Jiayue Xu

TL;DR: In this article, the effects of GQD and berberine on a diabetic rat model by comprehensive analyses of gut microbiota, short-chain fatty acids, proinflammatory cytokines, and ileum transcriptomics were investigated.

TL;DR: In this article, RNA/cDNA-targeted sequencing (meta-transcriptomics using NGS (mtNGS)) is established to reduce the host nucleotide percentage in clinic samples and by combining with Oxford Nanopore Technology (ONT) platforms, mtTGS is used to improve the sequencing time.

TL;DR: A full working protocol for analyzing human gut virome using physical enrichment, reverse transcription and random amplification, and eventually the state-of-art single-molecule real-time sequencing (SMRT) platform of Oxford Nanopore Technology (ONT).

TL;DR: In this paper, a multi-omics sequencing and analyzing method based on a Nanopore sequencing technology is proposed, which consists of simultaneously performing DNA and RNA extraction on a collected sample, adding a Poly U tail to the 3' end of the extracted RNA, and reverse transcription on the RNA with the PolyU tail to obtain cDNA, and adding an MID barcode sequence to the 5' ends of the cDNA.

TL;DR: A full working protocol for analyzing human gut virome using physical enrichment, reverse transcription and random amplification, and eventually the state-of-art single-molecule real-time sequencing (SMRT) platform of Oxford Nanopore Technology (ONT).