J
Jenny J. Wei
Researcher at Massachusetts Institute of Technology
Publications - 4
Citations - 7101
Jenny J. Wei is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: Gene & CRISPR. The author has an hindex of 4, co-authored 4 publications receiving 6156 citations.
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Genetic Screens in Human Cells Using the CRISPR-Cas9 System
TL;DR: In this paper, a pooled, loss-of-function genetic screening approach suitable for both positive and negative selection that uses a genome-scale lentiviral single-guide RNA (sgRNA) library was described.
Genetic Screens in Human Cells Using the CRISPR-Cas9 System
TL;DR: A pooled, loss-of-function genetic screening approach suitable for both positive and negative selection that uses a genome-scale lentiviral single-guide RNA (sgRNA) library is described and it is shown that sgRNA efficiency is associated with specific sequence motifs, enabling the prediction of more effective sgRNAs.
Journal ArticleDOI
Identification and characterization of essential genes in the human genome
Timothy C. Wang,Kıvanç Birsoy,Nicholas W. Hughes,Kevin M. Krupczak,Yorick Post,Yorick Post,Jenny J. Wei,Eric S. Lander,Eric S. Lander,Eric S. Lander,David M. Sabatini +10 more
TL;DR: Using the bacterial clustered regularly interspaced short palindromic repeats (CRISPR) system, this article constructed a genome-wide single-guide RNA library to screen for genes required for proliferation and survival in a human cancer cell line.
Identification and characterization of essential genes in the human genome
Timothy C. Wang,Kıvanç Birsoy,Nicholas W. Hughes,Kevin M. Krupczak,Yorick Post,Yorick Post,Jenny J. Wei,Eric S. Lander,Eric S. Lander,Eric S. Lander,David M. Sabatini +10 more
TL;DR: A genome-wide single-guide RNA library is constructed to screen for genes required for proliferation and survival in a human cancer cell line and reveals a set of cell-essential genes, which was validated with an orthogonal gene-trap–based screen and comparison with yeast gene knockouts.