J
Jakob Reiser
Researcher at Center for Biologics Evaluation and Research
Publications - 82
Citations - 8856
Jakob Reiser is an academic researcher from Center for Biologics Evaluation and Research. The author has contributed to research in topics: Transduction (genetics) & Gene. The author has an hindex of 42, co-authored 81 publications receiving 8455 citations. Previous affiliations of Jakob Reiser include National Institutes of Health & University of Zurich.
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Journal ArticleDOI
Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure
TL;DR: A rapid and very sensitive method for detecting proteins as antigens after their separation in polyacrylamide/agarose composite gels, with or without sodium dodecyl sulfate.
Journal ArticleDOI
Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors.
TL;DR: Improved protocols to generate highly concentrated lentiviral vector pseudotypes involving different envelope glycoproteins are described, which result in high-titer vector preparations that show reduced toxicity compared with lentIViral vectors produced using standard protocols involving ultracentrifugation-based methods.
Journal ArticleDOI
Autoinducer-mediated regulation of rhamnolipid biosurfactant synthesis in Pseudomonas aeruginosa
Urs A. Ochsner,Jakob Reiser +1 more
TL;DR: The RhLR-RhlI regulatory system was found to be essential for the production of elastase as well, and cross-communication between the RhlR- RhlI rhamnolipid regulatory system and the LasR-LasIElastase elastases regulatory systemwas demonstrated.
Journal ArticleDOI
Altering the tropism of lentiviral vectors through pseudotyping.
TL;DR: This review samples a few of the more prominent examples from the ever-expanding list of published lentiviral pseudotypes, noting comparisons made with pseudotypes involving VSV-G in terms of titer, viral particle stability, toxicity, and host-cell specificity.
Book ChapterDOI
[15] Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper
James C. Alwine,David J. Kemp,Barbara A. Parker,Jakob Reiser,Jaime Renart,George R. Stark,Geoffrey M. Wahl +6 more
TL;DR: The efficiency of transfer is very high and independent of the size of a fragment as covalent linkage allows convenient multiple reuse of the transfers and also allows washings to be done repeatedly under stringent conditions, thereby helping to achieve very low backgrounds.