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James A. Magnuson

Bio: James A. Magnuson is an academic researcher from Washington State University. The author has contributed to research in topics: Concanavalin A & Membrane. The author has an hindex of 13, co-authored 36 publications receiving 642 citations.
Topics: Concanavalin A, Membrane, Gene, Ion, Peptide sequence

Papers
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Journal ArticleDOI
TL;DR: A cDNA clone of the bovine interleukin 2 (IL-2) gene has been isolated and demonstrated to be functional in the production of secreted bovines IL-2 protein when transfected into monkey cells, suggesting a cell or tissue-specific regulatory function for these evolutionarily conserved sequences.
Abstract: A cDNA clone of the bovine interleukin 2 (IL-2) gene has been isolated and demonstrated to be functional in the production of secreted bovine IL-2 protein when transfected into monkey cells. The bovine IL-2 clone is 791 base pairs in length and contains an open reading frame of 474 base pairs coding for a bovine IL-2 precursor polypeptide of 158 amino acids with an estimated molecular weight of 17,884. The putative hydrophobic leader or signal sequence of the precursor protein is 23 amino acid residues long, suggesting that, after removal by processing, the mature secreted bovine IL-2 protein contains 135 amino acids and has a molecular weight of 15,464. Comparisons of both the nucleotide sequence and the predicted amino acid sequence of bovine IL-2 with those of the human and mouse IL-2 show extensive regions of sequence conservation between the species, interspersed with other regions of less similarity. The 3' untranslated region of the bovine IL-2 gene shares as much, if not greater, sequence homology with the 3' untranslated regions of the human and mouse genes as do the transcribed coding regions of these genes, suggesting an involvement of this region in regulation. In particular, a tandemly repeated sequence, (TATT)n, found in the 3' untranslated tail of the bovine IL-2 clone is also found in the 3' untranslated region of the other known interleukin and interferon genes, as well as in similar regions of many other inducible genes of the lymphoid and immune response systems, suggesting a cell or tissue-specific regulatory function for these evolutionarily conserved sequences.

65 citations

Journal ArticleDOI
TL;DR: Mise en evidence de differences significatives des indices de polarisation de fluorescence du DPH (diphenyl-1,6-hexatriene-2,3,5) des membranes des microsomes de pommes selon qu'elles ont ete entreposees au froid (2 o C) ou a temperature ordinaire et traitees ou non prealablement par le calcium.
Abstract: Mise en evidence de differences significatives des indices de polarisation de fluorescence du DPH (diphenyl-1,6-hexatriene-2,3,5) des membranes des microsomes de pommes selon qu'elles ont ete entreposees au froid (2 o C) ou a temperature ordinaire et traitees ou non prealablement par le calcium

53 citations

Journal ArticleDOI
TL;DR: Using continuous wave NMR spectroscopy, two quadrupolar ions are examined in pea stem cells where about 90% of the ion content is in the largely aqueous vacuoles having a membrane barrier, and the NMR resonances correspond to almost 100% of that expected from independent measurements of total ion content.

47 citations


Cited by
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Book ChapterDOI
TL;DR: This chapter considers only those lectins that have been purified to homogeneity, and studied with regard to their biophysical, biochemical, and carbohydrate-binding specificity.
Abstract: Publisher Summary Lectins play an important role in the development of immunology. Lectins also find application in serological laboratories for typing blood and determining secretor status, separating leucocytes from erythrocytes, and agglutinating cells from blood in the preparation of plasma. They serve as reagents for the detection, isolation, and characterization of carbohydrate-containing macromolecules, including blood-group antigens. In their interaction with saccharides, lectins serve as models for carbohydrate-specific antibodies, with the important advantage to purify lectins in gram quantities. Lectins are classified according to their carbohydrate-binding specificity that includes D-mannose(D-glucose)-binding lectins and 2-acetamido-2-deoxy-D-glucose-binding lectins. The chapter considers only those lectins that have been purified to homogeneity, and studied with regard to their biophysical, biochemical, and carbohydrate-binding specificity. The chapter also describes the cell-binding and biological properties of lectins. The chapter concludes with the description of several glycopeptide structures showing the carbohydrate-binding loci with which various lectins interact.

1,540 citations

Journal ArticleDOI
TL;DR: This review focuses on developments in the last 6-7 years, and cites data resulting from the isolation and characterization of SRC mutants, crystallographic studies of the structures of SH2, SH3 and tyrosine kinase domains, biochemical studies of Src kinase activity and binding properties, and the biology of transgenic and knockout mouse strains.

1,198 citations

Journal ArticleDOI
21 Apr 1989-Science
TL;DR: Data show that stimuli received at the cell surface can alter gene expression by inducing specific changes in messenger RNA degradation, and this affects the steady-state messenger RNA level, transcription, or messenger RNA half-life of other T cell activation genes.
Abstract: Quiescent T cells can be induced to express many genes by mitogen or antigen stimulation. The messenger RNAs of some of these genes undergo relatively rapid degradation compared to messenger RNAs from constitutively expressed genes. A T cell activation pathway that specifically regulates the stability of messenger RNAs for the lymphokines interleukin-2, interferon-gamma, tumor necrosis factor-alpha, and granulocyte-macrophage colony-stimulating factor is induced by stimulation of the CD28 surface molecule. This pathway does not directly affect the steady-state messenger RNA level, transcription, or messenger RNA half-life of other T cell activation genes, including c-myc, c-fos, IL-2 receptor, and the 4F2HC surface antigen. These data show that stimuli received at the cell surface can alter gene expression by inducing specific changes in messenger RNA degradation.

920 citations

Journal ArticleDOI
TL;DR: In this paper, Urea-formaldehyde microcapsules containing dicyclopentadiene were prepared by in situ polymerization in an oil-in-water emulsion that meet these requirements for self-healing epoxy.
Abstract: Microencapsulated healing agents that possess adequate strength, long shelf-life and excellent bonding to the host material are required for self-healing materials. Urea-formaldehyde microcapsules containing dicyclopentadiene were prepared by in situ polymerization in an oil-in-water emulsion that meet these requirements for self-healing epoxy. Microcapsules of 10-1000 microm in diameter were produced by appropriate selection of agitation rate in the range of 200-2000 rpm. A linear relation exists between log(mean diameter) and log(agitation rate). Surface morphology and shell wall thickness were investigated by optical and electron microscopy. Microcapsules are composed of a smooth 160-220 nm inner membrane and a rough, porous outer surface of agglomerated urea-formaldehyde nanoparticles. Surface morphology is influenced by pH of the reacting emulsion and interfacial surface area at the core-water interface. High yields (80-90%) of a free flowing powder of spherical microcapsules were produced with a fill content of 83-92 wt% as determined by CHN analysis.

751 citations

Journal ArticleDOI
TL;DR: The predicted BD peptide structure, which is referred to as the "A.T-hook," represents a previously undescribed DNA-binding motif capable of binding to the minor groove of stretches of A.T base pairs.

669 citations