The vitamin content in four Australian microalgae, a Nannochloropsis-like sp., Pavlova pinguis, Stichococcus sp. and Tetraselmis sp., were examined. These were grown under a 12:12 h light:dark regimen (100 μmol photon m−2s−1) and harvested during late-logarithmic phase. Typically, the content showed a two- to three fold range between the species. When expressed on a dry weight basis, the content of ascorbate ranged from 1.3 to 3.0 mg g−1, β-carotene from 0.37 to 1.05 mg g−1, α-tocopherol from 0.07 to 0.29 mg g−1, thiamine from 29 to 109 μg g−1, riboflavin from 25 to 50 μg g−1, total folates from 17 to 24 μg g−1, pyridoxine from 3.6 to 17 μg g−1, cobalamin from 1.70 to 1.95 μg g−1 and biotin from 1.1 to 1.9 μg g−1. Retinol was detected only in Tetraselmis sp. (2.2 μg g−1); any vitamins D2 or D3 were below the detection limit (≤0.45 μg g−1). Nannochloropsis sp. was also grown under a 24:0 h light:dark light cycle and harvested at stationary phase. The content of most vitamins in Nannochloropsis sp. cultures differed significantly, and the degree of variation was similar to that observed between the four species grown under 12:12 h light:dark regimen (100 μmol photon m−2s−1) and harvested during late-logarithmic phase. Thiamine content was also examined in six non-Australian strains commonly used in aquaculture, Chaetoceros muelleri, Thalassiosira pseudonana, Nannochloris atomus, Nannochloropsis oculata, Isochrysis sp. (T.ISO) and Pavlova lutheri. Values (average 61 μg g−1; range 40 to 82) were similar to those in the Australian strains (average 61 μg g−1; range 29 to 109) and increased during stationary phase (average 94 μg g−1; 38 to 131). Comparison of the data with the known nutritional requirements for marine fish species and prawns suggests that the microalgae should provide excess or adequate levels of the vitamins for aquaculture food chains. The data may be used to guide the content of vitamins included in micro-diets developed as replacements for live diets.

The vitamin content of microalgae used in aquaculture

Determination of water-soluble vitamins in infant milk by high-performance liquid chromatography

http://www.treehealthcare.co.uk/wp-content/uploads/2013/10/13-RuizRodriguezMadronoFRI2011.pdf

Valorization of wild strawberry-tree fruits (Arbutus unedo L.) through nutritional assessment and natural production data

Extraction procedures for the liquid chromatographic determination of thiamin, riboflavin and vitamin B6 in foodstuffs.

Water-soluble vitamins include the B-group vitamins and vitamin C In order to correctly monitor water-soluble vitamin content in fortified foods for compliance monitoring as well as to establish accurate data banks, an accurate and precise analytical method is a prerequisite For many years microbiological assays have been used for analysis of B vitamins However they are no longer considered to be the gold standard in vitamins analysis as many studies have shown up their deficiencies This review describes the current status of analytical methods, including microbiological assays and spectrophotometric, biosensor and chromatographic techniques In particular it describes the current status of the official methods and highlights some new developments in chromatographic procedures and detection methods An overview is made of multivitamin extractions and analyses for foods and supplements

Analytical procedures for water-soluble vitamins in foods and dietary supplements: a review

Liquid chromatographic determination of vitamins B1 and B2 in foods. A collaborative study

Determination of vitamin B6 in foods by HPLC : a collaborative study

Une analyse interlaboratoire a ete realisee afin d'evaluer une methode chromatographique de dosage de la vitamine C totale dans les aliments, comportant une reduction precolonne de l'acide dehydro L-ascorbique en acide L-ascorbique par la L-cysteine. Quinze participants ont analyse six aliments contenant des teneurs variees en vitamine C (de 35,6 a 169,3 mg/100 g). Les teneurs initiales en acide dehydro L-ascorbique, non significatives dans deux aliments (jus d'orange et cereales pour petit dejeuner), etaient modestes, mais non negligeables dans les quatre autres. Les valeurs des deviations standard relatives de reproductibilite (RSD R ), independantes de la concentration en vitamine, ont ete comprises entre 11,4 et 21,6 %. Quel que soit l'aliment etudie, le taux de recouvrement de la methode a toujours ete superieur a 90 %. La methode proposee pourrait etre retenue comme methode officielle de dosage de la vitamine C dans les aliments destines a une alimentation particuliere, en remplacement de la methode fluorimetrique a l'orthophenylenediamine.

Application de la chromatographie en phase liquide haute performance à la détermination de l'acide L-ascorbique et de la vitamine C totale dans les aliments. Etude interlaboratoire

J.B. Bourguignon

Papers

Liquid chromatographic determination of vitamins B1 and B2 in foods. A collaborative study

Determination of vitamin B6 in foods by HPLC : a collaborative study

Application de la chromatographie en phase liquide haute performance à la détermination de l'acide L-ascorbique et de la vitamine C totale dans les aliments. Etude interlaboratoire