J. Keith Wright

TL;DR: Upon induction of pTE18-harbouring strains the Y-gene product is expressed at a nearly constant rate for several generations and accumulates to a level of 12-16% of the total cytoplasmic membrane protein.

TL;DR: The author’s personal experiences, as well as those of a number of other researchers, have led him to believe that the current state of public transport in the United States is in need of improvement.

TL;DR: The orientation of the lactose:H+ carrier of Escherichia coli in various preparations of native and reconstituted vesicles is determined with two impermeant, macromolecular probes: antibodies directed against the C-terminal decapeptide of the carrier and carboxypeptidase A and anti-(C-terminus) antibodies.

TL;DR: Comparison of the steady-state level of galactoside accumulation and the nominal value of the driving gradients yielded cotransport stoichiometries up to 0.7 proton/galactosid, suggesting that the carrier protein is the only component required for active galactOSide transport.

TL;DR: Lactose permease is a highly hydrophobic protein of the cytoplasmic membrane of E. coli which mediates the active transport of galactosides in response to an electrochemical proton gradient, an attractive model for studies on the molecular mechanism of a solute: H + symporter.