Jeff F. Miller

TL;DR: E. coli can be transformed to extremely high efficiencies by subjecting a mixture of cells and DNA to brief but intense electrical fields of exponential decay waveform (electroporation), and most of the surviving cells are competent with up to 80% transformed at high DNA concentration.

TL;DR: This work has shown that proteins that mediate sensory transduction and virulence control are similar to components of other bacterial information processing systems.

TL;DR: It is demonstrated that the application of high-voltage discharges to bacterial cells permits genetic transformation, and evidence is presented that indicates that C. jejuni possesses DNA restriction and modification systems.

TL;DR: The data show that overexpression of the bvgA gene from a strong heterologous promoter results in transcriptional activation of the fhaB::lacZYA fusion even in the absence of thebvgB and bvgC products, which may be important in regulating the activity of BvgA in response to the changing environmental stimuli that B. pertussis encounters during the diseases whooping cough.

TL;DR: A bvg-dependent regulatory system in Escherichia coli is reconstructed by using bacteriophage lambda vectors carrying transcriptional fusions to lacZYA that required an intact bvg locus as determined by 5' and 3' deletions that eliminated both activities.