Jan Ellenberg

TL;DR: Evidence is provided that, while RNF8 is necessary to trigger the DSB-associated ubiquitylation, it is not sufficient to sustain conjugated ubiquitin in this compartment, which defines a new pathway involving sequential ubiquitylations on damaged chromosomes and uncovers a functional cooperation between E3 ligases in genome maintenance.

TL;DR: This study carried out a genome-wide phenotypic profiling of each of the ∼21,000 human protein-coding genes by two-day live imaging of fluorescently labelled chromosomes, which allowed us to identify hundreds of human genes involved in diverse biological functions including cell division, migration and survival.

TL;DR: This work provides a quantitative description of the proteome of a commonly used human cell line in two functional states, interphase and mitosis, and shows that these human cultured cells express at least ∼10 000 proteins and that the quantified proteins span a concentration range of seven orders of magnitude up to 20 000 000 copies per cell.

TL;DR: Selective changes in lateral mobility of LBR–GFP within the ER/NE membrane system form the basis for its localization to the inner nuclear membrane during interphase, and also underlie the redistribution of this molecule during NE disassembly and reformation in mitosis.

TL;DR: It is demonstrated that proteasome-mediated degradation and hER alpha-mediated transactivation are inherently linked and act to continuously turn over hERalpha on responsive promoters.