J
J. Zhang
Researcher at Merck & Co.
Publications - 9
Citations - 358
J. Zhang is an academic researcher from Merck & Co.. The author has contributed to research in topics: Indene & Enantiomeric excess. The author has an hindex of 6, co-authored 9 publications receiving 344 citations.
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Journal ArticleDOI
Chemically defined media for commercial fermentations
J. Zhang,Randolph Greasham +1 more
TL;DR: In this paper, the authors focus on the application, development, and practical considerations of fermentations in chemically defined media in an industrial environment and present a review of the application and process economics of such media.
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Development of a defined medium fermentation process for physostigmine production by Streptomyces griseofuscus.
J. Zhang,C. Marcin,Shifflet Ma,P. Salmon,T Brix,Randolph Greasham,Barry C. Buckland,Michel Chartrain +7 more
TL;DR: A fermentation process that used chemically defined medium for physostigmine production and a titer of 880 mg/l was attained during scale-up in a 800-l fermentor by employing a nutrient-feeding strategy was very amenable to downstream processing.
Journal ArticleDOI
Chiral Bio-Resolution of Racemic Indene oxide by Fungal Epoxide Hydrolases
J. Zhang,Jay Reddy,Christopher Roberge,Chris H. Senanayake,Randolph Greasham,Michel Chartrain +5 more
TL;DR: In this article, 80 fungal strains were evaluated for their production of epoxide hydrolase capable of catalyzing the kinetic resolution of racemic indene oxide into 1(S),2(R) indeneoxide.
Journal ArticleDOI
Development of a bioconversion process for production of cis-1S,2R-indandiol from indene by recombinant Escherichia coli constructs.
TL;DR: Recombinant Escherichia coli cells expressing the toluene dioxygenase (TDO) genes from Pseudomonas putida convert indene to cis-1S,2R-indandiol, a potentially important intermediate for the chemical synthesis of the HIV-1 protease inhibitor, Crixivan.
Journal ArticleDOI
Bioconversion of indene to trans-2S,1S-bromoindanol and 1S,2R-indene oxide by a bromoperoxidase/dehydrogenase preparation from Curvularia protuberata MF5400
J. Zhang,Christopher Roberge,J. Reddy,N. Connors,Michel Chartrain,Barry C. Buckland,Randolph Greasham +6 more
TL;DR: It was found that a crude enzyme preparation from the fungal culture Curvularia protuberata MF5400 converted indene to the chiral 2S,1S-bromoindanol which can be chemically converted to the desired 1S,2R-epoxide through basification or used directly in the asymmetric synthesis of cis -1S, 2R-aminoindanol.