scispace - formally typeset
Search or ask a question
Author

Jere R. Mitchell

Bio: Jere R. Mitchell is an academic researcher. The author has an hindex of 1, co-authored 1 publications receiving 23 citations.

Papers
More filters
Journal ArticleDOI
TL;DR: The information covered in the article includes sample preparation and the use of manual counts, spectrophotometers, computer-assisted semen analysis, NucleoCounter, and flow cytometry.

34 citations


Cited by
More filters
Journal ArticleDOI
TL;DR: This review provides an update on two technologies: flow cytometry and CASA for objective analysis of boar semen quality for high throughput, objective and accurate analysis.

18 citations

01 Jan 2016
TL;DR: The uv visible reflection spectroscopy of liquids is universally compatible with any devices to read and is available in the authors' digital library an online access to it is set as public so you can download it instantly.
Abstract: Thank you very much for downloading uv visible reflection spectroscopy of liquids. As you may know, people have look numerous times for their favorite books like this uv visible reflection spectroscopy of liquids, but end up in harmful downloads. Rather than reading a good book with a cup of tea in the afternoon, instead they juggled with some harmful virus inside their desktop computer. uv visible reflection spectroscopy of liquids is available in our digital library an online access to it is set as public so you can download it instantly. Our book servers saves in multiple countries, allowing you to get the most less latency time to download any of our books like this one. Kindly say, the uv visible reflection spectroscopy of liquids is universally compatible with any devices to read.

13 citations

Journal ArticleDOI
TL;DR: Knowledge on the effect of seminal plasma on sperm function in extended semen during in vitro storage is lacking and the need for further research is needed.
Abstract: BACKGROUND Knowledge on the effect of seminal plasma on sperm function in extended semen during in vitro storage is lacking. OBJECTIVES The aim was to examine the interactive role of sperm concentration and seminal plasma concentration on boar sperm function during in vitro aging. MATERIAL AND METHODS Experiment 1: Twenty-one boar ejaculates were aliquoted with Beltsville Thawing Solution into five semen doses containing between 32.5 and 8.5 × 106 sperm/mL. Experiment 2: Semen samples (n = 8) containing 18 × 106 or 10 × 106 sperm/mL with their natural amount of seminal plasma and 10 × 106 sperm/mL substituted with autologous seminal plasma to the same concentration as in doses with 18 × 106 sperm/mL were prepared. Experiment 3: Four variants of semen doses containing 18 × 106 or 10 × 106 sperm/mL with either 10% or 0.5% (v/v) seminal plasma were used. Lipid peroxidation was assessed using Bodipy 581/591 in samples (n = 8) with two different sperm concentrations. Semen was examined during 144-h storage at 17 °C by computer-assisted semen analysis and flow cytometry. RESULTS Experiment 1: 3D kinematic patterns revealed a concentration- and time-dependent loss of sperm kinematics in samples with 0.05). Experiment 3: At 144 h, motility was lowest in samples containing 10 × 106 sperm/mL and 10% (v/v) seminal plasma (p < 0.05). Sperm lipid peroxidation did not differ between samples with different sperm concentration. CONCLUSION Long-term exposure to seminal plasma has a negative impact on in vitro-aged boar spermatozoa. Reduced sperm-to-sperm proximity but not the reduction of seminal plasma limits sperm function in long-term stored boar semen.

11 citations

Journal ArticleDOI
TL;DR: The effect of human chorionic gonadotropin (hCG) on sperm production, an efficient sperm-to-egg ratio for fertilization, and the use of a densimeter to measure sauger sperm cell concentration for precise sperm addition during fertilization are investigated.

9 citations

Journal ArticleDOI
TL;DR: All parameters were comparable between automated and manual analysis, and repeatability measures confirm the more reliable values of the SCA compared to those of manual analysis.
Abstract: For sperm analysis, important inter-laboratory variations have been observed in manual analyses. In this study, a computer-aided sperm analysis (CASA) system was assessed versus manual technique, and specific software modifications were operated to fit the David's classification already used in the laboratory. Four parameters were studied (concentration, motility, vitality and morphology), and at least 30 semen samples from 30 different patients have been tested. Manual and automated analyses were compared using a least-squares regression line analysis, Student's t test, Bland-Altman plots and Passing-Bablok regressions. Repeatability was also assessed, and coefficients of variation (CV) were calculated. Both manual and automated methods gave similar results for sperm concentration (n = 150), motility (n = 30), vitality (n = 90) and morphology (n = 90). Repeatability always showed a decrease in the CV with automated analysis; for example in normal range of sperm values, CV for manual and CASA analyses were, respectively, 9.0% versus 4.4% for sperm concentration, 5.2% versus 4.1% for motility, 7.3% versus 4.2% for vitality and 11.4% versus 4.1% for morphology. All parameters were comparable between automated and manual analysis, and repeatability measures confirm the more reliable values of the SCA compared to those of manual analysis.

8 citations