Je Hyuk Lee

TL;DR: The present review deals with the qualitative, quantitative, microbiological and immunological assays and the comparison of the quantitative methods of vancomycin.

TL;DR: The estA gene encoding the enzyme that catalyzes the production of (R)-beta-acetylmercaptoisobutyric acid from (R,S)-ester from Pseudomonas aeruginosa 1001, was cloned in Escherichia coli and its nucleotide sequence was determined, revealing the presumed open reading frame encoding a polypeptide of 316 amino acid residues.

TL;DR: (R)-β-acetylmercaptoisobutyric acid is an important intermediate for the chemical synthesis of various antihypertensive and congestive heart failure drugs and can be converted asymmetrically from (R,S)-ester.

TL;DR: An expression vector was constructed to overproduce a maltose binding protein (MBP)-esterase fusion protein in Escherichia coli that could not be separated into MBP and esterase by the action of protease 'Factor Xa', and the resulting ester enzyme activity was increased to 34% after cleavage.

TL;DR: The novel esterase and the nucleotide sequence producing optical active RAM, the expression vector overproducing RAM from (R,S)-ester, transformant containing expression vector, and the method of enzyme solution for the production of RAM are disclosed.