J
Jacques J. Smith
Researcher at University of the Western Cape
Publications - 6
Citations - 3479
Jacques J. Smith is an academic researcher from University of the Western Cape. The author has contributed to research in topics: Promoter & Gene. The author has an hindex of 4, co-authored 4 publications receiving 3349 citations.
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Review and re-analysis of domain-specific 16S primers
TL;DR: This work re-assess the specificity of commonly used 16S rRNA gene primers and presents these data in tabular form designed as a tool to aid simple analysis, selection and implementation.
Review and re-analysis of domain-specific 16S primers (Review article)
TL;DR: In this article, the specificity of commonly used 16S rRNA gene primers was evaluated and two new primers were designed for effective 'universal' Archaeal16S rDNA sequence amplification.
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Bacterial Diversity in Three Different Antarctic Cold Desert Mineral Soils
TL;DR: The detection of a substantial number of uncultured bacterial phylotypes showing low BLAST identities (<95%) suggests that Antarctic Dry Valley mineral soils harbor a pool of novel psychrotrophic taxa.
Journal ArticleDOI
Metagenomics, gene discovery and the ideal biocatalyst.
Don A. Cowan,A. Arslanoglu,Stephanie G. Burton,Gillian C. Baker,Rory A. Cameron,Jacques J. Smith,Quinton Meyer +6 more
TL;DR: New technologies, which circumvent the limitations of microbial culturability, must be applied to expand the pool genes for in vitro evolution, and provide access to a volume of "sequence space" that is not addressed by traditional screening.
Journal ArticleDOI
Influence of codon optimization, promoter, and strain selection on the heterologous production of a β-fructofuranosidase from Aspergillus fijiensis ATCC 20611 in Pichia pastoris
TL;DR: This study overexpressed two differently codon-optimized variations of the Aspergillus fijiensis β-fructofuranosidase-encoding gene ( fopA) under the transcriptional control of either the alcohol oxidase ( AOX1 ) or glyceraldehyde-3-phosphate dehydrogenase ( GAP ) promoters.