that is unable to bind LDL. The second type of allele specifies a receptor that can bind small amounts of LDL; and the third type of allele specifies a receptor that can bind LDL but cannot be incorporated into coated pits and hence cannot carry the LDL into the cell. The first two alleles are common among FH patients, whereas the third allele is extremely rare.
1,589 citations
TL;DR: A simple method is described for the preparation of extracts of total pure lipides from brain tissue by homogenizing the tissue with a chloroform-methanol mixture and washing free of non-lipide contaminants.
1,203 citations