Modern digital cameras employ silicon focal plane array (FPA) image sensors featuring millions of pixels. However, it is possible to make a camera that only needs one pixel. In these cameras a spatial light modulator, placed before or after the object to be imaged, applies a time-varying pattern and synchronized intensity measurements are made with a single-pixel detector. The principle of compressed sensing then allows an image to be generated. As the approach suits a wide a variety of detector technologies, images can be collected at wavelengths outside the reach of FPA technology or at high frame rates or in three dimensions. Promising applications include the visualization of hazardous gas leaks and 3D situation awareness for autonomous vehicles. Rather than requiring millions of pixels, it is possible to make a camera that only needs one pixel. This Review details the working principle, advantages, technical considerations and future potential of single-pixel imaging.

Principles and prospects for single-pixel imaging

Review on practical photoacoustic microscopy.

Multiplexing multiple orbital angular momentum (OAM) channels enables high-capacity optical communication. However, optical scattering from ambient microparticles in the atmosphere or mode coupling in optical fibers significantly decreases the orthogonality between OAM channels for demultiplexing and eventually increases crosstalk in communication. Here, we propose a novel scattering-matrix-assisted retrieval technique (SMART) to demultiplex OAM channels from highly scattered optical fields and achieve an experimental crosstalk of –13.8 dB in the parallel sorting of 24 OAM channels after passing through a scattering medium. The SMART is implemented in a self-built data transmission system that employs a digital micromirror device to encode OAM channels and realize reference-free calibration simultaneously, thereby enabling a high tolerance to misalignment. We successfully demonstrate high-fidelity transmission of both gray and color images under scattering conditions at an error rate of <0.08%. This technique might open the door to high-performance optical communication in turbulent environments.

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Optical orbital-angular-momentum-multiplexed data transmission under high scattering

Orbital angular momentum (OAM) has demonstrated great success in the optical communication field, which theoretically allows an infinite increase of the transmitted capacity. The resolution of a receiver to precisely recognize OAM modes is crucial to expand the communication capacity. Here, we propose a deep learning (DL) method to precisely recognize OAM modes with fractional topological charges. The minimum interval recognized between adjacent modes decreases to 0.01, which as far as we know is the first time this superhigh resolution has been realized. To exhibit its efficiency in the optical communication process, we transfer an Einstein portrait by a superhigh-resolution OAM multiplexing system. As the convolutional neuron networks can be trained by data up to an infinitely large volume in theory, this work exhibits a huge potential of generalized suitability for next generation DL based ultrafine OAM optical communication, which might even be applied to microwave, millimeter wave, and terahertz OAM communication systems.

Superhigh-Resolution Recognition of Optical Vortex Modes Assisted by a Deep-Learning Method.

Intratumoral heterogeneity, which is manifested in almost all of the hallmarks of cancer, including the significantly altered metabolic profiles of cancer cells, represents a challenge to effective cancer therapy. High-throughput measurements of the metabolism of individual cancer cells would allow direct visualization and quantification of intratumoral metabolic heterogeneity, yet the throughputs of current measurement techniques are limited to about 120 cells per hour. Here, we show that single-cell photoacoustic microscopy can reach throughputs of approximately 12,000 cells per hour by trapping single cells with blood in an oxygen-diffusion-limited high-density microwell array and by using photoacoustic imaging to measure the haemoglobin oxygen change (that is, the oxygen consumption rate) in the microwells. We demonstrate the capability of this label-free technique by performing high-throughput single-cell oxygen-consumption-rate measurements of cultured cells and by imaging intratumoral metabolic heterogeneity in specimens from patients with breast cancer. High-throughput single-cell photoacoustic microscopy of oxygen consumption rates should enable the faster characterization of intratumoral metabolic heterogeneity.

High-throughput, label-free, single-cell photoacoustic microscopy of intratumoral metabolic heterogeneity

Photoacoustic microscopy (PAM) is uniquely positioned for biomedical applications because of its ability to visualize optical absorption contrast in vivo in three dimensions. Here we propose motionless volumetric spatially invariant resolution photoacoustic microscopy (SIR-PAM). To realize motionless volumetric imaging, SIR-PAM combines two-dimensional Fourier-spectrum optical excitation with single-element depth-resolved photoacoustic detection. To achieve spatially invariant lateral resolution, propagation-invariant sinusoidal fringes are generated by a digital micromirror device. Further, SIR-PAM achieves 1.5 times finer lateral resolution than conventional PAM. The superior performance was demonstrated in imaging both inanimate objects and animals in vivo with a resolution-invariant axial range of 1.8 mm, 33 times the depth of field of the conventional PAM counterpart. Our work opens new perspectives for PAM in biomedical sciences.

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Motionless volumetric photoacoustic microscopy with spatially invariant resolution.

A new laser differential reflection-confocal focal-length measurement (DRCFM) method is proposed for the high-accuracy measurement of the lens focal length. DRCFM uses weak light reflected from the lens last surface to determine the vertex position of this surface. Differential confocal technology is then used to identify precisely the lens focus and vertex of the lens last surface, thereby enabling the precise measurement of the lens focal length. Compared with existing measurement methods, DRCFM has high accuracy and strong anti-interference capability. Theoretical analyses and experimental results indicate that the DRCFM relative measurement error is less than 10 ppm.

Laser differential reflection-confocal focal-length measurement

Optical scattering prevents light from being focused through thick biological tissue at depths greater than ∼1 mm. To break this optical diffusion limit, digital optical phase conjugation (DOPC) based wavefront shaping techniques are being actively developed. Previous DOPC systems employed spatial light modulators that modulated either the phase or the amplitude of the conjugate light field. Here, we achieve optical focusing through scattering media by using polarization modulation based generalized DOPC. First, we describe an algorithm to extract the polarization map from the measured scattered field. Then, we validate the algorithm through numerical simulations and find that the focusing contrast achieved by polarization modulation is similar to that achieved by phase modulation. Finally, we build a system using an inexpensive twisted nematic liquid crystal based spatial light modulator (SLM) and experimentally demonstrate light focusing through 3-mm thick chicken breast tissue. Since the polarization modulation based SLMs are widely used in displays and are having more and more pixel counts with the prevalence of 4 K displays, these SLMs are inexpensive and valuable devices for wavefront shaping.

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Focusing light through scattering media by polarization modulation based generalized digital optical phase conjugation

The invention relates to a method and device for measuring multiple element parameters in differential con-focus (con-focus) interference manner, belonging to the technical field of optical precise measurement. The core thought of the invention is as follows: a differential con-focus (con-focus) measurement system is used for measuring the surface curvature radius of a spherical element, the vertex focal distance of a lens, the refraction index of a lens, the thickness of the lens and the axial clearance of a lens group, and a facial-contour interference measurement system is used for measuring the facial contour of an element surface to realize simultaneous measurement on multiple parameters of an element and high measurement precision. In the invention, the differential con-focus (con-focus) measurement system and the facial-contour interference measurement system are fused for the first time to measure parameters comprehensively; and in the process of measuring the multiple parameters of the element, a light path is unnecessary to regulate and a measured element is unnecessary to detach, thus, the measured element is not damaged and the measurement speed is high.

Jiamiao Yang

Papers

Optical orbital-angular-momentum-multiplexed data transmission under high scattering

Motionless volumetric photoacoustic microscopy with spatially invariant resolution.

Laser differential reflection-confocal focal-length measurement

Focusing light through scattering media by polarization modulation based generalized digital optical phase conjugation

Method and device for measuring multiple element parameters in differential con-focus interference manner